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Injectable extracellular cartilage matrix particle and application of particle to implants

A decellularized cartilage and implant technology, applied in the field of regenerative medicine, can solve the problems of large particle size, weakened cosmetic repair effect, injection, etc., achieve good biocompatibility, good cosmetic repair effect, and reduce immune rejection Effect

Inactive Publication Date: 2017-03-15
广州昕生医学材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method for preparing microparticles in this patent is only to physically cut the crosslinked material into microparticles with a side length of 300-500 μm. However, the particle size is too large to be injected with a needle with a smaller diameter; Practical value in the field of plastic surgery
[0005] Chinese patent CN02156797.2 discloses a preparation method of injectable collagen and its products and applications. This method uses collagen fibers in animal tissues to prepare injectable collagen. However, the original animal tissue will be destroyed during the preparation process. The structure and other components also lose the active components with biological activity or biological function in the tissue, and can only play a role of pure collagen filling
[0006] As mentioned above, there are some shortcomings in the cosmetic plastic implant materials currently on the market and the preparation methods of the cosmetic plastic implant materials disclosed in similar domestic patents, especially collagen and hyaluronic acid implant products, which do not contain bioactive factors. , so its function is only filling, and the cosmetic effect of this type of implant product is temporary. When these filling materials are gradually decomposed and absorbed by the body, the cosmetic repair effect will weaken or disappear
At present, there is no product that uses acellular cartilage as a raw material to prepare injectable filling materials, and there are no patents or literature reports

Method used

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  • Injectable extracellular cartilage matrix particle and application of particle to implants
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Experimental program
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Effect test

Embodiment 1

[0048] The preparation method of injectable decellularized cartilage matrix microparticles comprises the following steps:

[0049] 1) Take bovine cartilage, rinse it in PBS solution for 3 times, sterilize the cartilage with 75% ethanol aqueous solution for 30 minutes, rinse it in PBS solution for 3 times, and then place the cartilage in a -80°C refrigerator Freeze for 2 hours, thaw at 37°C for 0.5 hours, repeat the process of freezing and thawing 3 times;

[0050]2) Cut the obtained cartilage into thin slices, the thickness of which is not more than 2mm, soak the cartilage thin slices in 1mM potassium chloride solution for 3h;

[0051] 3) Soak the obtained cartilage slices in 0.01M sodium hydroxide aqueous solution containing 0.5MEDTA for 12h, the volume ratio of the cartilage slices to the solution is 1:50, rinse with PBS solution for 3 times; then place the cartilage slices in 0.5M HCl Soak the cartilage slices in 1M sodium chloride aqueous solution for 8 hours, the volume ...

Embodiment 2

[0057] The preparation method of injectable decellularized cartilage matrix microparticles comprises the following steps:

[0058] 1) Take pig cartilage, rinse it in PBS solution for 4 times, disinfect the cartilage with 0.5% peracetic acid aqueous solution for 30 minutes, rinse it in PBS solution for 3 times, and put the cartilage in a -80°C refrigerator Freeze in medium for 2h, thaw at 37°C for 0.8h, repeat freezing and thawing 3 times;

[0059] 2) Cut the obtained cartilage into thin slices, the thickness of which is not more than 2mm, soak the cartilage thin slices in 2mM potassium chloride solution for 4h;

[0060] 3) Soak the obtained cartilage slices in 0.01M sodium hydroxide solution containing 1MEDTA for 14h, the volume ratio of the cartilage slices to the solution is 1:60, rinse with PBS solution for 3 times; then place the cartilage slices in 0.6M HCl containing 1M Soak in sodium chloride solution for 8 hours, the volume ratio of cartilage slices to solution is 1:6...

Embodiment 3

[0066] The preparation method of injectable decellularized cartilage matrix microparticles comprises the following steps:

[0067] 1) Take bovine animal cartilage, rinse it in PBS solution for 3 times, disinfect the cartilage with 75% ethanol aqueous solution for 10 minutes, and then disinfect the cartilage with 0.2% peracetic acid aqueous solution for 20 minutes Minutes, rinsed in PBS solution for 3 times, then placed the cartilage in a -80°C refrigerator for 1.5h, thawed at 37°C for 0.5h, and repeated freezing and thawing 3 times;

[0068] 2) Cut the obtained cartilage into thin slices, the thickness of which is not more than 2mm, and soak the cartilage thin slices in 1.5 mM potassium chloride solution for 5 hours;

[0069] 3) Soak the obtained cartilage slices in 0.01M sodium hydroxide solution containing 1MEDTA for 12h, the volume ratio of the cartilage slices to the solution is 1:50, rinse with PBS solution for 3 times; then place the cartilage slices in 1M chlorine conta...

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Abstract

The invention discloses an injectable extracellular cartilage matrix particle and an application of the particle to implants. Cartilage is rinsed, sterilized, washed again, repeatedly frozen and thawed and cut into sheets, the sheets are soaked, decellularized, frozen, dried and grinded at low temperature to obtain the particle, and the particle can be further matched with implant auxiliary materials to serve as injectable extracellular cartilage implants. The matrix particle has good biocompatibility, structural components of the matrix particle are extracellular matrixes of cells, growth, proliferation and differentiation of the cells are facilitated, and the matrix particle has physiological effect on shape, phenotype and movement of the cells. The implants of the particle can serve as minimally invasive injection plastic implant materials, and the natural three-dimensional structure and natural active components of the cartilage are reserved, so that the implants have filling and tissue repairing functions.

Description

technical field [0001] The invention belongs to the field of regenerative medicine, in particular to injectable decellularized cartilage matrix microparticles and its application in implants. Background technique [0002] The application of injection microplastic surgery in cosmetic surgery has developed rapidly, and it has gradually shown its unique advantages of less trauma and faster recovery in the repair of soft tissue defects in the maxillofacial region, rhinoplasty and chin augmentation. Regardless of whether it is used alone or as a supplement to orthognathic and orthodontic repair operations, minimally invasive injection technology has broad application prospects in trauma reconstruction, repair of maxillofacial soft tissue defects, rhinoplasty and chin augmentation. [0003] Bovine collagen has been approved by the FDA as an injection filling material. Zyderm Ⅰ contains 3.5% bovine collagen and is suitable for superficial filling of periorbital and perioral wrinkl...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/36
CPCA61L27/3612A61L27/3687A61L27/50A61L2400/06
Inventor 杨伟红程飚王祥林陈大鹏邓小敏刘江辉
Owner 广州昕生医学材料有限公司
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