Method for improving tolerance and increasing degradation rate of chlorella on phenol

A chlorella, degradation rate technology, applied in the methods of using microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of poor initial inoculation density phenol tolerance and low degradation rate, etc. Poor receptivity, low degradation rate, and shortened time

Active Publication Date: 2016-01-06
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a method for improving the tolerance and degradation rate of chlorella to phenol, which i...

Method used

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  • Method for improving tolerance and increasing degradation rate of chlorella on phenol
  • Method for improving tolerance and increasing degradation rate of chlorella on phenol
  • Method for improving tolerance and increasing degradation rate of chlorella on phenol

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Experimental program
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Embodiment 1

[0043] This embodiment takes Chlorella sp. with an initial non-degradable concentration of 500 mg / L phenol as an example to illustrate the feasibility of the technical solution.

[0044] The basal medium of Chlorella is the above-mentioned TAP liquid medium, culture conditions: initial pH=6.0, temperature 29±0.8°C, light intensity 117.67±6.89 μmol / m 2 / s, continuous light, 150rpm shaker shaking culture, the phenol concentration in the culture solution is 500mg / L.

[0045] In the first generation, Chlorella was inoculated in 100mL TAP medium (250mL Erlenmeyer flask) with an initial inoculation density of 0.6g / L containing phenol concentration of 500mg / L, and its dry weight and liquid medium were measured after 3 days of cultivation. The remaining phenol concentration.

[0046] Then, the chlorella of the first generation after being cultivated for 3 days was diluted and continued to be inoculated with an initial inoculation density of 0.6g / L in 100mL TAP liquid medium (the medi...

Embodiment 2

[0062] The basal medium of Chlorella is the above-mentioned TAP liquid medium, culture conditions: initial pH=6.0, temperature is 25°C, light intensity is 100 μmol / m 2 / s, continuous light, 100rpm shaker shaking culture, the phenol concentration in the culture solution is 450mg / L.

[0063] In the first generation, Chlorella was inoculated in 100mL TAP medium (250mL Erlenmeyer shake flask) containing 450mg / L phenol concentration at an initial inoculation density of 0.8g / L, and its dry weight and liquid medium were measured after 2 days of cultivation. The remaining phenol concentration.

[0064]Then, the chlorella of the first generation after being cultivated for 2 days was diluted and continued to be inoculated with an initial inoculation density of 0.8g / L in 100mL TAP liquid medium containing phenol concentration of 450mg / L (the medium was newly prepared) and cultivated for 2 days to measure its Dry weight and remaining phenol concentration in culture medium. Repeat above-...

Embodiment 3

[0066] The basal medium of Chlorella is the above-mentioned TAP liquid medium, culture conditions: initial pH=7.0, temperature is 35°C, light intensity is 200 μmol / m 2 / s, continuous light, 200rpm shaker shaking culture, the phenol concentration in the culture solution is 700mg / L.

[0067] In the first generation, Chlorella was inoculated in 100mL TAP medium (250mL Erlenmeyer shake flask) with an initial inoculation density of 1.0g / L containing phenol concentration of 700mg / L, and its dry weight and liquid medium were measured after 5 days of cultivation. The remaining phenol concentration.

[0068] Then, the chlorella of the first generation after being cultivated for 5 days was diluted and continued to be inoculated with an initial inoculation density of 1.0g / L in 100mL TAP liquid medium containing phenol concentration of 700mg / L (the medium was newly prepared) and cultivated for 5 days to measure its Dry weight and remaining phenol concentration in culture medium. Repeat ...

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Abstract

The invention provides a method for improving tolerance and increasing the degradation rate of chlorella on phenol. The method comprises the steps that firstly, the chlorella in a logarithmic phase is selected and subjected to shaking culture in a culture medium containing the phenol at certain initial density under the conditions of a certain temperature and illumination, wherein set culture time serves as an adaptive evolution cycle; secondly, the chlorella evolved through one cycle is diluted, the chlorella with the same initial density as that in the last step is taken and subjected to shaking culture in another culture medium under the same conditions for the same time; the culture process is repeated until the growing speed of the chlorella and the degradation rate of the chlorella on the phenol tend to be stable. According to the method, the chlorella is subjected to adaptive evolution experiments, the growing speed of the chlorella and the degradation rate on phenol are increased, and the tolerance on phenol is improved; meanwhile, the initial inoculum density is reduced, the time needed for completely degrading the high-concentration phenol is shortened, wastewater treatment cost is reduced, and superior chlorella is provided for treating industrial wastewater.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a method that can not only improve the tolerance of chlorella to high-concentration phenol, but also improve the degradation rate of chlorella to high-concentration phenol. Background technique [0002] Water is the source of life. However, with the development of the economy and the improvement of people's living standards, industries such as metallurgy, chemical industry, electroplating, papermaking, printing and dyeing, and leather making have developed rapidly, and the discharge of various toxic organic substances has increased. Phenolic compounds mainly exist in industrial wastewater such as oil refining, gas washing, coking, papermaking, synthetic ammonia, wood preservation, petrochemical, chemical, pharmaceutical, paint, coating, plastic pesticides, etc. Phenol is a highly toxic substance that can enter the human body through skin contact or through the mouth. It co...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12R1/89
Inventor 孙予罕王丽波赵权宇汪靓
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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