The invention relates to the technical field of
biology, and discloses a preparation method of
placenta hematopoietic stem cells. The method comprises the following steps: cleaning a pretreated
placenta by injection with an irrigating solution; injecting an enzymolysis solution from the
placenta artery to the placenta
vein until the enzymolysis solution flows out, tying the placenta
artery and
vein, and standing; loosening the placenta
artery and
vein, injecting the irrigating solution from the artery, collecting all the liquid at the vein, centrifugating, taking the
cell precipitate, recovering and purifying single karyocytes comprising hematopoietic stem cells sequentially by a
hydroxyethyl starch process and an erythrocyte
cracking process, and resuspending to obtain the placenta hematopoietic stem cells. The method improves the enzymolysis injection technique, selects multiple mutually-suitable components to constitute the novel enzymolysis solution which is used in the placenta
hematopoietic stem cell preparation process, can mobilize the hematopoietic stem cells inside the placenta to quickly enter the
blood circulation system within a short time, and enhances the single karyocyte quantity comprising hematopoietic stem cells, the
living cell quantity and the CD34 positive
cell quantity.