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RAPD marker research of wheat powdery mildew resistant gene Pm23

A powdery mildew resistance gene and disease resistance gene technology, applied in the field of RAPD marker research, can solve problems such as limited application

Inactive Publication Date: 2010-06-30
李祥
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The gene has been located on chromosome 5A, but so far, no molecular marker linked to it has been found, which limits its application in disease resistance breeding to a certain extent

Method used

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  • RAPD marker research of wheat powdery mildew resistant gene Pm23
  • RAPD marker research of wheat powdery mildew resistant gene Pm23
  • RAPD marker research of wheat powdery mildew resistant gene Pm23

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Experimental program
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Embodiment Construction

[0099] 1. Materials and methods

[0100] 1.1 Experimental materials

[0101] The wheat line Pm23 with the disease-resistant gene Pm23 was used as the disease-resistant parent, and the wheat variety Chancellor without any disease-resistant gene was used as the susceptible parent (also used as a susceptible control) to prepare a hybrid combination Pm23×Chancellor, F 1 Generation selfing, obtained 101 strains of F 2 Individual plants are used as segregated populations for screening of molecular markers and genetic linkage analysis.

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Abstract

Pm23 is a wheat powdery mildew resistant gene tested in an ordinary wheat strain in China. The gene represents high resistance or immune on the powdery mildew prevalent in a plurality of wheat areas in the world, located to 5A chromosome. In the research, the wheat strain Pm23 with the disease resistant gene Pm23 is used as a disease resistant parent and the wheat breed Chancellor without any disease resistant gene as a susceptible parent for confecting hybridized combination, and RAPD analysis is carried out on the resistance gene Pm23 by a bulked segregant analysis (BSA) method. The augmentation result of 350 ten-base random primers in an induction resistant pool shows that coupling phase marker OPE051100 may be tightly linked with Pm23. RAPD analysis on F2 segregation population shows that the linkage distance between the marker and the Pm23 gene is 10.65+3.25cM. The marker can be effectively used in the molecular marker-assisted selection of wheat disease resistant breeding.

Description

technical field [0001] The RAPD marker research of the wheat powdery mildew resistance gene Pm23 of the present invention uses cluster separation analysis (BSA) to carry out RAPD analysis on the resistance gene Pm23, in order to find the RAPD marker closely linked with Pm23, which is applied to the practice of molecular marker-assisted selection breeding, and belongs to The field of crop genetics and breeding. Background technique [0002] Wheat powdery mildew resistance gene Pm23 was found in common wheat cultivars. This gene is immune to the dominant race of wheat powdery mildew that is currently prevalent in major wheat regions in my country, and because the carrier variety is common cultivated wheat, there is no linkage of adverse genes Therefore, Pm23 is a rare high-quality resistance source in wheat powdery mildew resistance breeding. The gene has been located on chromosome 5A, but so far, no molecular marker linked to it has been found, which limits its application in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李祥
Owner 李祥
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