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Bionic eyelid scaffold having double-layer composite structure and construction method thereof

A double-layer composite, eyelid technology, applied in the field of bionic eyelid stent and its preparation, can solve the problems of different antigenicity, difficult to adhere well to the ocular surface, non-biodegradable, etc., and achieves good biocompatibility and good biocompatibility. Application prospect, simple preparation method effect

Inactive Publication Date: 2020-02-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Allogeneic / xenogeneic tissue materials are relatively easy to obtain and easy to operate. For example, autologous hard palate mucosa can achieve a good repair effect after being implanted as the posterior eyelid layer, but its keratinized mucosal epithelium will cause corneal damage in upper eyelid repair; allogeneic / Heterogeneous cartilage has higher hardness and better support strength, but poor toughness, so it is difficult to attach well to the ocular surface
In addition, due to the difference in tissue antigenicity between the allogeneic / xenograft and the body, it is impossible to completely avoid the risk of immune rejection and transmission of other potential infectious diseases after implantation, and the material is easily degraded and absorbed
On the other hand, compared with natural tissue materials, synthetic materials are more prone to rejection and infection, have relatively poor histocompatibility, are not biodegradable, and require conjunctival reconstruction at the same time, so their clinical application in eyelid reconstruction is limited. certain limit

Method used

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  • Bionic eyelid scaffold having double-layer composite structure and construction method thereof
  • Bionic eyelid scaffold having double-layer composite structure and construction method thereof
  • Bionic eyelid scaffold having double-layer composite structure and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Add gelatin particles with a thickness of 1mm and a particle size of 180-280μm into the polyethylene template, then inject an ethanol solution with a volume concentration of 85%, put it in an oven at a temperature of 40°C, and wait until the solution is completely dried to form gelatin particles After bonding, a gelatin template sheet with a thickness of 1 mm was taken out to form a gelatin template.

[0037] Then a blend of polytrimethylene fumarate (PPF) and hydroxyethyl methacrylate (HEMA) at a mass ratio of 1:2 (containing 1% benzoyl peroxide as an initiator) was used as a degradable elastic polyester The blend was introduced into a gelatin template, cross-linked at 85°C for 4 hours, then soaked in a constant temperature water bath at 37°C, and the gelatin template was removed to obtain porous polytrimethylene fumarate / hydroxyethyl methacrylate The elastic support is a porous elastic polyester support.

[0038] 2) Using the porous elastic polyester scaffold prep...

Embodiment 2

[0042] The preparation method is the same as that in Example 1, the difference is that the 0.5% type I collagen solution in step 2) is replaced with a 40 mg / mL fibrinogen solution, and thrombin with a working concentration of 25 U / mL is added, and the mixture is exchanged at a temperature of 37°C. Combined for 1 hour, freeze-dried, and the smaller surface pores were obtained after removal, and the denser polytrimethylene fumarate / fibrin glue double-layer scaffold was obtained.

Embodiment 3

[0044] The preparation method is the same as in Example 1, the difference is that the particle size of the gelatin particles in step 1 is changed to 280-450 μm, and the freezing at -20°C for 2 hours in step 2 is changed to -80°C for 1 hour, by changing the size of the porogen , the freezing temperature to regulate the pore size and porosity of the scaffold.

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Abstract

The invention discloses a bionic eyelid scaffold having a double-layer composite structure and a construction method thereof. The bionic eyelid scaffold is mainly formed by compounding of two layers of different bionic eyelid tissue materials; a bottom layer is a porous degradable elastic polyester skeleton, and a surface layer is a natural biological material, that is a sponge; and porous degradable elastic polyester is taken as a raw material, an eyelid skeleton material having a communicating pore is prepared by using a gelatin template method, and the natural biological material is composited to the eyelid skeleton material, thus obtaining the bionic eyelid scaffold having the double-layer composite structure in tissue engineering. The elastic polyester / natural biological material composite scaffold prepared by the construction method of the invention has a matched mechanical strength and maintains an eyelid form, and simultaneously has a good biocompatibility and can promote growth and functional maintenance of fibrocytes and conjunctival cells; and the preparation method is simple, and the repeatability is good.

Description

technical field [0001] The invention relates to a layered support carrier for interface tissue engineering and a construction method thereof, in particular to a bionic eyelid support composed of elastic polyester and natural biological materials and a preparation method thereof. Background technique [0002] Interface tissue engineering (ITE) is an emerging field to repair or regenerate diseased or damaged regional tissue types between different regions with the aim of regenerating functional tissue. Tissue engineering typically uses conventional biomaterials to engineer individual tissues (i.e., noninterfacial tissues), such as skin, cartilage, bone, and nerves, rather than tissue interfaces. Indeed, interfacial tissues in the body consist of complex structures and properties that may not be regenerated by using conventional scaffolds made of single-phase or isotropic biomaterials. [0003] The eyelid is a functional structure located on the surface of the eyeball. It can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/24A61L27/22A61L27/20A61L27/50A61L27/56A61L27/58
CPCA61L27/18A61L27/20A61L27/222A61L27/225A61L27/24A61L27/50A61L27/56A61L27/58A61L2430/16C08L67/02C08L5/02
Inventor 高长有叶娟徐佩芳高琪
Owner ZHEJIANG UNIV
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