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Preparation method of mixed hydrogel biological material and application thereof

A technology of biological materials and mixed water, applied in the field of tympanic membrane repair biological materials and transplantation, can solve the problems of lack of mechanical strength and mechanical controllability, unsatisfactory effect of biological scaffolds, lack of biological activity, etc., and achieves strong mechanical properties and experimental methods. Easy to operate, good biocompatibility

Pending Publication Date: 2019-10-08
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic hydrogel materials have good mechanical properties, but they lack biological activity
Although the biological properties of biomaterial hydrogels can meet the requirements of cells, they lack mechanical strength and mechanical controllability.
Therefore, the effect of using a single material to prepare biological scaffolds is not ideal, and composite scaffolds have gradually become a research hotspot.

Method used

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  • Preparation method of mixed hydrogel biological material and application thereof
  • Preparation method of mixed hydrogel biological material and application thereof
  • Preparation method of mixed hydrogel biological material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Weigh 1% w / v HAMA solid, add it to a 5ml centrifuge tube containing 1ml deionized water, dissolve in a 40°C water bath, shake and mix;

[0035] (2) Weigh 15% w / v GelMA solid, add it to the dissolved solution of step (1), dissolve in a 40°C water bath, shake and mix;

[0036] (3) Weigh 12% w / v decellularized cartilage matrix powder with a particle size of less than 40 μm, add it to the dissolved solution in step (2), shake and mix;

[0037] (4) Add 0.1% w / v photocrosslinker Ig2959 to the solution in step (3), shake and mix;

[0038] (5) Soak the climbing sheet with a diameter of 14mm in 3% w / v PVA aqueous solution, take it out and dry it in an oven at 70°C;

[0039] (6) Drop 45 μl of the material prepared in step (4) onto the slide prepared in step (5), and quickly cover with another slide;

[0040] (7) Place the material prepared in step (6) under a 365nm ultraviolet lamp and irradiate it for 2 minutes, and the photocrosslinking is completed;

[0041] (8) The mat...

Embodiment 2

[0043] (1) Weigh 1% w / v HAMA solid, add it to a 5ml centrifuge tube containing 1ml deionized water, dissolve in a 40°C water bath, shake and mix;

[0044] (2) Weigh 5% w / v GelMA solid, add it to the dissolved solution of step (1), dissolve in a 40°C water bath, shake and mix;

[0045] (3) Weigh 3% w / v decellularized cartilage matrix powder with a particle size of less than 40 μm, add it to the dissolved solution in step (2), shake and mix;

[0046] (4) Add 0.1% w / v photocrosslinker Ig2959 to the solution in step (3), shake and mix;

[0047] (5) Soak the climbing sheet with a diameter of 14mm in 3% w / v PVA aqueous solution, take it out and dry it in an oven at 70°C;

[0048] (6) Drop 45 μl of the material prepared in step (4) onto the slide prepared in step (5), and quickly cover with another slide;

[0049] (7) Place the material prepared in step (6) under a 365nm ultraviolet lamp and irradiate it for 2 minutes, and the photocrosslinking is completed;

[0050](8) The materi...

Embodiment 3

[0052] (1) Weigh 2% w / v HAMA solid, add it to a 5ml centrifuge tube containing 1ml deionized water, dissolve in a 45°C water bath, shake and mix;

[0053] (2) Weigh 9% w / v GelMA solid, add to the dissolved solution of step (1), dissolve in 45°C water bath, shake and mix;

[0054] (3) Weigh 10% w / v decellularized cartilage matrix powder with a particle size less than 40 μm, add it to the dissolved solution in step (2), shake and mix;

[0055] (4) Add 0.1% w / v photocrosslinker Ig2959 to the solution in step (3), shake and mix;

[0056] (5) Soak the climbing sheet with a diameter of 14mm in 3% w / v PVA aqueous solution, take it out and dry it in an oven at 70°C;

[0057] (6) Drop 45 μl of the material prepared in step (4) onto the slide prepared in step (5), and quickly cover with another slide;

[0058] (7) Place the material prepared in step (6) under a 365nm ultraviolet lamp and irradiate it for 2 minutes, and the photocrosslinking is completed;

[0059] (8) The material obt...

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Abstract

The invention provides a preparation method of a mixed hydrogel biological material and an application thereof. The material is prepared by mixing an acellular cartilage matrix, a Gelatin Methacryloyl(GelMA) and Hyaluronic Acid Methacryloyl (HAMA) in a certain ratio. The preparation method of all materials of the invention has the characteristics of safety, non toxicity, easy operation and low immunogenicity, can be applied to repair of clinical tympanic membrane perforation, replaces traditional treatment methods, and has wide application prospects.

Description

technical field [0001] The invention relates to the field of tympanic membrane repair biological material and transplantation, in particular to a preparation method and application of a mixed hydrogel biological material. Background technique [0002] In recent years, there have been more and more tympanic membrane perforations caused by trauma, middle ear inflammation and iatrogenic injuries. There are many ways to treat tympanic membrane perforation, which can be roughly divided into non-surgical patches (cotton pads, silk, gelatin sponge, seabuckthorn oil, Gauze, garlic film, egg film, artificial skin, hydrocolloid dressing, fibrin glue, etc.) and surgical repair (autologous materials include temporalis muscle fascia, tragus cartilage-perichondrium, skin, adipose tissue, etc., allogeneic materials are the same Allogeneic materials and biosynthetic materials, etc.) two methods. Until now, tympanic membrane repair using autologous tissue is the common method for repairing ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/20A61L27/22A61L27/50A61L27/52
CPCA61L27/3612A61L27/3641A61L27/20A61L27/222A61L27/50A61L27/52A61L2430/14C08L5/08
Inventor 方练李花琼余媛祝梦渝吴存造
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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