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A method for improving the tolerance and degradation rate of chlorella to phenol

A chlorella, degradation rate technology, applied in microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc., can solve the problems of poor initial inoculation density phenol tolerance and low degradation rate, etc. Poor receptivity, low degradation rate, improved tolerance and degradation rate

Active Publication Date: 2019-02-01
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a method for improving the tolerance and degradation rate of chlorella to phenol, which is used to solve the problem that the initial inoculation density of chlorella in the prior art is too high and Chlorella has poor tolerance to high concentrations of phenol and the problem of low degradation rate

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  • A method for improving the tolerance and degradation rate of chlorella to phenol
  • A method for improving the tolerance and degradation rate of chlorella to phenol
  • A method for improving the tolerance and degradation rate of chlorella to phenol

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Embodiment 1

[0043] In this embodiment, Chlorella sp. whose initial concentration of 500 mg / L phenol cannot be degraded is taken as an example to illustrate the feasibility of the technical solution.

[0044] The basic medium of Chlorella is the above-mentioned TAP liquid medium, and the culture conditions: initial pH=6.0, temperature 29±0.8°C, light intensity 117.67±6.89μmol / m 2 / s, continuous light, 150rpm shaker shaking culture, the phenol concentration in the culture solution is 500mg / L.

[0045] The first generation of Chlorella was inoculated in 100mL TAP medium (250mL flask) with a phenol concentration of 500mg / L at an initial inoculation density of 0.6g / L. After 3 days of cultivation, the dry weight and liquid medium were measured. The remaining phenol concentration.

[0046] Next, dilute the first generation chlorella after 3 days of culture and continue to inoculate it with an initial inoculation density of 0.6 g / L in 100 mL of TAP liquid medium containing 500 mg / L of phenol (the medium...

Embodiment 2

[0062] The basal medium of Chlorella is the above-mentioned TAP liquid medium, and the culture conditions: initial pH=6.0, temperature 25℃, light intensity 100μmol / m 2 / s, continuous light, 100rpm shaker shaking culture, the phenol concentration in the culture solution is 450mg / L.

[0063] The first generation of Chlorella was inoculated in 100 mL of TAP medium (250 mL flask) with a phenol concentration of 450 mg / L at an initial inoculation density of 0.8 g / L, and measured its dry weight and liquid medium after 2 days of cultivation The remaining phenol concentration.

[0064] Next, dilute the first generation Chlorella after 2 days of culture and continue to inoculate it with an initial inoculation density of 0.8 g / L in 100 mL of TAP liquid medium containing 450 mg / L of phenol (the medium is re-formulated), and then culture for 2 days. Dry weight and concentration of phenol remaining in the culture medium. Repeat the above process and carry out 40 cycles of cyclic culture. The dr...

Embodiment 3

[0066] The basal medium of Chlorella is the above-mentioned TAP liquid medium, and the culture conditions: initial pH=7.0, temperature 35℃, light intensity 200μmol / m 2 / s, continuous light, 200rpm shaker shaking culture, the phenol concentration in the culture solution is 700mg / L.

[0067] The first generation of Chlorella was inoculated into 100mL TAP medium (250mL flask) with a phenol concentration of 700mg / L at an initial inoculation density of 1.0g / L. After 5 days of cultivation, the dry weight and liquid medium were measured. The remaining phenol concentration.

[0068] Next, dilute the first generation Chlorella after 5 days of cultivation and continue to inoculate it with an initial inoculation density of 1.0 g / L in 100 mL of TAP liquid medium containing 700 mg / L of phenol (the medium is re-prepared). Dry weight and concentration of phenol remaining in the culture liquid medium. Repeat the above process and carry out 50 cycles of cyclic culture. The dry weight of chlorella ...

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Abstract

The invention provides a method for improving tolerance and increasing the degradation rate of chlorella on phenol. The method comprises the steps that firstly, the chlorella in a logarithmic phase is selected and subjected to shaking culture in a culture medium containing the phenol at certain initial density under the conditions of a certain temperature and illumination, wherein set culture time serves as an adaptive evolution cycle; secondly, the chlorella evolved through one cycle is diluted, the chlorella with the same initial density as that in the last step is taken and subjected to shaking culture in another culture medium under the same conditions for the same time; the culture process is repeated until the growing speed of the chlorella and the degradation rate of the chlorella on the phenol tend to be stable. According to the method, the chlorella is subjected to adaptive evolution experiments, the growing speed of the chlorella and the degradation rate on phenol are increased, and the tolerance on phenol is improved; meanwhile, the initial inoculum density is reduced, the time needed for completely degrading the high-concentration phenol is shortened, wastewater treatment cost is reduced, and superior chlorella is provided for treating industrial wastewater.

Description

Technical field [0001] The present invention relates to the field of microalgae biotechnology, in particular to a method that can improve the tolerance of chlorella to high-concentration phenol and the degradation rate of chlorella to high-concentration phenol. Background technique [0002] Water is the source of life. However, with the development of economy, people's living standards are improving day by day, and industries such as metallurgy, chemical industry, electroplating, papermaking, printing and dyeing, and leather making are developing rapidly, and the discharge of various toxic organic substances is increasing. Phenolic compounds mainly exist in industrial wastewater such as oil refining, gas washing, coking, papermaking, synthetic ammonia, wood preservation, petrochemicals, chemistry, pharmaceuticals, paints, coatings, plastics and pesticides. Phenol is a highly toxic substance that can enter the human body through skin contact or through the oral cavity. It will con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36C12R1/89
Inventor 孙予罕王丽波赵权宇汪靓
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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