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Design of cancellous bone bionic scaffold prepared by 3d printing technology and application thereof

A 3D printing and bionic technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as non-union, application limitations, and easy infection, and achieve the effect of eliminating variable differences and reliable scaffold models

Inactive Publication Date: 2019-12-13
广州溯原生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited source of autologous bone, a second operation area needs to be opened for bone harvesting to cause new bone defects, which is prone to complications such as infection, hematoma, and nerve injury, and secondary damage and new bone defects are prone to occur during the repair process. As a result, its clinical application is limited, and autologous bone grafting cannot meet the huge clinical needs.
Compared with autologous bone, allogeneic bone comes from a wider range of sources, and is usually processed to reduce antigenicity and facilitate storage. However, even so, some patients still experience allogeneic bone rejection, resorption, and non-union

Method used

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  • Design of cancellous bone bionic scaffold prepared by 3d printing technology and application thereof
  • Design of cancellous bone bionic scaffold prepared by 3d printing technology and application thereof
  • Design of cancellous bone bionic scaffold prepared by 3d printing technology and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Design and preparation of cancellous bone bionic scaffold

[0031] 1.1 Three-dimensional modeling of cancellous bone biomimetic scaffold

[0032]Rhinoceros is a commonly used parametric 3D model design software. In this study, through the Rhinoceros 5 software (v.5.1.30103.145) and the Grasshopper plug-in (v.August-27, 2014) algorithm function design loose Bone biomimetic model.

[0033] The bionic bracket designed in this study mainly uses the three-dimensional Voronoi algorithm. The Voronoi diagram is also called the Thiessen polygon. In a single plane, a vertical line is drawn at the midpoint of the line connecting two adjacent points. These vertical lines divide each point. into an independent region. If the Voronoi algorithm is applied to a three-dimensional space, a vertical plane is made at the midpoint of the line connecting two adjacent points, and these vertical planes enclose each point into an independent space. The Voronoi algorithm is used to ...

Embodiment 2

[0069] Example 2 Calcination of natural bovine cancellous bone by ammonium dihydrogen phosphate method and detection of scaffold structure

[0070]2.1 Calcination of bovine cancellous bone by ammonium dihydrogen phosphate method

[0071] 2.1.1 Bone preparation and pretreatment

[0072] Fresh adult femurs were purchased from the local market. Use a hacksaw to cut off the femoral neck and medial and lateral condyles from both ends of the femur. Soak them in 0.5M / L sodium hydroxide for 3 hours at room temperature and pressure, boil them for 30 minutes, and cut them with a hacksaw. Saw several cancellous bone pieces with a size of 10×10×10 mm, soak them in 0.5M / L sodium hydroxide for 24 hours, and dry them.

[0073] 2.1.2 Calcined bone by ammonium dihydrogen phosphate method

[0074] Place the bone in a muffle furnace, slowly raise the temperature (6°C / min) to 900°C, keep it for 4 hours, and take it out after cooling. Soak in 0.5M ammonium dihydrogen phosphate for 24 hours, dry...

Embodiment 3

[0100] Example 3 In Vitro Biological Evaluation

[0101] 3.1 Extraction and culture of rat bone marrow mesenchymal stem cells

[0102] One 4-week-old male SD rat was selected and sacrificed by cervical dislocation, soaked in 0.5% iodophor for 3 minutes for disinfection, and then separated the bilateral femur and tibia under aseptic conditions, washed out the bone marrow with low-sugar DMEM culture medium, and obtained cell suspension Centrifuge at 2000r / min for 10min. Then resuspend with 15mL DMEM medium containing 10% fetal bovine serum and 1% double antibody, and add 1×10 8 L -1 Density seeded at 25cm 2 Place in a culture bottle and culture in a 37°C, CO2 constant temperature incubator. 24 hours after inoculation, half of the medium was changed for the first time to remove unattached cells. Afterwards, the medium was changed every 3 days. When the adherent cells grew and reached more than 90% confluence, they were digested and subcultured with 0.25% trypsin, and subcul...

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Abstract

The invention relates to a novel bionic scaffold model constructed through a 3D-Voronoi algorithm and a random distribution micropore algorithm, and a high-precision tissue engineering scaffold is manufactured by using a biological 3D printing technology. The bovine femur is calcined by using an ammonium dihydrogen phosphate secondary calcination method, inorganic components such as hydroxyapatiteand the like are removed on the basis of realizing allogeneic bone degreasing, decalcification and antigen removal, so that the remainder is high-purity beta-tricalcium phosphate, and variable difference caused by a matrix material in an experiment is eliminated while the form of a natural cancellous bone is retained. Through various experiments such as characterization detection, structural analysis, in-vitro experiments and in-vivo implantation, the influence of different scaffold structures on cell proliferation and adhesion and in-vivo bone repair effects is comprehensively evaluated, a reliable scaffold model and an effective detection means are provided for bionic structure design of the bone tissue engineering scaffold, and the bone tissue engineering scaffold can be used for clinically treating various bone defects.

Description

technical field [0001] The invention belongs to the field of tissue engineering, more specifically, the invention relates to the application of a novel bionic bracket model. Background technique [0002] The skeletal system of the human body has important functions such as maintaining movement, protecting internal organs, and supporting body weight. In my country, there are many cases of bone defects caused by trauma, infection, tumor, surgical resection and other reasons every year. The repair of bone defects is facing a huge clinical demand, and it has been showing a rising trend. There are many repair materials for bone defects, which can be divided into autologous materials, allogeneic materials and artificial materials according to the source. [0003] Autologous bone grafting is the gold standard for the treatment of bone defects. It has the advantages of no immune rejection, complete absorption, and effective induction of bone reconstruction. At present, a small am...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/12A61L27/56A61L27/50B33Y10/00B33Y70/00
CPCA61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/3834A61L27/3847A61L27/12A61L27/56A61L27/50B33Y10/00B33Y70/00A61L2430/02A61L2300/412A61L2430/40
Inventor 孙晗笑利时雨
Owner 广州溯原生物科技股份有限公司
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