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Silver nanowire-mineralized collagen co-assembled biomimetic scaffold and its preparation method and application

A technology of silver nanowires and mineralized collagen, which is applied in the field of biomedicine, can solve the problems of inability to regenerate bone tissue and prone to infection, reduce biological toxicity, avoid affecting compatibility and osteogenic performance, and the preparation process is simple and easy line effect

Active Publication Date: 2021-10-01
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing artificial bone materials only have a limited physical support effect, and cannot truly realize the regeneration of bone tissue structure and function, especially the arrangement of collagen and blood vessel formation; at the same time, the maxillofacial area is a bacterial environment, and bone defects are prone to occur Infection, therefore, improving the antibacterial properties of bone substitute materials is the key to optimize the effect of bone defect repair

Method used

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  • Silver nanowire-mineralized collagen co-assembled biomimetic scaffold and its preparation method and application
  • Silver nanowire-mineralized collagen co-assembled biomimetic scaffold and its preparation method and application
  • Silver nanowire-mineralized collagen co-assembled biomimetic scaffold and its preparation method and application

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Embodiment 1

[0036] A method for preparing a silver nanowire-mineralized collagen co-assembled biomimetic scaffold material, the specific steps are as follows:

[0037] (1) Preparation of silver nanowires (AgNW): Weigh 0.334g of PVP powder into a three-necked flask, add 17mL of ethylene glycol, dissolve with magnetic stirring and heat to 170°C; weigh 0.025g of silver chloride powder and put In 1mL of ethylene glycol; quickly inject the ethylene glycol solution of silver chloride into the PVP solution at 170°C; after 5 minutes, weigh the previously weighed 0.110g of silver nitrate in ethylene glycol solution, and inject it within 10 minutes In a three-necked flask, continue to react at 170°C for 30 minutes. Continuous magnetic stirring is required during the entire reaction process; Repeat the centrifugation once, then centrifuge at 1000r / min for 15min and repeat three times to obtain a silver nanowire (AgNW) solution; the centrifuged AgNWs are dispersed in water for subsequent characterizati...

Embodiment 2

[0044] The method for preparing a silver nanowire-mineralized collagen co-assembled biomimetic scaffold material; the specific steps are as follows:

[0045] (1) Preparation of silver nanowires (AgNW): Weigh 0.334g of PVP powder into a three-necked flask, add 17mL of ethylene glycol, dissolve with magnetic stirring and heat to 170°C; weigh 0.025g of silver chloride powder and put in 1 mL of ethylene glycol. Quickly inject the ethylene glycol solution of silver chloride into the PVP solution at 170°C; after 5 minutes, weigh the previously weighed 0.110g of silver nitrate in ethylene glycol solution, and inject it into the three-neck flask within 10 minutes, at 170°C Continue the reaction for 30 minutes, and continuous magnetic stirring is required throughout the reaction process; after the reaction is completed, wait for the solution to cool down to room temperature naturally, add appropriate amount of deionized water and ethanol, centrifuge at 8000r / min for 5min and repeat the...

Embodiment 3

[0052] The preparation method of silver nanowire-mineralized collagen co-assembled biomimetic scaffold material, the steps are as follows:

[0053] (1) Preparation of silver nanowires (AgNW): heat the PVP solution, stir at a constant temperature, add silver chloride solution to react; then add silver nitrate solution to react; cool to room temperature, add deionized water and ethanol mixed solution, and centrifuge. Obtain silver nanowire (AgNW) solution;

[0054] (2) Silver nanowire-collagen co-assembly: using thermodynamically controlled bottom-up self-assembly mode, the extracted and purified type I rat tail collagen solution was concentrated with PEG, and the collagen solution was prepared with step (1). The obtained silver nanowire (AgNW) solution was mixed and dialyzed to obtain a co-assembled silver nanowire-collagen;

[0055] (3) Synthesis of a three-dimensional porous scaffold: the co-assembled silver nanowire-collagen obtained in step (2) was centrifuged, stirred int...

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Abstract

The present invention provides a silver nanowire-mineralized collagen co-assembled biomimetic scaffold material, the silver nanowire-mineralized collagen co-assembled biomimetic scaffold material is a three-dimensional porous scaffold, and the three-dimensional porous scaffold is composed of silver nanowires, Collagen fibers wrapped on the surface of silver nanowires (AgNW) are interwoven, and nano-hydroxyapatite filled in the collagen fibers is also included. The present invention also provides a preparation method for the above-mentioned silver nanowire-mineralized collagen co-assembled biomimetic scaffold material. Collagen molecules wrapped on the surface of silver nanowires in the silver nanowire-mineralized collagen co-assembled biomimetic scaffold of the present invention can reduce the biotoxicity of the material and avoid affecting the compatibility and osteogenic performance; when the surface collagen molecules degrade in vivo, the partially exposed Silver nanowires can effectively inhibit the survival of bacteria at the implant site, and realize bone repair / antibacterial dual-mode treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a silver nanowire-mineralized collagen co-assembled biomimetic scaffold (IMC / AgNWs) and a preparation method thereof, and also provides the silver nanowire-mineralized collagen co-assembled biomimetic scaffold material in the preparation of treatment and / or prevent the application of infectious bone defect repair drugs. Background technique [0002] Maxillofacial bone defects caused by maxillofacial infectious diseases such as periodontitis, peri-implantitis, and osteomyelitis of the jaw are a major problem in clinical oral treatment. At present, the use of artificial bone substitute materials is an effective solution for the treatment of bone defects. However, the existing artificial bone materials only have a limited physical support effect, and cannot truly realize the regeneration of bone tissue structure and function, especially the arrangement of collagen and blood vessel form...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/04A61L27/12A61L27/24A61L27/50A61L27/54A61L27/56A61L27/58B22F9/24B82Y40/00
CPCA61L27/047A61L27/12A61L27/24A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/104A61L2300/404A61L2430/02B22F9/24B82Y40/00
Inventor 刘燕罗聃李紫昕
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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