Method for integrating focusing and detection of cells and miniaturized system thereof

Abstract

The invention discloses a method for integrating focusing and detection of cells and a miniaturized system thereof. The miniaturized system comprises a microfluidic chip, a data acquisition card, a miniature computer and a sample injection device, wherein the microfluidic chip is formed by a flow channel layer, a substrate layer and a PCB (Printed Circuit Board) which are aligned and packaged in sequence; the flow channel layer is provided with an asymmetric sinusoidal flow channel, a detection main flow channel, polyelectrolyte gel and a conductive-fluid storage pool; the polyelectrolyte gel, the conductive-fluid storage pool and a silver-silver chloride wire form a detection electrode; the silver-silver chloride wire is connected with the data acquisition card and the miniature computer by a transimpedance amplifier and a differential amplifier so as to form a differential impedance detection circuit of the cells; the miniature computer is used for realizing generation of pseudorandom excitation signals, processing of system response signals and analysis and display of multi-performance parameters of the cells. The method and the miniaturized system disclosed by the invention integrate the functions of focusing and detection of the cells, realizes miniaturization and portability of the system and can be widely used for biological study of blood cells and rare cells.

Description

technical field [0001] The invention relates to a miniaturized cell detection system integrating asymmetric sinusoidal flow channel inertial focusing and pseudo-random sequence electrical impedance measurement technology, which belongs to the field of microfluidic chip, biological particle control and electrical impedance detection. Background technique [0002] The analysis of biochemical and biophysical characteristics at the single-cell level can effectively clarify the differences between cells and reveal the function and state of cells, which is of great significance for the study of cell physiology and pathology. Microfluidic chips have become an important technical platform for single-cell research because of their micron-scale channels that match the cell scale. So far, researchers have successfully applied biochemical analysis methods such as immunocytochemistry, reverse transcription polymerase chain reaction, and fluorescence in situ hybridization to microfluidic ...

AI Technical Summary

Problems solved by technology

Although this method can obtain accurate cell electrical characteristics, it takes a long time to detect and cannot characterize the real-time state of cells

Dynamic single-frequency measurement can achieve high-throughput detection of cell flow dynamics, but due to the limited frequency of measurement, it is impossible to obtain a complete cell impedance spectrum

In addition, the current microfluidic cell electrical impedance detection system generally requires the use of commercially expensive instruments such as lock-in amplifiers, spectrum analyzers, etc., resulting in a huge detection system, which makes it difficult to achieve instant clinical diagnosis

Images