Method for promoting Chinese cabbage microspore embryogeny and direct seedling development

A technology for embryogenesis and microspores, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as labor-intensive, long time, limited application scope, etc., and achieve the effect of simple and easy operation

Inactive Publication Date: 2015-11-11
SHENYANG AGRI UNIV
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To take advantage of dominant hybrid breeding, the parents must be purified first. The traditional continuous selfing method takes a long time, which is labor-intensive and labor-intensive; it only takes one year to obtain homozygotes using the free microspore culture method, which saves time and saves time. The workload is reduced, and the regenerated plants cultivated in this way can not only create new breeding materials, but also have strong intergenerational stability and strong heterosis
In recent decades, some researchers at home and abroad have done a lot of work on the cultivation of free microspores of cruciferous vegetables, and are committed to optimizing the system of microspore embryo formation and embryoid body seedling formation. However, there are still many genotypes of microspore embryogenesis. The low rate of embryoid bodies and the low rate of direct seedling formation limit the application range of this technology in actual breeding work.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for promoting Chinese cabbage microspore embryogeny and direct seedling development
  • Method for promoting Chinese cabbage microspore embryogeny and direct seedling development
  • Method for promoting Chinese cabbage microspore embryogeny and direct seedling development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The methods described in the following examples are conventional methods unless otherwise specified.

[0019] The reagents described in the following examples can be obtained from commercial sources unless otherwise specified.

[0020] The medium used in the following examples is the same as the B5, NLN, and MS medium in the technical scheme.

[0021] The main process of Chinese cabbage free microspore culture method of the present invention comprises:

[0022] 1. Free microspore culture process

[0023] Select the flower buds whose microspore development period of Chinese cabbage is from the uninucleate marginal stage to the early binucleate stage. At this time, the characteristics of the flower buds are that the ratio of the length of the petals to the length of the anthers is between 1 / 2 and 3 / 4. After washing the flower buds with running water for 2 to 3 times , put the flower buds into a sterilized 100mL beaker, sterilize the surface with 75% ethanol solution for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for promoting Chinese cabbage microspore embryogeny and direct seedling development so as to solve the problems that in an existing dissociative microspore culture technology, the microspore embryogeny rate is low, the direct seedling development rate is low, and the culture period is long. Histone histone deacetylase inhibitor-suberoylanilide hydroxamic acid (SAHA) is added to an induction medium NLN to increase the Chinese cabbage microspore embryogeny rate and the direct seedling development rate. In the NLN induction medium with SAHA of 0.025 micrometer to 0.10 micrometer added, the Chinese cabbage microspore embryogeny rate is increased by 1.42-4.35 times, and the direct seedling development rate is increased by 1.11-1.50 times.

Description

technical field [0001] The invention relates to a method for cultivating free microspores of cabbage, in particular to a method for promoting embryogenesis and direct seedling formation of cabbage microspores. Background technique [0002] Chinese cabbage( brassicarapa L. ssp. chinensis L.) is a typical cross-pollinated plant of Brassicaceae, with significant heterosis. To take advantage of dominant hybrid breeding, the parents must be purified first. The traditional continuous selfing method takes a long time, which is labor-intensive and labor-intensive; it only takes one year to obtain homozygotes using the free microspore culture method, which saves time and saves time. The workload is reduced, and the regenerated plants cultivated in this way can not only create new breeding materials, but also have strong intergenerational stability and strong heterosis. In recent decades, some researchers at home and abroad have done a lot of work on the cultivation of free micros...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 冯辉张露章云唐小燕刘志勇李承彧冀瑞琴王玉刚
Owner SHENYANG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap