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Terahertz metamaterial device for detecting circulating tumor cells and preparation method thereof

A tumor cell and metamaterial technology, applied in the field of terahertz metamaterial devices for detecting circulating tumor cells and their preparation, can solve the problems of cumbersome, time-consuming, manual labor, etc., and achieve the effect of high sensing sensitivity and remarkable results

Pending Publication Date: 2018-10-09
TAISHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that this process is divided into multiple steps, which is relatively cumbersome.
[0004] At present, the characterization of the isolated circulating tumor cells is still mainly based on microscope observation, which relies on manual work and takes a lot of time
The isolated circulating tumor cells are aggregated together, and only their population characteristics can be analyzed, and it is difficult to analyze the individual behavior characteristics of individual cancer cells after they are separated from the cell tissue
[0005] At present, most terahertz metamaterial devices with planar structure are used as sensors for sensing biological samples. On the one hand, it is not conducive to using the structural characteristics of the device itself to capture the sensing object, and it is also not conducive to confining the sensing object to the sensitive position of the device (such as the opening). The opening of the resonant ring), for micron-scale spherical sensors, such as biological cells, the resonant ring structure in the planar structure can only sense the bottom of the sphere, and these problems will lead to a decrease in sensing sensitivity; on the other hand, the planar terahertz The metamaterial structure also does not have the ability to filter and separate large and small cells (particles), and cannot capture sensors from complex environments, so it is used as a sensor with a low degree of automation

Method used

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  • Terahertz metamaterial device for detecting circulating tumor cells and preparation method thereof
  • Terahertz metamaterial device for detecting circulating tumor cells and preparation method thereof
  • Terahertz metamaterial device for detecting circulating tumor cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 and Embodiment 2

[0064] The unit structure in the capture area 1-2 is a pair of split resonant rings (such as Figure 4 , 5 , 7, 8).

[0065] The dimensions of each split resonant ring are shown in Table 1.

[0066] (The following dimensions 1. Ensure that the resonance frequency is in the terahertz range. 2. The opening range ensures that small red blood cells will not enter by mistake and cannot be filtered.)

[0067]

Line Width (microns)

Opening (micron)

Outer ring side length (diameter) (microns)

the smallest

2

2

20

maximum

20

8

80

[0068] The narrowest distance between the pair of split resonant rings should ensure that 8 μm red blood cells pass through and 20 μm cancer cells get stuck. So the narrowest point is 8~20μm.

[0069] The shortest distance to the surrounding adjacent split resonant ring pairs: 20~50μm. If it is too small, cancer cells cannot pass through it; if it is too large, there will be too few resonance struc...

Embodiment 2

[0074] Such as Figure 6 As shown, the inlet guide area 1-1 and the outlet guide area 1-3 of the film adopt a cylinder with a diameter of 70 μm as a spacer for dispersing liquid. Such as Figure 6 As shown (dotted line part), the line connecting the centers of three adjacent cylinders forms a regular triangle with a side length of 140 μm, and forms an array according to this rule. Such as Figure 7 As shown, the capture area 1-2 is composed of a pair of square ring-shaped cylinders with opposite openings as a unit structure, and the opening of each pair of square rings is used as a clamping position, according to Figure 7 The regularity shown forms the array composition. Such as Figure 8 As shown, the outer side length of the square ring is 30 μm, the inner side length is 24 μm, the inner side length at the opening is 20 μm, and the distance between the ring centers is 42 μm. Array Repeat Period ( Figure 7 Dashed box in ) is 102 μm x 60 μm. The cell structure is late...

Embodiment 3

[0077] The unit structure in the capture area 1-2 is a double split resonant ring ( Figure 9 ).

[0078] The diameter of the outer ring of the unit structure is 70-90 μm, and the line width is 5-15 μm. The opening is 8-20 μm to retain larger cancer cells through smaller red blood cells, and the upper and lower openings do not require symmetry.

[0079] The distance between the unit structure and the adjacent unit structure: 20~50μm. If it is too small, cancer cells cannot pass through it; if it is too large, there will be too few resonance structures in the same area, and the spectral resonance intensity will be weak.

[0080] Such as Figure 9 As shown, the difference from Embodiment 1 is that the capture area 1-2 is composed of a single double-opened annular cylinder as a unit structure, and the opening of the ring facing the side of the inlet diversion area is the locking position. according to Figure 9 The patterns shown form an array. The outer diameter of the dou...

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Abstract

The invention provides a terahertz metamaterial device for detecting circulating tumor cells and a preparation method thereof. The device is mainly characterized with a stereo terahertz metamaterial,supplemented by a liquid diversion region. Only a small amount of blood sample is introduced from an inlet hole in a cover plate, and then blood cells of smaller size can be filtered according to structural features. Cancer cells of larger size are fixed at the sensitive positions, namely the clamping positions, of a metamaterial resonant structure. The cancer cells in the sample can be detected by comparing spectral changes before and after the sample is introduced. In addition, the device enables single separation of cancer cells, and can be used for subsequent biological study of individualcancer cells, so as to master the trait changes of each individual cancer cell stimulated by external conditions based on spectral analysis. Compared with the prior art, the device has high sensing sensitivity, and has remarkable effects when being used for fixed-point capture or large cell monitoring.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a terahertz metamaterial device for detecting circulating tumor cells and a preparation method thereof. Background technique [0002] Circulating tumor cells are a kind of rare cells that exist in the peripheral blood of tumor patients but are rare in number but have important clinical significance. It is a tumor cell that spreads into the peripheral blood circulation from tumor lesions, and can develop into tumor metastatic lesions under certain conditions. Circulating tumor cells in the blood suggest the existence of tumors and possible metastasis in the body, and more than 90% of cancer deaths in clinical practice are caused by metastasis; Therefore, capturing and detecting circulating tumor cells from blood has attracted more and more attention. [0003] At present, it is already a difficult problem to separate circulating tumor cells from blood. The Canpatrol CTC...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
CPCG01N21/255
Inventor 朱梅全保刚刘安成宿纪松
Owner TAISHAN UNIV
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