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High-temperature resistant yeast capable of producing xylitol and application thereof

A technology of xylitol and high temperature resistance, which is applied in the field of biotransformation of xylose to produce xylitol and high temperature resistant yeast. It can solve the problems of high energy consumption, high separation cost and high equipment requirements, and achieve low environmental pollution and high product quality. Reliable quality and good product quality

Inactive Publication Date: 2013-07-03
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method needs to concentrate and crystallize the corncob hydrolyzate to obtain xylose, then re-dissolve and dilute it, and then concentrate and crystallize it after hydrogenation, which requires high energy consumption
In addition, chemical methods use metal powder catalysts to separate products, especially the production of food-grade xylitol, which brings high separation costs, and the products have certain risks when used in food.
There is also a chemical method that requires hydrogen production, and hydrogenation is carried out under high temperature and high pressure, requiring high equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Obtaining of Thermotolerant Candida maltosa (Candida maltosa) TIB-Y1020 CGMCCNo5521

[0019] Self-isolated Candida maltosa (Candida maltosa) was inoculated in the adaptive evolution medium, which had a pH of 5.5 and contained the following components in mass percent: xylose 10%, beef extract 3%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O0.05%. After culturing at 37°C and 220r / min for 12 hours in a 50ml shake flask containing 10mL of medium, it was then inoculated in a fresh same medium with an OD600 value of 0.2, and subcultured in the same way. By analogy, after adapting the strain to a stable growth environment at 37°C, use the same medium and culture conditions to gradually increase the growth temperature, and carry out adaptive acclimation until the temperature reaches 40°C, so that it can still grow stably, and the growth rate is the same as the starting point. The growth amount of the strains was basically the same at 30° C., thereby obtaining Candida maltosa TI...

Embodiment 2

[0020] Example 2 Comparison of xylitol production between Candida maltosa (Candida maltosa) TIB-Y1020CGMCC No552130°C and 40°C

[0021] Candida maltosa (Candida maltosa) TIB-Y1020CGMCC No5521 was inoculated in the seed medium, and the pH of the medium was 5.5, containing the following components in mass percent: xylose 5%, peptone 1%, yeast extract 1%, in After culturing at 220r / min at 30°C and 40°C for 24 hours in a 250ml shake flask containing 20mL, centrifuge at 2000r / min to collect the bacteria, resuspend the bacteria in a 20mL, 20% xylose solution in a 250mL shake flask middle. Bacteria cultured at 30°C continued to be cultured at 30°C and 40°C for 36-48 hours at 40°C and 220r / min, and the xylose consumption and xylitol in the reaction solution were measured by high performance liquid phase method generation. Candida maltosa TIB-Y1020CGMCC No5521 needs 48 hours to consume xylose at 30°C to produce 160 g / L xylitol, while at 40°C it only takes 40 hours to consume xylose a...

Embodiment 3

[0022] Example 3 Transformation of Candida maltosa (Candida maltosa) TIB-Y1020CGMCC No5521 at 40°C to produce xylitol

[0023] Candida maltosa TIB-Y1020CGMCC No5521 was inoculated in a 500mL shake flask with 25mL of cell culture medium, the pH of the medium was 5.4, and it contained the following components in mass percentage: xylose 3%, peptone 1%, yeast extract 0.5 %. After culturing at 40° C. and 220 r / min for 16 hours, the cells were collected by centrifugation, and the cells were resuspended in a 250 mL shake flask containing 25 mL of xylose mother liquor containing 20% ​​xylose. Culture was carried out at 40° C. and 220 r / min, and xylose consumption and xylitol production in the reaction solution were measured by high performance liquid phase method. Candida maltosa TIB-Y1020CGMCC No5521 can produce 169g / L xylitol in 48 hours. Continue to centrifuge and recover the thalli to carry out 9 rounds of converting xylose to produce xylitol, and the xylitol output can be maint...

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PUM

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Abstract

Xylitol is an important functional food additive and chemical raw material, and has extensive applications. According to the invention, a high-temperature adaptive evolution method is utilized to screen TIB-Y1020 of high-temperature resistant Candida maltose which is CGMCC (china general microbiological culture collection center) No. 5521 and can grow well at the temperature ranging from 38 DEG C to 42 DEG C without influencing the transformation production of the xylitol. The high-temperature resistance strain can reduce the manufacturing cost increase brought by temperature reduction, thereby having an extensive industrial application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a high-temperature-resistant yeast strain producing xylitol, Candida maltosa (Candida maltosa) TIB-Y1020CGMCC No5521 and a method for producing xylitol by biotransforming xylose with the fungus. Background technique [0002] Xylitol is a natural five-carbon polyol and one of the important functional food additives. The sweetness of xylitol is 1.05 times that of sucrose, and its metabolism does not require insulin. It can replace sucrose as a sweetener, nutrient and medicine for diabetic patients. In recent years, the market demand for xylitol has been continuously expanding. At present, the world market for xylitol is estimated at 360 million US dollars, and the annual output value of xylitol in China has exceeded 1 billion yuan. In addition, xylitol is widely used in pharmaceutical, chemical, food, paint, leather and other industries. Xylitol is also a platform chemical...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/18C12R1/72
Inventor 王钦宏耿笑林张国强涂然贺鹏江宁
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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