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Multifunctional micro-fluidic chip for cell migration and invasion assay

A microfluidic chip and cell migration technology, applied in laboratory containers, tissue cell/virus culture devices, laboratory utensils, etc., can solve cell or extracellular matrix leakage experiments, cell or extracellular matrix Leakage, failure and other problems, to achieve the effect of low production cost, multi-functional experiment success rate, and reliable operation

Active Publication Date: 2014-03-12
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, sudden changes in the temperature difference during the experimental operation, for example, during the culture of the chip from room temperature to 37 °C, the cell suspension or uncured extracellular matrix (ECM) and the surface tension of the microdam will change significantly, which will easily cause damage to cells or cells. Extracellular Matrix (ECM) Leakage Affects Experimental Results
At the same time, when the chip is manually injected with a pipette or a syringe, it is easy to cause pressure fluctuations in the chip channel, causing cells or extracellular matrix (ECM) to leak out and the experiment fails, which poses a great challenge to the operator's technical proficiency

Method used

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  • Multifunctional micro-fluidic chip for cell migration and invasion assay
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  • Multifunctional micro-fluidic chip for cell migration and invasion assay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Cell migration experiment: The microfluidic chip used is designed and prepared by our laboratory. The chip is composed of three layers of irreversible bonding, the upper layer is a polydimethylsiloxane polymer material (PDMS) with a microvalve structure, and the middle layer is a polydimethylsiloxane with a fluid channel structure with a thickness of 80~500μm Based on siloxane polymer film, the lower layer material is ordinary glass, quartz glass or optical glass. Use a pipette to pass mouse tail collagen I with a concentration of 1 mg / ml into the first cell culture channel 6, the second cell culture channel 7, and the third cell culture channel 8, and place it in an ultra-clean bench to dry overnight to make the collagen The protein coats the cell culture area. On the second day, close the first microvalve 4 and the second microvalve 5 of the chip, and then infuse the HepG2 suspension of liver tumor cells with a certain density into the first cell culture channel 6 and...

Embodiment 2

[0034] Cell invasion experiment: HepG2 cells were digested and enriched by centrifugation to the required concentration, and then placed in an ice bath for later use. The rat tail collagen I in the ice bath was configured to a concentration of 4mg / ml, then 1:1 The ratio of (V:V) to the cell suspension is best to prepare a cell-collagen solution with a concentration of 2mg / ml, and mix well. Close the first microvalve 4 and the second microvalve 5 of the chip, then pass the solution into the second cell culture channel 7, put it in the 37℃ cell culture incubator, wait for the gel to solidify after 20 minutes, and then open the first microvalve 4. The second microvalve 5, through the first cell culture channel 6 and the third cell culture channel 8 at a flow rate of 0.1μl / min into the complete cell culture solution, 4 days later, the first cell culture channel 6 into the drug-containing Serum-free culture medium, the third cell culture channel 8 still enters the complete cell cult...

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Abstract

The invention discloses a multifunctional micro-fluidic chip for cell migration and an invasion assay. The multifunctional micro-fluidic chip comprises a first polydimethylsiloxane (PDMS) film layer, a second PDMS film layer and a glass substrate layer, wherein the first PDMS film layer, the second PDMS film layer and the glass substrate layer are sequentially and irreversibly bonded to form an integral structure; a first micro valve and a second micro valve are arranged on the first PDMS film layer; a first cell culture channel, a second cell culture channel and a third cell culture channel are formed in the second PDMS film layer; the first micro valve is positioned on the upper part of the joint of the first cell culture channel and the second cell culture channel; the second micro valve is positioned on the upper part of the joint of the second cell culture channel and the third cell culture channel. The multifunctional micro-fluidic chip has the advantages of being flexible and easy to operate, reliable to operate, low in manufacturing cost, high in assay success rate, multiple in functions and the like, has high biological study and economic values and is expected to provide a novel research platform for development of medicines for inhibiting tumor cell invasion in the future.

Description

Technical field [0001] The invention relates to the technical field of medical equipment, in particular to a multifunctional microfluidic chip for cell migration and invasion experiments. Background technique [0002] The key to tumor metastasis is the ability of tumor cell migration and invasion. Tumor metastasis seriously affects the survival rate of patients. Clinical treatment has always been the focus of research on how to inhibit tumor cell migration and metastasis. There are several commonly used models for studying cell migration and invasion in vitro, such as scratch experiment and transwell chamber transmembrane detection, which are widely used to study cell migration and invasion. The cell scratch method is a conventional method for studying cell migration. When the cells converge to form a monolayer on the culture medium, use a pipette tip to make a scar on the cell, and then observe the cell migration. However, this method will cause mechanical damage to the cells a...

Claims

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Application Information

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IPC IPC(8): C12M3/00
CPCB01L3/502738B01L2200/0647B01L2300/0887B01L2400/06G01N33/5029G01N33/5032
Inventor 孟宪生马立东王乙同包永睿王帅
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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