Replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus and application
A technology of gene fragments and leaf viruses, which is applied in the field of efficiently stimulating the replicase gene fragments and applications of wheat resistance to yellow mosaic virus disease, and can solve the problems of resistance loss of disease-resistant varieties and other issues
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[0008] Below in conjunction with accompanying drawing and embodiment the present invention will be further described:
[0009] The invention provides an antisense wheat yellow mosaic virus replicase gene fragment, which can cause sustained and high-efficiency resistance to wheat yellow mosaic virus disease after being expressed in wheat cells. The invention includes the nucleotide sequence of the antisense wheat yellow mosaic virus replicase gene, the homology analysis of the nucleotide chain and the DNA cloning and expression vector construction of the antisense RNA sequence. Among them, the source of the virus used is wheat leaves infected with wheat yellow mosaic virus in Lixiahe Research Institute, Yangzhou City, Jiangsu Province. After extracting the total RNA of the infected leaves, the cDNA fragment was obtained by amplifying the specific sequence on the corresponding positive-sense RNA strand of this fragment. The part of the replicase gene on the positive sense RNA ch...
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