Method for rapidly propagating akebia trifoliata varaustralis seedlings through somatic embryogenesis pathway
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A technology of embryogenesis and somatic embryos, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of high bacterial infection rate and low proliferation efficiency, and achieve low bacterial infection rate and proliferation efficiency of tissue culture. High, low variability effects
Inactive Publication Date: 2021-04-02
江西省·中国科学院庐山植物园
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[0005] The purpose of the present invention is to provide a simple and efficient method for obtaining aseptic seedlings of Akebia in
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Embodiment 1
[0034]A method of rapid breeding of white wood gem seedlings through somatic cell embryo, including the steps, including
[0035]Step (1) Excerpts from 7 to 8 months of unsuccessful white woodfield, which is treated with an unsuccessful seed from the flesh, which includes fruit processing and seed processing;
[0036](1.1) Fruit processing: In January-August, the unreserved spotless white woodworking fruit is collected, and the surface of the fruit surface is brush and put it into the washing solution for 20 min, then rinse with distilled water. 3-5 times, then use filter paper to dry the fruit surface; then put the fruit after cleaning the surface into the ultra-clean workbench, and remove the unripe seeds in the fruit with acknowledgment knife.
[0037](1.2) Seed Treatment: Rinse the extracted seeds for 3-5 times, then use 75% alcohol soaked 30s, then rinse 2-3 times with sterile water, then use 0.1% (w / v) HGCl2The solution was soaked for 7 min, and then rinsed 3 times with sterile water...
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Abstract
The invention discloses a method for rapidly propagating akebia trifoliata varaustralis seedlings through a somatic embryogenesis pathway. According to the method for rapidly propagating the akebia trifoliata varaustralis seedlings through the somatic embryogenesis pathway, immature akebia trifoliata varaustralis seeds are used as explants for inducing somatic embryos. The method mainly has the advantages that 1) zygotic embryos of immature seeds have higher cell totipotency, the somatic embryogenesis process is easily induced, the immature seeds are in a relatively sterile environment in pulp, and the tissue culture contamination rate is low; 2) no exogenous plant hormones need to be added in the process of inducing somatic embryogenesis initiation and subculture multiplication, and initiation induction and subculture multiplication can be carried out in an MS culture medium; and 3) through the somatic embryogenesis pathway, the somatic embryo proliferation efficiency is high, and a large number of akebia trifoliata varaustralis aseptic seedlings can be obtained in a short time; and 4) the induced somatic embryos can be developed into complete plants, are originated from single cells, and have the advantages of low chimerism rate, low mutation rate and the like.
Description
Technical field[0001]The present invention belongs to the field of white wood tissue culture, and in particular, the present invention relates to a method of rapid breeding of white wood gear seedlings by somatic embryos.Background technique[0002]Bai Wai PassAkebia Trifoliata SSP.Australis ) Wooden belonging (LardizaBalaceae)Akebia Multi-years wooden ivil. my country is the distribution center of Bai Wai Tong, its distribution is wide, north to Shaanxi, Shanxi, Henan, south to Yunnan, Guangdong, Guangxi. Its fruit is usually ripe in the lunar calendar. When maturity, the peel is fed along the line. Therefore, it is also become "eight months", "August", but also known as a wild banana, also known as "wild bananas", etc. . Bai Tongfu is not only delicious, sweet and delicious, but also rich in soluble sugar, starch, crude protein, crude fiber, Vc, mineral, and 17 amino acids (Li Li et al, 2010). As the traditional Chinese herbal medicine, Bai Wai Tong has more than 2,000 years of appl...
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