Microfluidic chip system integrating cell sorting and detection

Abstract

The invention discloses a microfluidic chip system integrating cell sorting and detection. The system comprises a microfluidic chip, a high-frequency lock-in amplifier and a processor, wherein the microfluidic chip is formed by aligning and packaging a flow channel layer, an electrode layer, a substrate layer and a PCB (printed circuit board) in sequence; a cell sorting spiral flow channel, a detection main flow channel and a retraction flow channel are formed in the flow channel layer; the retraction flow channel is aligned to a planar metal electrode of the electrode layer; liquid electrode structures are formed on two sides of the main flow channel; an electrode of the electrode layer forms a differential high-frequency impedance measurement circuit together with the high-frequency lock-in amplifier through a power amplification circuit and an I / V conversion circuit of the PCB to realize differential detection of cell alternating-current impedance. The system can realize the integration of rare cell sorting and representation function, improves the integrity and accuracy of a cell detection technology, and can be widely used in the fields of rare cell biological study, early disease diagnosis and treatment and the like.

Description

technical field [0001] The invention relates to the field of microfluidic chip and biological particle manipulation and detection, in particular to a microfluidic chip rare cell detection system integrating spiral channel inertial sorting technology and differential electrical impedance measurement technology. Background technique [0002] Capturing and detecting tiny and rare cells in body fluids such as blood and pleural effusion is helpful for early diagnosis of diseases, monitoring of patients' conditions, and personalized treatment. Currently, rare cell detection methods commonly used in clinical practice include immunocytochemistry, flow cytometry, fluorescence in situ hybridization, and reverse transcription polymerase chain reaction. These methods have their own advantages, but they all use biomolecular markers expressed by rare cells As the object of analysis, there are common disadvantages such as complex operation, large sample consumption, low detection efficienc...

AI Technical Summary

Problems solved by technology

However, the existing microfluidic impedance cytometer has a low integration level, usually only includes a single functional module of impedance measurement and characterization, does not involve pretreatment processes such as cell sorting and focusing, and cannot directly detect rare cells in body fluids. Limiting the application of rare cell detection methods in clinical diagnosis

And the measurement frequency of the existing microfluidic impedance cytometer usually only reaches 10 7 Hz level, capable of analyzing cell membrane capacitance and cytoplasmic conductivity, but unable to obtain internal structural information such as the nucleus

In addition, at present, researchers usually use commercial impedance analyzers, which limits the application range of cell detection systems, and is not easy to achieve miniaturization and portability.

Images