Algae species breeding method of microalgae capable of tolerating high-concentration flue gas

A technology for microalgae and algae species, which is applied in the methods of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of large screening workload and low efficiency, and achieve high sugar content and high efficiency. , the effect of less workload

Active Publication Date: 2017-05-17
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

Direct screening from more than 40,000 kinds of micr

Method used

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  • Algae species breeding method of microalgae capable of tolerating high-concentration flue gas
  • Algae species breeding method of microalgae capable of tolerating high-concentration flue gas
  • Algae species breeding method of microalgae capable of tolerating high-concentration flue gas

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Experimental program
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Effect test

Embodiment 1

[0041] Purchase a strain of Chlorella vulgaris FACHB-481 (hereinafter referred to as the original strain) from the freshwater algae stock. The algae strain was exposed to gradually increasing concentrations of flue gas (see figure 1 ) under 46 cycles of evolution to obtain Chlorella Cv, and the medium is BG11 medium.

[0042] Adaptive evolution culture conditions: the initial inoculation density of microalgae is 0.35g / L, the temperature is 28°C; the apparent ventilation rate is 0.3vvm; the light intensity is about 100μmol / m 2 / s, continuous light; the Cv ventilation method is specifically the flue gas ventilation on the first day of each cycle, and 10% CO in the remaining two days 2 Ventilation; the culture system is 350ml. The initial acclimation gas concentration is CO 2 10%+NOx50ppm+SO 2 25ppm, the evolution time of each cycle was set to 3 days, and each group of experiments was set up with 3 parallels. When the chlorella died, the surviving parallel group of chlorell...

Embodiment 2

[0048] A Scenedesmus strain FACHB-489 was cultivated, and the continuous aeration mode was adaptively evolved for 200 cycles, and the medium was BG11.

[0049] Adaptive evolution culture conditions: the initial inoculation density of microalgae is 0.2g / L, the temperature is 20°C; the apparent ventilation rate is 0.1L / min; the light intensity is about 50μmol / m 2 / s, continuous lighting. Continuous flue gas ventilation, the culture system is 350ml, and the initial acclimation gas concentration is 5% CO 2 +40ppmNOx+20ppmSO 2 . The evolution time of each cycle is set to 2 days, the flue gas is passed on the first day, and 10% CO is passed on the second day 2 . After 40 cycles of culture, increase the flue gas concentration by 1% CO 2 , 40ppm NOx, 20ppmSO 2 And cultured for 40 cycles. Until the flue gas concentration is 10% CO 2 , 200ppm NOx and 100ppm SO 2 . The evaluation test proved the improvement of the tolerance of Scenedesmus to flue gas before and after domesticat...

Embodiment 3

[0051] Nannochloropsis (GY-H14, Shanghai Guangyu Biotechnology Co., Ltd.) was evolved for 45 cycles of intermittent flue gas aeration under gradually increasing flue gas concentration to obtain domesticated strains. The medium is TAP medium.

[0052] Adaptive evolution culture conditions: the initial inoculation density of microalgae is 0.5g / L, the temperature is 35°C; the apparent ventilation rate is 0.5vvm; the light intensity is about 500μmol / m 2 / s, continuous lighting. The initial acclimation gas concentration is CO 2 10%+NOx 50ppm+SO 2 25ppm, the evolution time of each cycle was set to 5 days, and each experiment set 3 parallels. The ventilation method is specifically flue gas for the first 2 days of each cycle, and 10% CO for the remaining 3 days 2 . Each stage was cultivated for 15 cycles, and then the flue gas concentration was increased to 0% CO 2 , 50ppm NOx, 25ppm SO 2 , and cultivated for another 15 cycles, and gradually increased until the concentration o...

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Abstract

The invention belongs to the technical field of microalgae organisms, and in particular relates to an algae species breeding method of microalgae capable of tolerating high-concentration flue gas. The algae species breeding method refers to the highest emission flue gas concentration allowed by the national standard as an environmental stress condition, adopts a strategy of gradually increasing the flue gas concentration, and enhances the tolerance of the microalgae to the flue gas by activating adaptive evolution, so as to carry out biological fixation on the flue gas. Before the adaptive evolution, the best growth flue gas concentration of the microalgae is about CO2 2%+NOx 0ppm+SO2 0ppm; when the flue gas concentration is CO2 10%+NOx 200ppm+SO2 100ppm, the microalgae cannot survive obviously. By means of the adaptive evolution of the breeding method, the obtained microalgae can tolerate CO2 10%+NOx 200ppm+SO2 100ppm and maintain a more biomass accumulation, and is high in sugar content. Compared with large-scale screening, the breeding method provided by the invention has the advantages of less amount of work, definite purpose and high efficiency.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for breeding microalgae species that can tolerate high-concentration flue gas. Background technique [0002] my country is rich in coal resources, but the distribution is significantly different. The energy consumption structure will still be dominated by fossil energy such as coal. According to the 2013 Statistical Bulletin on National Economic and Social Development, my country produced 3.68 billion tons of raw coal in 2013, imported 327 million tons of raw coal, and consumed nearly 4 billion tons in total. A large amount of coal burning ensures rapid economic development, but it also brings serious environmental problems, such as greenhouse gas problems, which are gradually affecting the climate on which we live. In order to cope with the pressure of carbon emissions from the international community, in 2009 my country formally promised that by 20...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/36C12R1/89
CPCC12N1/12C12N1/36
Inventor 赵权宇程度佳汪靓魏伟孙予罕
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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