Cell screening device based on dielectrophoresis and screening method thereof

A screening device and a technology of dielectrophoresis, which are applied in cell dissociation methods, stress-stimulated microbial growth methods, animal cells, etc., can solve problems such as low integration and low capture rate, and achieve a high degree of automation, simple chip structure, Simple to use effects

Inactive Publication Date: 2018-09-28
DALIAN MARITIME UNIVERSITY
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  • Abstract
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Problems solved by technology

However, when the amount of micro-nanoparticles or cells required to be separated is very small, the dielectrophoretic separation method faces the defects of low integration and low capture rate.

Method used

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  • Cell screening device based on dielectrophoresis and screening method thereof
  • Cell screening device based on dielectrophoresis and screening method thereof
  • Cell screening device based on dielectrophoresis and screening method thereof

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with the accompanying drawings; the non-limiting embodiments described below can enable those of ordinary skill in the art to understand the present invention more fully, but do not limit the present invention in any way.

[0030] Such as Figure 1-4 As shown, a cell screening device based on dielectrophoresis, including an ITO glass substrate layer 1, a 3D electrode layer 4, and a PDMS cover layer 13;

[0031] The ITO glass base layer 1 is provided with two rows of electrodes, which are made by wet etching the ITO film on the ITO glass, including the ITO electrode I2 and the ITO electrode II3, which serve as the gap between the power line and the 3D electrode layer 4. wire;

[0032] The 3D electrode layer 4 is deposited on the ITO glass base layer 1 and connected to the ITO electrode of the ITO glass base layer 1; the PDMS cover layer 13 is bonded to the ITO glass base layer 1 on which the 3D electro...

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Abstract

The invention relates to a cell screening device based on dielectrophoresis, and belongs to the technical field of cell filtration and separation. The cell screening device based on dielectrophoresiscomprises an ITO glass substrate layer, a 3D electrode layer and a PDMS cover sheet layer. The 3D electrode layer is composed of two rows of electrodes, wherein each of the four small rectangular electrodes and a large rectangular electrode are arranged on both sides of a chip aisle so as to generate a non-uniform electric field. By a design of the counter electrode, the target micro-cells are subjected to a positive dielectric electrophoresis force by using a dielectric electrophoresis force, the non-target cells are subjected to negative dielectrophoresis force, the sample cells are subjected to 'sinusoid' flow in a separation aisle through the cell screening device based on dielectrophoresis, when the sample cells are close to the small rectangular electrodes, the target cells are subjected to a stronger positive dielectrophoresis force, which will flow out of the flow channel near the electrode, so that the target cells can be better screened out of the sample cells, and the high-efficiency separation of the cells is accomplished.

Description

technical field [0001] The invention belongs to the technical field of cell filtration and sorting, and relates to a dielectrophoretic effect-based cell multistage screening device and a screening method thereof. Background technique [0002] Microfluidic chips have gained more and more attention in various fields in recent years, such as chemical analysis, single cell analysis, medical diagnosis and tissue engineering. Its great advantages lie in simple and fast processing, shortened analysis time, high sensitivity for detecting small-capacity samples, and multi-functional integration. The manipulation and separation of biological particles or cells based on microfluidic chips has an important impact in the fields of biomedicine, clinical diagnosis, food bacteria detection, and environmental monitoring, and has thus been studied by many scholars. [0003] Particle or cell separation methods based on microfluidics usually include optical, magnetic field, acoustic field, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12N5/09
CPCC12M47/04C12N5/0693C12N2509/00
Inventor 王俊生姜宗
Owner DALIAN MARITIME UNIVERSITY
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