Preparation method and application of silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold

A silk fibroin and nanofiber technology, applied in fiber treatment, tissue regeneration, electrospinning, etc., to achieve high water content, promote tissue regeneration, and facilitate adhesion

Active Publication Date: 2019-07-12
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although there are many studies and patents on cartilage biomimetic scaffolds and silk fibroin hydrogels, there is still no short nanofibers made from blended nanofiber membranes obtained by electrospinning, mixed with silk fibroin solution, and spongy cartilage biomimetic scaffolds. report

Method used

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  • Preparation method and application of silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold
  • Preparation method and application of silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold
  • Preparation method and application of silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold

Examples

Experimental program
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Embodiment 1

[0038] (1) Obtain regenerated silk fibroin from silkworm cocoons, purify industrial grade chitin (Aladdin), weigh regenerated silk fibroin and purified chitin according to the ratio of silk fibroin: purified chitin = 6:1 , respectively dissolve the two in the organic solvent HFIP, stir until clear and transparent, mix the two solutions, and keep the final concentration of silk fibroin at 6% (w / w).

[0039] (2) The mixed solution is electrospun to obtain blended nanofibers. The electrospinning conditions are voltage 16kV, flow rate 0.8mL / h, distance between the aluminum foil plate receiver and the needle head 15cm, relative temperature and relative humidity are 25°C and 50% respectively , the resulting blended nanofiber membrane was dried in a vacuum oven for 3 days to remove residual HFIP, and the field emission scanning electron microscope image of the nanofiber membrane was as follows figure 2 A&D shown. The water insolubility is increased after ethanol gradient cross-link...

Embodiment 2

[0045] (1). Regenerated silk fibroin is obtained from silkworm cocoons, and industrial-grade chitin (Aladdin) is purified, and silk fibroin and purified chitin are weighed according to the ratio of silk fibroin:purified chitin=7:1 , respectively dissolve the two solutions in the organic solvent HFIP, stir until clear and transparent, mix the two solutions, and keep the final concentration of silk fibroin at 7% (w / w).

[0046] (2). The mixed solution is electrospun to obtain blended nanofibers. The electrospinning conditions are voltage 16kV, flow rate 0.8mL / h, distance between the aluminum foil plate receiver and the needle head 15cm, relative temperature and relative humidity respectively 25 ℃ and 50 %, the resulting blended nanofiber membrane was dried in a vacuum oven for 3 days to remove residual HFIP, and the field emission scanning electron microscope image of the nanofiber membrane is shown in figure 2 Shown by B&E. The water insolubility is increased after ethanol gr...

Embodiment 3

[0050] (1). Obtain regenerated silk fibroin from silkworm cocoons, purify industrial grade chitin (Aladdin), weigh silk fibroin and purified chitin according to the ratio of silk fibroin:purified chitin=8:1 , respectively dissolve the two solutions in the organic solvent HFIP, stir until clear and transparent, mix the two solutions, and keep the final concentration of silk fibroin at 8% (w / w).

[0051] (2). The mixed solution is electrospun to obtain blended nanofibers. The electrospinning conditions are voltage 16kV, flow rate 0.8mL / h, distance between the aluminum foil plate receiver and the needle head 15cm, relative temperature and relative humidity respectively 25 ℃ and 50 %, the resulting blended nanofiber membrane was dried in a vacuum oven for 3 days to remove residual HFIP, and the field emission scanning electron microscope image of the nanofiber membrane is shown in figure 2 C&F shown. The water insolubility is increased after ethanol gradient cross-linking, the c...

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Abstract

The invention discloses a preparation method and application of a silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold. The method utilizes mulberry silk to prepare regenerated the silk fibroin, purifies industrial grade chitin, mixes a silk fibroin solution and a chitin solution, prepares a blended nanofiber membrane by an electrospinning technique, cross-links with ethanol to form a short fiber through a blended nanofiber, mixes the short fiber and the silk fibroin solution, conducts cross-linking by a crosslinking agent, and conducts freeze-forming to obtain a spongy cartilage bionic scaffold. In particular, the insertion of the nano short fiber increases the compressive strength and biocompatibility of the scaffold material. The method constructs the biomimetic scaffold with good biocompatibility, low immune rejection and similar composition with natural cartilage matrix, has wide material source, low cost and simple preparation process, can beused for constructing tissue engineering cartilage and repairing cartilage regression, and has good clinical application prospects.

Description

technical field [0001] The invention relates to the technical field of scaffold preparation for cartilage tissue engineering, in particular to a preparation method of a bionic scaffold based on silk fibroin and chitin blended nanofiber embedded hydrogel cartilage. Background technique [0002] Osteochondral defects caused by trauma, tumors, and various arthritis diseases have brought great pain and heavy economic burden to patients and their families, seriously affecting the quality of life of patients. At present, subchondral bone microfractures, autologous or allogeneic osteochondral transplantation, and xenogeneic osteochondral transplantation are generally used in the clinical treatment of articular osteochondral defects. Cartilage transplantation will cause new trauma, which is easy to cause complications, and heterogeneous osteochondral transplantation is also easy to cause disease transmission. Therefore, they cannot be widely used. [0003] In recent years, with th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/52A61L27/50A61L27/54A61L27/26D04H1/728D01D5/00
CPCA61L27/26A61L27/50A61L27/52A61L27/54A61L27/56A61L2300/252A61L2300/41A61L2300/412A61L2430/02D01D5/003D04H1/728C08L89/00C08L5/08
Inventor 邓红兵戴金虹李丹施晓文杜予民
Owner WUHAN UNIV
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