Method for breeding durable resistance material for wheat stripe rust

A wheat stripe rust and resistance technology, which is applied in the fields of botany equipment and methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem that the resistance cannot reach the resistance level of wheat varieties, and achieve excellent The effect of intermediate materials

Active Publication Date: 2017-08-18
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the problem is that the resistance provided by a single or a small number of adult plant res...

Method used

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  • Method for breeding durable resistance material for wheat stripe rust
  • Method for breeding durable resistance material for wheat stripe rust

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. High-pressure identification of mixed races at room temperature and selection of resistant material P in the whole growth period 1 test.

[0029] Mix the collected strains of CYR29, CYR30, CYR31, CYR32, CYR33, V26, and Shuiyuan 14 for later use, plant the materials to be screened in potted plants in the greenhouse, sow 5-8 seeds for each material, and wait to grow to 1 core 1 In the leaf stage, use the smear method to inoculate the mixed strains, humidify with a humidifier for 24 hours after inoculation, keep dark conditions, control the room temperature at 10-16°C, then adjust the room temperature to about 20°C, and light for 12 hours. After the susceptible control Chuanyu 12 fully developed the disease, the seedling stage disease resistance of the materials to be tested was identified, and excellent materials with good seedling stage disease resistance such as 10P4-8, 13P2-7, and 14P532 were screened out.

Embodiment 2

[0030] Embodiment two, P 1 ×P 2 Cross to obtain F1 generation test.

[0031] Taking the excellent materials (10P4-8, 13P2-7, 14P532, etc.) screened out in Example 1 as the parent P 1 , with known Yr18-containing adult plant and resistant materials (such as 13KG458, 13KG662, etc.) as the male parent P 2 , carry out hybrid combination configuration to obtain F1 material.

Embodiment 3

[0032] Embodiment three, backcross test and field identification

[0033] Utilize the F1 material obtained in embodiment two as female parent, with the same adult plant stage and resistant type material (such as 13KG458, 13KG662 etc.) containing Yr18 described in embodiment two P 2 As the male parent, cross to obtain BC 1 f 1 Germplasm; the next generation with BC 1 f 1 as the female parent, with P 2 As the male parent, cross to obtain BC 2 f 1 ; and so on until BC 6 f 1 , to obtain the material YrP with stable target traits of disease resistance and agronomic traits. The above implementation process was carried out under the induction conditions of dominant multi-race species in the field, and the selected backcross female parent had high stripe rust resistance phenotypes at the seedling stage and after heading. The induced material and the backcross test material were planted alternately, and the mixed dominant race was inoculated by smearing method at the 1-heart-1...

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Abstract

The invention belongs to the field of wheat disease resistance breeding, and in particular discloses a method for effectively breeding a durable resistance breeding material for stripe rust, wherein the material is obtained by combination of a seedling stage disease-resistant gene and adult-plant stage disease-resistant genes such as Yr18 by using high-pressure identification and multi-generation backcrossing techniques of hybrid microspecies. The method can provide an excellent intermediate material for rust-resistant breeding.

Description

technical field [0001] The invention belongs to the field of wheat disease-resistant breeding, and in particular relates to a method for breeding wheat stripe rust durable resistant materials. Background technique [0002] Wheat stripe rust is a fungal airborne disease caused by Puccinia striiformis f.sp.tritici, which can lead to regional wheat yield reduction of 0.1-5%, and in severe cases, yield reduction of 5-25%. my country is the largest wheat stripe rust endemic area in the world, and there have been many outbreaks of stripe rust pandemics. According to statistics, the average occurrence area of ​​stripe rust in my country from 2002 to 2012 was about 4.2 million hm 2 / year, posing a huge threat to wheat production. The characteristic that urediospores of wheat stripe rust can be spread over long distances with air currents makes it possible for the disease to quickly cause the disease to circulate in the region, and it is a large-scale epidemic disease. [0003] Bre...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H1/02
CPCA01H1/02C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 蒲宗君郑建敏罗江陶杨恩年李式昭
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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