Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for constructing in-vitro tissue engineering cartilage

A technology of tissue engineering and constructs, applied in the field of bionic scaffolds, can solve the problems of loss of cartilage phenotype, hypertrophy or calcification of chondrocytes, a large amount of fibrotic cartilage, etc., and achieve the effect of promoting phenotype maintenance and growth of chondrocytes

Active Publication Date: 2021-06-01
DONGHUA UNIV +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chondrocytes induced or cultured by such conditions are prone to hypertrophy or calcification, and are also prone to dedifferentiation and loss of cartilage phenotype, and even a large amount of fibrotic cartilage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing in-vitro tissue engineering cartilage
  • Method for constructing in-vitro tissue engineering cartilage
  • Method for constructing in-vitro tissue engineering cartilage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for constructing tissue engineered cartilage in vitro, the specific steps are:

[0035] (1) Preparation of cartilage biomimetic scaffold;

[0036] (1.1) Preparation of raw materials;

[0037] The average diameter is 130nm and the material is the nanofiber bundle of bacterial cellulose; The silk fibroin aqueous solution with the concentration of 6wt%; The H concentration of 490mM 2 o 2 Aqueous solution; HRP solution with a concentration of 1000U / ml (HRP is dissolved in PBS buffer to obtain);

[0038] (1.2) Mix the nanofiber bundles and the silk fibroin aqueous solution to obtain a mixed solution; in the mixed solution, the weight ratio of the silk fibroin and the nanofiber bundles is 95:5;

[0039] (1.3) Add H to the mixture in sequence 2 o 2 Aqueous solution and HRP solution form a crosslinking system; H 2 o 2 The addition of the aqueous solution accounts for 5vol% of the volume content of the crosslinking system; the addition of the HRP solution account...

Embodiment 2

[0054] A method for constructing tissue engineered cartilage in vitro, the specific steps are:

[0055] (1) Preparation of cartilage biomimetic scaffold;

[0056] (1.1) Preparation of raw materials;

[0057] Nanofiber bundles with an average diameter of 30nm and a material made of polyvinyl alcohol; a concentration of 5wt% elastin in water; a concentration of 490mM H 2 o 2 Aqueous solution; HRP solution with a concentration of 1000U / ml (HRP is dissolved in PBS buffer to obtain);

[0058] (1.2) Mix the nanofiber bundles with the elastin aqueous solution to obtain a mixed solution; in the mixed solution, the weight ratio of the elastin and the nanofiber bundles is 95:5;

[0059] (1.3) Add H to the mixture in turn 2 o 2 Aqueous solution and HRP solution form a crosslinking system; H 2 o 2 The addition of the aqueous solution accounts for 3vol% of the volume content of the crosslinking system; the addition of the HRP solution accounts for 4vol% of the volume content of the ...

Embodiment 3

[0069] A method for constructing tissue engineered cartilage in vitro, the specific steps are:

[0070] (1) Preparation of cartilage biomimetic scaffold;

[0071] (1.1) Preparation of raw materials;

[0072] Nanofiber bundles with an average diameter of 100nm and made of polylactic acid; a gelatin aqueous solution with a concentration of 12wt%; a concentration of 490mM H 2 o 2 Aqueous solution; HRP solution with a concentration of 1000U / ml (HRP is dissolved in PBS buffer to obtain);

[0073] (1.2) Mix the nanofiber bundles with the gelatin aqueous solution to obtain a mixed solution; in the mixed solution, the weight ratio of the gelatin and the nanofiber bundles is 95:5;

[0074] (1.3) Add H to the mixture in sequence 2 o 2 Aqueous solution and HRP solution form a crosslinking system; H 2 o 2 The addition of the aqueous solution accounts for 6vol% of the volume content of the crosslinking system; the addition of the HRP solution accounts for 5vol% of the volume content...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Apertureaaaaaaaaaa
Compressive strengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for constructing in-vitro tissue engineering cartilage, and the method comprises the following steps: inoculating cartilage cells onto a cartilage bionic scaffold, and applying stepped dynamic hydrostatic pressure to stimulate culture after adhesion, wherein the average pore size of the cartilage bionic scaffold is more than 100 microns, the stepped dynamic hydrostatic pressure means that the hydrostatic pressure changes in a stepped mode along with time, the number of steps is two or more, the hydrostatic pressure corresponding to each step is selected within the range of 0.1 MPa to 10 MPa, the duration time of the hydrostatic pressure corresponding to each step is selected within the range of 10 s to 100 s, and the difference between the hydrostatic pressures corresponding to the adjacent steps is 1.5 times or above. The in-vitro tissue engineering cartilage with good cartilage phenotype maintenance is constructed by combining construction of a proper natural protein cartilage bionic scaffold and stepped circulating dynamic hydrostatic pressure stimulation culture, and the mechanical property, porosity and other characteristics of the scaffold material are all suitable for adhesion growth and phenotype maintenance of cartilage cells; the stepped circulating dynamic hydrostatic pressure stimulation shows excellent cartilage cell growth promotion and phenotype maintenance capabilities.

Description

technical field [0001] The invention belongs to the technical field of bionic scaffolds, and relates to a method for constructing tissue engineered cartilage in vitro, and more particularly relates to a method for constructing tissue engineered cartilage in vitro based on cartilage bionic scaffolds in cooperation with dynamic hydrostatic pressure culture. Background technique [0002] Due to the lack of blood vessels, lymph and nerves in the cartilage, it is difficult to repair itself once it is damaged. Over time, diseases such as osteoarthritis will follow. Currently, there is still a lack of an effective strategy to repair or reverse damaged cartilage. In recent years, tissue engineering technology has become an important development direction of cartilage repair. Among the many seed cells for cartilage tissue engineering, autologous chondrocytes are the most widely used and studied in clinical and academic circles. Combining seed cells, scaffold materials and cytokines...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/38A61L27/16A61L27/18A61L27/20A61L27/22A61L27/56A61L27/58A61F2/30
CPCA61L27/3817A61L27/3852A61L27/20A61L27/18A61L27/16A61L27/227A61L27/225A61L27/222A61L27/58A61L27/56A61F2/30756A61F2002/2835A61L2400/12A61L2430/06
Inventor 姚响张耀鹏谷敏婧范苏娜
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com