Myocardial biomimetic scaffold of composite conductive material and preparation method thereof

A composite conductive and bionic technology, applied in medical science, prosthesis, etc., can solve the problem of inability to proliferate, and achieve the effect of promoting the growth of cardiomyocytes and the formation of myocardial lamellae, increasing the elongation rate, and promoting the growth of cardiomyocytes.

Active Publication Date: 2017-05-17
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shin et al. incorporated graphene oxide into gelatin-methyl methacrylate graft copolymer and used 3T3 cells to study its cytocompatibility, but 3T3 cells are fibroblast cell lines with strong proliferation ability and sustainable passage, while Cardiomyocytes are primary cells, unable to proliferate, and their characteristics are very different from 3T3 cells

Method used

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  • Myocardial biomimetic scaffold of composite conductive material and preparation method thereof
  • Myocardial biomimetic scaffold of composite conductive material and preparation method thereof
  • Myocardial biomimetic scaffold of composite conductive material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1. Preparation of graphene oxide-gelatin solution: Weigh 0.6g of gelatin and dissolve it in 10mL of 0.3mg / mL graphene oxide solution. After swelling at room temperature for 30min, dissolve in a water bath at 40°C to form gelatin. Mass volume ratio is 6% graphene oxide-gelatin solution;

[0027] Step 2. Preparation of graphene oxide-gelatin hydrogel scaffold without microstructure: draw 400 μL of the graphene oxide-gelatin solution in step 1 and inject it into a cylindrical mold with an inner diameter of 10 mm, and place it in an environment of 4°C for 2 hours. A microstructure-free graphene oxide-gelatin hydrogel scaffold can be formed.

[0028] Step 3, genipin cross-linked hydrogel: weigh a certain amount of genipin and dissolve it in ultrapure water to form a genipin solution with a mass volume ratio of 0.1%. The graphene oxide-gelatin hydrogel scaffold prepared above was soaked in genipin solution, and placed in a 5°C incubator for cross-linking for 4h. After ...

Embodiment 2

[0030] Step 1. Preparation of graphene oxide-gelatin solution: Weigh 0.6g of gelatin and dissolve it in 10mL of 0.6mg / mL graphene oxide solution. After swelling at room temperature for 30min, dissolve it in a water bath at 60°C to form gelatin. Mass volume ratio is 6% graphene oxide-gelatin solution;

[0031] Step 2. Preparation of graphene oxide-gelatin hydrogel scaffold without microstructure: draw 400 μL of the graphene oxide-gelatin solution in step 1 and inject it into a cylindrical mold with an inner diameter of 10 mm, and place it in an environment of 10°C for 2 hours. A microstructure-free graphene oxide-gelatin hydrogel scaffold can be formed.

[0032] Step 3, genipin cross-linked hydrogel: Weigh a certain amount of genipin and dissolve it in HEPES buffer (10 mM, pH 7.4) to form a genipin solution with a mass volume ratio of 0.2%. The hydrogel scaffold prepared above was soaked in the genipin solution, and placed in an incubator at 25°C for cross-linking for 24 hours...

Embodiment 3

[0034] Step 1. Preparation of graphene oxide-gelatin solution: Weigh 0.6g of gelatin and dissolve it in 10mL of 0.6mg / mL graphene oxide solution. After swelling at room temperature for 30min, dissolve it in a water bath at 60°C to form gelatin. Mass volume ratio is 6% graphene oxide-gelatin solution;

[0035]Step 2. Preparation of graphene oxide-gelatin hydrogel scaffold without microstructure: draw 400 μL of the graphene oxide-gelatin solution in step 1 and inject it into a cylindrical mold with an inner diameter of 10 mm, and place it in an environment of 4°C for 2 hours. A microstructure-free graphene oxide-gelatin hydrogel scaffold can be formed.

[0036] Step 3, genipin cross-linked hydrogel: Weigh a certain amount of genipin and dissolve it in HEPES buffer (10 mM, pH 7.4) to form a genipin solution with a mass volume ratio of 0.8%. The hydrogel scaffold prepared above was soaked in the genipin solution, and placed in an incubator at 25°C for cross-linking for 24 hours. ...

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Abstract

The invention discloses a myocardial bionic scaffold made from a composite conducting material and a preparation method thereof. According to the preparation method disclosed by the invention, a graphene oxide-gelatin hydrogel bionic scaffold with a reinforced conducting performance is formed by compounding a conducting material graphene oxide with gelatine, and then crosslinking genipin without biotoxicity. The elasticity modulus of the bionic scaffold can be regulated and controlled by changing a crosslinking degree, and the mechanical properties of internal myocardium can be better simulated; meanwhile, the surface micro-structure of the scaffold is controllable and the conducting material, that is, graphene oxide is compounded, thus realizing simulation for an internal myocardium micro-environment, promoting the growth of myocardial cells, and forming a highly-ordered space structure and conduction for intercellular electrophysiological signals and contraction signals; and therefore, the growth of the myocardial cells can be promoted and the functions of the myocardial cells can be improved.

Description

technical field [0001] The invention belongs to the field of tissue engineering, in particular to a gelatin hydrogel myocardial bionic scaffold compounded with graphene oxide and a preparation method thereof. Background technique [0002] Cardiovascular disease induces a variety of serious complications, such as myocardial infarction, which leads to myocardial necrosis and loss and death of myocardial cells. It ranks first in the global mortality rate and seriously affects and endangers human health and life. The current treatment methods for heart disease are mainly heart transplantation and installation of left ventricular assist devices (LVADs). However, heart transplantation faces the problem of limited donor organs and the high cost of LVADs. The purpose of myocardial tissue engineering is to repair damaged myocardium under the guidance of scaffolds that can simulate in vivo myocardial tissue, cells and / or biomaterials, or to screen drugs in vitro to exclude drugs that...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/08A61L27/52A61L27/50
Inventor 黄宁平刘海霞
Owner SOUTHEAST UNIV
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