Armeniaca sibirica somatic embryogenesis method

A technology of embryogenesis and cells, applied in the field of plant cultivation, can solve the problems that Siberian apricot tissue culture seedlings have not been applied and research has not been reported, and achieve the effect of low-cost and high-efficiency reproduction

Inactive Publication Date: 2017-05-31
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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Problems solved by technology

But so far, the Siberian apricot tissue culture seedlings have not been used in production, and the research on Siberian apricot body embryogenesis has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Use a brush to remove surface residues of mature Siberian apricot seeds, soak them in 3% potassium permanganate solution for 12 hours, rinse with tap water 3 times, crack the shells in a clean environment, and take out the Siberian apricot kernels. In the ultra-clean workbench, first rinse the seed kernels with sterile water three times, soak them in 75% alcohol for 30 seconds, then wash them with sterile water for 3-5 times, then disinfect them with 2% sodium hypochlorite solution for 3 minutes, and then rinse them with sterile water 3-5 times;

[0018] (2) After the seeds treated in step (1) are peeled off the complete embryos with a scalpel, they are inoculated in the following medium: B5 medium + 6-BA (0.3mg / L) + 2,4-D (0.9mg / L) + KT (0.25mg / L) + sucrose (30g / L) + agar (6g / L), the pH was controlled at 5.8-6.0, the temperature was 23±2°C, and the induction culture was carried out under dark conditions for 35 generate callus;

[0019] (3) Inoculate the callus de...

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Abstract

The invention provides an armeniaca sibirica somatic embryogenesis method, and belongs to a seedling technology in the field of plant cultivation and is a method for inducing somatic embryogenesis with mature armeniaca sibirica embryos. In the method, fully matured armeniaca sibirica seeds are adopted, mature embryos are taken as an explant material, are inoculated on callus induction media for cultivation and are induced to generate an embryonic callus, and the callus are proliferated, so as to achieve the purpose of expanding propagation; further induction is performed by the embryonic callus, so that the embryonic callus is differentiated to form proembryos in the prior period; synchronized cultivation is performed by early proembryos under a certain condition, so that the development of the mature embryos are promoted to realize synchronization; and the early proembryos synchronously cultivated are further matured, so as to form somatic embryos with normal germination conditions. The method has the advantages that a large number of armeniaca sibirica somatic embryos are obtained by adopting the method, so that excellent armeniaca sibirica varieties are proliferated rapidly, and therefore, the propagation coefficient is greatly improved, and the propagation period is shortened.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for Siberian apricot cell embryogenesis. Background technique [0002] Siberian apricot ( Armeniaca sibirica (L.) Lam ) is mainly distributed in the northern region of my country, and is the main economic tree species and ecological greening tree species in the northern region of my country, with wide application and practical value. The common methods of propagation of Siberian apricot are mainly seed propagation and grafting. The grafting method can maintain the excellent characteristics of the variety, and the genetic traits are stable, but the propagation speed through grafting is slow, which seriously hinders the popularization and application of fine varieties in mass production. Stratification and other techniques are required for sowing and propagation. Seed treatment takes a long time and requires high requirements. Moreover, the seed yield is often...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 白玉娥何炎红叶冬梅田有亮林涛桑斯尔珠拉娜苏勒玛乌云塔娜
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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