Armeniaca sibirica somatic embryogenesis method
A technology of embryogenesis and cells, applied in the field of plant cultivation, can solve the problems that Siberian apricot tissue culture seedlings have not been applied and research has not been reported, and achieve the effect of low-cost and high-efficiency reproduction
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[0017] (1) Use a brush to remove surface residues of mature Siberian apricot seeds, soak them in 3% potassium permanganate solution for 12 hours, rinse with tap water 3 times, crack the shells in a clean environment, and take out the Siberian apricot kernels. In the ultra-clean workbench, first rinse the seed kernels with sterile water three times, soak them in 75% alcohol for 30 seconds, then wash them with sterile water for 3-5 times, then disinfect them with 2% sodium hypochlorite solution for 3 minutes, and then rinse them with sterile water 3-5 times;
[0018] (2) After the seeds treated in step (1) are peeled off the complete embryos with a scalpel, they are inoculated in the following medium: B5 medium + 6-BA (0.3mg / L) + 2,4-D (0.9mg / L) + KT (0.25mg / L) + sucrose (30g / L) + agar (6g / L), the pH was controlled at 5.8-6.0, the temperature was 23±2°C, and the induction culture was carried out under dark conditions for 35 generate callus;
[0019] (3) Inoculate the callus de...
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