Method for increasing embryogenesis rate of microspores of Wucai

A technology of microspores and occurrence rate, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as hidden dangers, and achieve the effects of safe use, improved embryogenesis rate, and simple operation.

Active Publication Date: 2021-02-23
ANHUI AGRICULTURAL UNIVERSITY +1
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies in the prior art, and provide a method for improving the incidence of microspore embryos in Wucai, so as to solve the technical problem of hidden dangers in the method for increasing the incidence of microspore embryos in Wucai

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  • Method for increasing embryogenesis rate of microspores of Wucai

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Embodiment approach

[0019] 1. Select the full-grained black cabbage variety WS-1 and sow it in the breeding medium. After most of the seeds emerge, perform normal water and fertilizer management. When the seedlings grow to 4-5 true leaves, transplant them into flower pots. The day / night temperature is (25±1)℃ / (18±1)℃, and the photosynthetically active radiation is 300-500μmol·m -2 ·s -1 , The relative humidity is 60-70%, maintain sufficient water and nutrient supply. When the seedlings have 6-7 true leaves, select plants with good growth and no pests and diseases to start spraying with a concentration of 50mg / L BRs. Water was sprayed as a control. The two treatments were sprayed once every 5 days, and the spraying site was the back of the leaves, until the surface dripped, and continued to spray until the early stage of bolting of Chinese cabbage.

[0020] 2. When the plant begins to bloom, collect healthy flower buds with a size of 2.5-3.0mm. At this time, most of the microspores in the flowe...

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Abstract

The invention discloses a method for increasing the embryogenesis rate of microspores of Wucai, and belongs to the field of vegetable chemical control. The method further comprises the step of spraying brassinolide to seedlings with 6-7 main leaves before obtaining the microspores of the Wucai, the concentration of the brassinolide added to a culture medium is 0.3 mg/L, the concentration of the brassinolide sprayed to the seedlings of the Wucai is 50 mg/L, and the spraying parts of the seedlings of the Wucai are the back surfaces of the leaves until the surfaces of the leaves drip water. The method provided by the invention can effectively improve the occurrence rate of microspore embryos of the Wucai.

Description

technical field [0001] The invention relates to the field of chemical control of vegetables, in particular to a method for improving the embryogenesis rate of aconid microspores. Background technique [0002] Black cabbage is a variety of non-heading Chinese cabbage of the cruciferous family and an important species of Brassica. As an important autumn and winter vegetable, Wucai is cultivated in most parts of our country, especially in the Jianghuai River Basin. Wucai is beautiful in appearance, rich in various vitamins and minerals, and is becoming more and more popular. Wucai is a biennial herbaceous plant. It takes a long time to obtain a pure and inbred line in breeding work, but the use of microspore culture technology can shorten the breeding period and save a lot of time and workload. [0003] The development and environmental adaptation of bracken pollen are affected by a series of processes, among which phytohormone regulation, carbohydrate accumulation and metabo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G22/15A01G7/06
CPCA01H4/005A01H4/001A01G22/15A01G7/06
Inventor 汪承刚汪健朱世东袁凌云赵梦茹侯金锋黄兴学唐小燕张泽根陈国户
Owner ANHUI AGRICULTURAL UNIVERSITY
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