Method for promoting Chinese cabbage microspore embryogeny and direct seedling development

Abstract

The invention provides a method for promoting Chinese cabbage microspore embryogeny and direct seedling development so as to solve the problems that in an existing dissociative microspore culture technology, the microspore embryogeny rate is low, the direct seedling development rate is low, and the culture period is long. Histone histone deacetylase inhibitor-suberoylanilide hydroxamic acid (SAHA) is added to an induction medium NLN to increase the Chinese cabbage microspore embryogeny rate and the direct seedling development rate. In the NLN induction medium with SAHA of 0.025 micrometer to 0.10 micrometer added, the Chinese cabbage microspore embryogeny rate is increased by 1.42-4.35 times, and the direct seedling development rate is increased by 1.11-1.50 times.

Description

technical field [0001] The invention relates to a method for cultivating free microspores of cabbage, in particular to a method for promoting embryogenesis and direct seedling formation of cabbage microspores. Background technique [0002] Chinese cabbage( brassicarapa L. ssp. chinensis L.) is a typical cross-pollinated plant of Brassicaceae, with significant heterosis. To take advantage of dominant hybrid breeding, the parents must be purified first. The traditional continuous selfing method takes a long time, which is labor-intensive and labor-intensive; it only takes one year to obtain homozygotes using the free microspore culture method, which saves time and saves time. The workload is reduced, and the regenerated plants cultivated in this way can not only create new breeding materials, but also have strong intergenerational stability and strong heterosis. In recent decades, some researchers at home and abroad have done a lot of work on the cultivation of free micros...

AI Technical Summary

Problems solved by technology

To take advantage of dominant hybrid breeding, the parents must be purified first. The traditional continuous selfing method takes a long time, which is labor-intensive and labor-intensive; it only takes one year to obtain homozygotes using the free microspore culture method, which saves time and saves time. The workload is reduced, and the regenerated plants cultivated in this way can not only create new breeding materials, but also have strong intergenerational stability and strong heterosis

In recent decades, some researchers at home and abroad have done a lot of work on the cultivation of free microspores of cruciferous vegetables, and are committed to optimizing the system of microspore embryo formation and embryoid body seedling formation. However, there are still many genotypes of microspore embryogenesis. The low rate of embryoid bodies and the low rate of direct seedling formation limit the application range of this technology in actual breeding work.

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