Method for increasing embryogenesis rate of microspores of Wucai
A technology of microspores and occurrence rate, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as hidden dangers, and achieve the effects of safe use, improved embryogenesis rate, and simple operation.
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[0019] 1. Select the full-grained black cabbage variety WS-1 and sow it in the breeding medium. After most of the seeds emerge, perform normal water and fertilizer management. When the seedlings grow to 4-5 true leaves, transplant them into flower pots. The day / night temperature is (25±1)℃ / (18±1)℃, and the photosynthetically active radiation is 300-500μmol·m -2 ·s -1 , The relative humidity is 60-70%, maintain sufficient water and nutrient supply. When the seedlings have 6-7 true leaves, select plants with good growth and no pests and diseases to start spraying with a concentration of 50mg / L BRs. Water was sprayed as a control. The two treatments were sprayed once every 5 days, and the spraying site was the back of the leaves, until the surface dripped, and continued to spray until the early stage of bolting of Chinese cabbage.
[0020] 2. When the plant begins to bloom, collect healthy flower buds with a size of 2.5-3.0mm. At this time, most of the microspores in the flowe...
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