Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Prokaryotically produced antibodies and use thereof

a technology of prokaryotically produced antibodies and antibodies, which is applied in the field of molecular biology and protein technology, can solve the problems of poor yield of reconstituted tetrameric antibodies, i>e. coli /i>would not be a useful system for making intact antibodies, and both approaches have limitations

Inactive Publication Date: 2005-08-04
GENENTECH INC
View PDF17 Cites 33 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0010] The present invention addresses the need for producing intact antibodies in prokaryotic organisms. In one embodiment, the invention provides a process for producing an immunoglobulin in a prokaryotic host cell, comprising using a uniquely designed separate cistron expression vector. The separate cistron expression vector of the invention comprises a polynucleotide expression cassette, which comprises a first promoter-cistron pair for expression of an immunoglobulin light chain and a second promoter-cistron pair for expression of an immunoglobulin heavy chain, whereby expression of the light chain and heavy chain are independently regulated by separate promoters. Each cistron within the expression cassette polynucleotide comprises a translation initiation region (TIR) operably linked to the nucleic acid sequence coding for the light chain or heavy chain of the full length antibody. In some embodiments, the TIR sequences within the expression vector of the invention are manipulated so to provide different translational strength combinations for light and heavy chains. Many prokaryotic organisms are suitable as hosts for the expression vector of the invention. Preferably, the host is a gram-negative bacteria. More preferably, the host is E. coli. In one embodiment, the host cell is a genetically altered E. coli strain suitable for large quantity production of heterologous proteins. A number of promoters can be used for the expression vector of the invention. A preferred promoter is the E. coli PhoA promoter.

Problems solved by technology

Because of the complex feature and large size of an intact antibody, it is often difficult to achieve proper folding and assembly of the expressed light and heavy chain polypeptides, resulting in poor yield of reconstituted tetrameric antibody.
Furthermore, since antibodies made in prokaryotes are not glycosylated, thus lacking the effector functions, the art has suggested-that E. coli would not be a useful system for making intact antibodies.
However, both of these approaches have limitations.
Fc mutants may elicit undesirable immune response because of the residue changes in the primary sequence.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Prokaryotically produced antibodies and use thereof
  • Prokaryotically produced antibodies and use thereof
  • Prokaryotically produced antibodies and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

Construction of Expression Vectors

[0165] Various expression vectors were made for the expression of antibodies specific to tissue factor (anti-TF antibody) and antibodies specific to vascular endothelial cell growth factor (anti-VEGF antibody). For each vector construction, an expression cassette was cloned into the framework of the E. coli plasmid pBR322 at the EcoRI site. Sutcliffe (1978) Cold Spring Harbor Symp. Quant. Biol. 43:77-90. Each expression cassette contains at least the following components: (1) a phoA promoter for the control of transcription; (2) a Shine-Dalgarno sequence from the E. coli trp or the heat stable enterotoxin 11 (STII) gene, or a combination of both, for translation initiation; and (3) a λt0 terminator to end transcription. The basic components of bacterial expression cassettes are known in the art and have been described in, for example, Kikuchi et al., Nucleic Acids Res. 9(21):5671-5678 (1981) (for phoA promoter); Scholtissek and Grosse, Nucleic Acid...

example 2

E. coli Expression of Full Length Antibodies Using Polycistronic Vectors

[0170] Full length antibodies were first made in E. coli using polycistronic vectors derived from a published vector, pAK19, according to the methods described in Example 1. Small scale inductions were first performed to evaluate and compare the expression levels obtained with the various constructs.

Materials and Methods

[0171] For small scale expression of each construct, the E. coli strain 33D3 (W3110 ΔfhuA (ΔtonA) ptr3 lac Iq lacL8 ΔompTΔ(nmpc-fepE) degP41 kanR) was used as host cells. Following transformation, selected transformant picks were inoculated into 5 ml Luria-Bertani medium supplemented with carbenicillin (50 ug / ml) and grown at 30° C. on a culture wheel overnight. Each culture was then diluted (1:50 or 1:100) into C.R.A.P. phosphate-limiting media (3.57 g (NH4)2SO4, 0.71 g NaCltrate-2H2O, 1.07 g KCl, 5.36 g Yeast Extract (certified), 5.36 g HycaseSF-Sheffield, adjusted pH with KOH to 7.3, qs to...

example 3

E. coli Expression of Full length Antibodies Using Separate Cistron Vectors

[0181] To construct the separate cistron vectors with modulated TIR strength combinations, a preferred TIR strength for secretion of each individual chain was first determined in a series of single cistron plasmids constructed to express light or heavy chain only (FIG. 5). A series of single cistron plasmids with various TIRs was therefore constructed for the individual expression of both anti-TF light and heavy chains. Methods and materials used for vector construction and protein expression were similar to those used for polycistronic vector expressions, which has been described in Examples 1 and 2 above.

[0182] The range of TIR strengths tested extended from a relative strength of I to a maximum relative strength of 13. Reduced whole cell lysates from induced cultures transformed with these constructed plasmids were analyzed by SDS-PAGE and the results are shown in FIG. 6. For both heavy and light chain, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
temperatureaaaaaaaaaa
temperatureaaaaaaaaaa
temperatureaaaaaaaaaa
Login to View More

Abstract

The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.

Description

FIELD OF THE INVENTION [0001] The present invention relates generally to the fields of molecular biology and protein technology. More specifically, the invention concerns recombinantly produced antibodies and uses thereof. BACKGROUND OF THE INVENTION [0002] Recent years have seen increasing promises of using antibodies as diagnostic and therapeutic agents for various disorders and diseases. Many research and clinical applications require large quantities of functional antibodies, thus calling for large scale, economic production systems to be employed. Particularly useful is the recombinant production of antibodies using a variety of expression hosts, ranging from prokaryotes such as E. coli or B. subtilis, to yeast, plants, insect cells and mammalian cells. Kipriyanov and Little (1999) Mol. Biotech. 12:173-201. [0003] Compared to other antibody production systems, bacteria, particularly E. coli, provides many unique advantages. The raw materials used (i.e. bacterial cells) are inex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C07K16/00C07K16/22C07K16/28C07K16/32C07K16/42C12N1/21
CPCA61K2039/505C07K2317/52C07K16/22C07K16/2845C07K16/2878C07K16/2896C07K16/32C07K16/36C07K16/4291C07K2317/20C07K2317/41C07K2317/55C07K2319/00C12N15/70C07K16/00
Inventor SIMMONS, LAURAKLIMOWSKI, LAURAREILLY, DOROTHEAYANSURA, DANIEL
Owner GENENTECH INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com