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Eucalyptus urophylla*grandis embryoid induction seedling raising method

A technology of embryoid body and Eucalyptus macrophyllum, which is applied in the field of embryoid body induction and embryoid body induction of Eucalyptus macrocaus, can solve the problem of less chimeras in transgenic plants, and achieve stable results and good repeatability

Inactive Publication Date: 2014-01-08
ZHANJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]In summary, there is no report on the embryoid body induction of Eucalyptus urotheca to obtain regenerated plants at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] On the aseptic operation table, select the robust seedless seedlings of Eucalyptus urocarpis, remove the terminal buds and axillary buds, cut the stems into 0.5cm-long segments, and inoculate the stems in the embryogenic callus induction medium (MS Medium + 30g·L -1 Sucrose + 7 g·L -1 Agar + 100 mg·L -1 The volume ratio of Vc+ is 20% coconut milk+0.01 mg·L -1 TDZ+0.1 mg·L -1 NAA, pH 5.8), cultured in dark at 25°C, replaced with fresh medium every 2 weeks, and cultured in dark for 4 weeks;

[0022] After the embryogenic callus was induced, the embryogenic callus was transferred to the embryoid body induction medium (MS medium + 30g·L -1 Sucrose + 7 g·L -1 Agar + 100 mg·L -1 Vc+1 mg·L -1 Spermidine + volume ratio is 20% coconut milk + 0.01 mg·L -1 TDZ+0.05mg·L -1 NAA, pH 5.8), cultured in the dark at 25°C for 3 weeks to obtain embryoid bodies;

[0023] The induced embryoid bodies were transferred to the embryoid body germination medium (MS medium + 30g L -1 S...

Embodiment 2

[0025] On the aseptic operating table, select the robust seedless seedlings of Eucalyptus urocarpitum, remove the terminal buds and axillary buds, cut the stem into 0.6 cm long pieces, and inoculate the stem into embryogenic callus induction medium (MS Medium + 30g·L -1 Sucrose + 7 g·L -1 Agar + 100 mg·L -1 The volume ratio of Vc+ is 20% coconut milk+0.01 mg·L -1 TDZ+0.1 mg·L -1 NAA, pH 5.9), cultured in dark at 25°C, replaced with fresh medium every 2 weeks, and cultured in dark for 5 weeks;

[0026] After the embryogenic callus was induced, the embryogenic callus was transferred to the embryoid body induction medium (MS medium + 30g·L -1 Sucrose + 7 g·L -1 Agar + 100 mg·L -1 Vc+1 mg·L -1 Spermidine + volume ratio is 20% coconut milk + 0.01 mg·L -1 TDZ+0.05mg·L -1 NAA, pH 5.9), cultured in the dark at 25°C for 4 weeks to obtain embryoid bodies;

[0027] The induced embryoid bodies were transferred to the embryoid body germination medium (MS medium + 30g L -1 Sucr...

Embodiment 3

[0029] On the aseptic operating table, select the robust seedless seedlings of Eucalyptus urocarpa, remove the terminal buds and axillary buds, cut the stem into 0.8 cm long pieces, and inoculate the stem into embryogenic callus induction medium (MS Medium + 30g·L -1 Sucrose + 7 g·L -1 Agar + 100 mg·L -1 The volume ratio of Vc+ is 20% coconut milk+0.01 mg·L -1 TDZ+0.1 mg·L -1 NAA, pH 6.0), cultured in dark at 25°C, replaced with fresh medium every 2 weeks, and cultured in dark for 5 weeks;

[0030] After the embryogenic callus was induced, the embryogenic callus was transferred to the embryoid body induction medium (MS medium + 30g·L -1 Sucrose + 7 g·L -1 Agar + 100 mg·L -1 Vc+1 mg·L -1 Spermidine + volume ratio is 20% coconut milk + 0.01 mg·L -1 TDZ+0.05mg·L -1 NAA, pH 6.0), cultured in the dark at 25°C for 3 weeks to obtain embryoid bodies;

[0031] The induced embryoid bodies were transferred to the embryoid body germination medium (MS medium + 30g L -1 Sucrose...

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Abstract

The invention relates to a eucalyptus urophylla*grandis embryoid induction seedling raising method and belongs to the technical field of eucalyptus tissue culture and regeneration. The eucalyptus urophylla*grandis embryoid induction seedling raising method specifically comprises the steps of: taking a eucalyptus urophylla*grandis aseptic seedling stem segment as an explant, and obtaining a complete regenerated plant through embryonic callus induction, embryoid induction and embryoid germination. The eucalyptus urophylla*grandis embryoid induction seedling raising method can be adopted for obtaining the regenerated plant by an embryogenesis way with stable results and good repeatability, can be applied in the production and large-scale commercialization of seedlings, and also lays a foundation for eucalyptus urophylla*grandis breeding.

Description

technical field [0001] The invention relates to a method for inducing embryoid bodies, in particular to a method for inducing embryoid bodies of Eucalyptus urophylla and belonging to the technical field of tissue culture regeneration of Eucalyptus eucalyptus. Background technique [0002] Eucalyptus is native to Australia and belongs to the genus Eucalyptus (Myrtaceae) Eucalyptus ) collectively referred to as plants, there are 1039 species, subspecies and varieties. Eucalyptus has been introduced to nearly 100 countries and regions in the world due to its fast growth, short rotation period, drought resistance, thinness resistance, wide adaptability, wide range of uses, and high economic benefits. It is distributed in the Mediterranean region, Southeast Asia, and America. And Africa, has become one of the three major plantation tree species recognized in the world at present. According to FAO statistics, eucalyptus plantations account for 10% of the world's total plantation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 欧阳乐军曾富华黄真池沙月娥陈家清陈丽云
Owner ZHANJIANG NORMAL UNIV
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