Replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus and application

A technology of gene fragments and leaf viruses, which is applied in the field of efficiently stimulating the replicase gene fragments and applications of wheat resistance to yellow mosaic virus disease, and can solve the problems of resistance loss of disease-resistant varieties and other issues

Inactive Publication Date: 2012-07-25
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +2
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  • Application Information

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Problems solved by technology

The third is the continuous emergence of new viruses and pathogenic strains leading to the loss of resistance of resistant varieties

Method used

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  • Replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus and application
  • Replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus and application
  • Replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus and application

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Embodiment Construction

[0008] Below in conjunction with accompanying drawing and embodiment the present invention will be further described:

[0009] The invention provides an antisense wheat yellow mosaic virus replicase gene fragment, which can cause sustained and high-efficiency resistance to wheat yellow mosaic virus disease after being expressed in wheat cells. The invention includes the nucleotide sequence of the antisense wheat yellow mosaic virus replicase gene, the homology analysis of the nucleotide chain and the DNA cloning and expression vector construction of the antisense RNA sequence. Among them, the source of the virus used is wheat leaves infected with wheat yellow mosaic virus in Lixiahe Research Institute, Yangzhou City, Jiangsu Province. After extracting the total RNA of the infected leaves, the cDNA fragment was obtained by amplifying the specific sequence on the corresponding positive-sense RNA strand of this fragment. The part of the replicase gene on the positive sense RNA ch...

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Abstract

The invention relates to a replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus and application. The replicase gene segment is a nucleotide sequence of the replicase gene segment, which can excite the wheat yellow to resist the mosaic virus. The replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus has the nucleotide sequence disclosed by SEQ ID NO:1. According to the replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus, the nucleotide sequence is connected to the downstream of an eukaryotic expression promoter to carry out that a common foreign gene expresses in a wheat body, and a wheat plant for expressing the sequence segment is applied on the aspect of effectively resisting the virus invasion. The replicase gene segment for efficiently exciting wheat yellow to resist mosaic virus has the beneficial effect that the nucleotide sequence from a wheat mosaic virus conserved sequence is used for exciting the disease resistance of the wheat. Because the virus copies the required replicase gene segment, the replicase gene segment has high conservatism and stability. The wheat disease resistance excited by the segment has broad spectrum disease resistance and long-term stability.

Description

technical field [0001] The invention relates to the field of biotechnology, and mainly relates to a replicase gene fragment for efficiently stimulating wheat resistance to yellow mosaic virus disease and its application. Background technique [0002] Wheat is the world's largest food crop. Like other crops, wheat is often infested by diseases and insect pests, resulting in reduced yield and quality. Among them, soil-borne mosaic virus diseases transmitted by Polymyxa graminearum are the most widely distributed, most diverse and most harmful. In my country, soil-borne mosaic virus disease has been one of the important diseases frequently occurring in winter wheat planting areas such as Henan, Jiangsu, Anhui, Shandong, etc. since it was discovered in the 1970s, and has become an important constraint affecting the high yield and quality of wheat in our country. one of the factors. It is estimated that the annual incidence of soil-borne viral diseases exceeds 30 million mu, re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/82A01H5/00C12R1/94
Inventor 陈剑平陈炯徐惠君程顺和马有志孙丽英吴宏亚陈明张晓祥张伯桥
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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