Wheat disease-resistant gene Lr-L1 and its use

Abstract

The present invention relates to plant gene engineering technology, and is especially one kind of wheat disease resisting gene Lr-L1 and its application. The wheat disease resisting gene Lr-L1 has cDNA of whole length 2326 bp, including 5'-noncoding area of 135 bp, 3'-noncoding area of 249 bp, coding area of 1911 bp, terminator, and polyadenyl acid tail of 28 bp. The 3'-noncoding area includes includes 3 typical tailing signal sequences and can reinforce the tailing process after gene transcription. The wheat disease resisting gene Lr-L1 may be inserted into the downstream of different types of promoter to constitute plant expression vector for plant disease resisting improvement in gene engineering technology, and may be cloned for making wheat gene chip for wheat disease resistance and other research.

Description

[0001] One. Technical field: the present invention relates to the field of plant genetic engineering technology, in particular to a kind of wheat disease resistance gene Lr-L1 and its application. 2. Background technology:

[0002] Plant transgenic research originated in the early 1980s. In 1983, Zambryski obtained the first transgenic plant in the world. In 1985, Horch created the leaf disc method in Agrobacterium transformation. Since then, the Agrobacterium-mediated method, virus Mediated method, PEG-mediated method, electroporation method, microinjection method, pollen tube channeling method, ultrasonic method, gene gun method, etc., the species successfully transgenic are constantly expanding, involving more than 50 species and more than 110 kinds of plants, of which , the transgenic plants obtained by the Agrobacterium-mediated method accounted for more than 85% of the total number of transgenic plants, and the transgenic plants obtained by the biolistic method accounted ...

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