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69 results about "IRRIGATING SOLUTIONS" patented technology

Sterile irrigating solutions are sterile or nonpyrogenic isotonic solutions, made under sterile conditions. They are generally used for washing, irrigation and rinsing purposes.

Cleaning and devitalization of cartilage

InactiveUS20080077251A1Improve recellularizationBone implantDead animal preservationCellular DebrisMedicine
The invention is further directed to producing a cleaned, disinfected, and devitalized cartilage graft by optionally cleaning and disinfecting the cartilage graft; treating the cartilage graft in a pretreatment solution; treating the cartilage graft in an extracting solution; washing the extracted cartilage graft with a rinsing solution; and subsequently soaking the devitalized cartilage graft in a storage solution. The devitalized cartilage graft is essentially free from metabolically viable and/or reproductively viable cells and the rinsing solution is hypotonic solution or isotonic solution. The present invention is further directed to a cleaned, disinfected, and devitalized cartilage graft and a process for cleaning, disinfecting, and devitalizing cartilage grafts. The invention also relates to a process for repairing a cartilage defect and implantation of a cartilage graft into a human or animal by crafting the cartilage matrix into individual grafts, disinfecting and cleaning the cartilage graft, applying a pretreatment solution to the cartilage graft, removing cellular debris using an extracting solution to produce a devitalized cartilage graft, implanting the cartilage graft into the cartilage defect with or without an insertion device, and sealing the implanted cartilage graft with recipient tissue. The devitalized cartilage graft is optionally recellularized in vitro, in vivo, or in situ with viable cells to render the tissue vital before or after the implantation. The devitalized cartilage graft is also optionally stored between the removing cellular debris and the recellularizing steps.
Owner:LIFENET HEALTH

Irrigating solution, enzymatic hydrolysate and method for isolating placenta hematopoietic stem cells

The invention discloses an irrigating solution, an enzymatic hydrolysate and a method for isolating placenta hematopoietic stem cells. The irrigating solution consists of a penicillin-streptomycin double-antibody, phentolamine, vitamin C and PBS. The enzymatic hydrolysate consists of a basal culture medium, collagenase VIII, pancreatin, 1*heparin sodium, phentolamine, vitamin C and Z-VAD-FMK. The method comprises the following steps: irrigating and cleaning a preprocessed placenta with the irrigating solution; irrigating the enzymatic hydrolysate from placental artery until the enzymatic hydrolysate flows out of placental vein, ligating placental artery and vein and standing by; loosening the placental artery and vein and irrigating the irrigating solution from the artery, collecting all liquid from the vein, centrifuging and reserving cell sediments, and recovering and purifying mononuclear cells containing the hematopoietic stem cells sequentially by virtue of a hydroxyethyl starch method and a red blood cell lysis method; re-suspending and obtaining the placenta hematopoietic stem cells. The irrigating solution, the enzymatic hydrolysate and the method disclosed by the invention can significantly improve separation and extraction efficiencies, and a great amount of cells are obtained; the hematopoietic stem cells are high in content, high in survival rate and quite low in cell pollution rate.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD

Preparation method of placenta hematopoietic stem cells

The invention relates to the technical field of biology, and discloses a preparation method of placenta hematopoietic stem cells. The method comprises the following steps: cleaning a pretreated placenta by injection with an irrigating solution; injecting an enzymolysis solution from the placenta artery to the placenta vein until the enzymolysis solution flows out, tying the placenta artery and vein, and standing; loosening the placenta artery and vein, injecting the irrigating solution from the artery, collecting all the liquid at the vein, centrifugating, taking the cell precipitate, recovering and purifying single karyocytes comprising hematopoietic stem cells sequentially by a hydroxyethyl starch process and an erythrocyte cracking process, and resuspending to obtain the placenta hematopoietic stem cells. The method improves the enzymolysis injection technique, selects multiple mutually-suitable components to constitute the novel enzymolysis solution which is used in the placenta hematopoietic stem cell preparation process, can mobilize the hematopoietic stem cells inside the placenta to quickly enter the blood circulation system within a short time, and enhances the single karyocyte quantity comprising hematopoietic stem cells, the living cell quantity and the CD34 positive cell quantity.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD

Preparation method for placenta hemopoietic stem cell

The invention relates to the biotechnical field and discloses a preparation method for a placenta hemopoietic stem cell. The preparation method comprises the following steps: irrigating and cleaning a pre-processed placenta with an irrigating solution; irrigating a mobilizing agent from placental arteries until the mobilizing agent stops flowing from the placental veins and the placental arteries and veins are ligatured and stood; loosening the placental arteries and veins and grouting the same amount of mobilizing agent from the arteries, collecting all liquids from the veins, and centrifuging to get cell sediments; then, recycling and purifying single karyocyte comprising the hemopoietic stem cell through a hydroxyethyl starch process and a red blood cell lysis process in sequence, and re-suspending to obtain the placenta hemopoietic stem cell. According to the preparation method, the mobilizing agent irrigating process is improved, a plurality of mutually suitable components are selected to form a novel mobilizing agent for being applied to a preparation process of the placenta hemopoietic stem cell, the placenta hemopoietic stem cell inside the placenta can be mobilized and can quickly enter a blood circulating system, and the quantity of single karyocyte comprising the hemopoietic stem cell as well as quantity of living cells of the single karyocyte and quantity of CD34 positive cells is increased.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD

Sterile bladder irrigator

The invention provides a sterile bladder irrigator, which comprises a medical three-way valve, a urethral catheter, a liquid conveying tube and a drainage tube, wherein the urethral catheter, the liquid conveying tube and the drainage tube are connected with three connectors of the three-way valve; one connector of the three-way valve is an outer conical joint; the other two connectors of the three-way valve are inner conical connectors; the urethral catheter, the liquid conveying tube and the drainage tube are connected with the three connectors of the three-way valve through being tightly screwed via screw caps; the liquid conveying tube is used for feeding a flushing solution into the three-way valve from a liquid conveying bottle; the urethral catheter is used for feeding the flushing solution from the three-way valve into the bladder of a human body or guiding liquid in the bladder out of the human body; and the drainage tube is used for discharging out the solution from the urethral catheter. The urethral catheter, the liquid conveying tube and the drainage tube are connected with three connectors of the three-way valve through being tightly screwed via the screw caps, so that good sealing performance is guaranteed; in the whole operation process, the solution is not in contact with outside; the sterile operation is realized; the cleanness of the flushing solution is guaranteed; no fungal infection risk exists; and the use is safe and reliable.
Owner:JILIN UNIV

Irrigating solution, enzymatic hydrolysate and method for isolating placenta hematopoietic stem cells

ActiveCN105368781AIncrease the number of withdrawalsGuaranteed survival rateBlood/immune system cellsPenicillinHydrolysate
The invention discloses an irrigating solution, an enzymatic hydrolysate and a method for isolating placenta hematopoietic stem cells. The irrigating solution consists of a penicillin-streptomycin double-antibody, phentolamine, vitamin C and PBS. The enzymatic hydrolysate consists of a basal culture medium, collagenase I, collagenase II, heparin sodium, phentolamine, vitamin C and Z-VAD-FMK. The method comprises the following steps: cleaning blood inside a placenta with the irrigating solution; irrigating the enzymatic hydrolysate into the placenta and enzymatically hydrolyzing at room temperature; collecting the enzymatic hydrolysate, centrifuging and reserving cell sediments; re-suspending the cell sediments by virtue of the PBS, adding a hydroxyethyl starch solution, standing by, reserving and centrifuging upper liquid and discarding supernatant; re-suspending the cell sediments by virtue of a red blood cell lysis solution, promoting lysis to residual red cells, centrifuging and discarding supernatant; and re-suspending the cell sediments by virtue of a culture medium, centrifuging and discarding supernatant, so that the placenta hematopoietic stem cells are obtained. The irrigating solution, the enzymatic hydrolysate and the method disclosed by the invention can significantly improve separation and extraction efficiencies, and a great amount of cells are obtained; the hematopoietic stem cells are high in content, high in survival rate and quite low in cell pollution rate.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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