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Efficient breeding method of ultrahigh-concentration beer yeast strain

A technology of brewer's yeast and brewer's yeast, which is applied to beer fermentation methods, beer brewing, microorganism-based methods, etc., can solve the problems of cumbersome steps, long fermentation period, low screening efficiency, etc., and achieves efficient screening process and screening process. simple steps

Active Publication Date: 2020-09-11
TSINGTAO BREWERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of long fermentation period, cumbersome steps and low screening efficiency in the prior art, the present invention proposes a high-efficiency breeding method for ultra-high-concentration beer yeast strains with simple operation, high screening efficiency and good performance

Method used

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  • Efficient breeding method of ultrahigh-concentration beer yeast strain
  • Efficient breeding method of ultrahigh-concentration beer yeast strain
  • Efficient breeding method of ultrahigh-concentration beer yeast strain

Examples

Experimental program
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Effect test

Embodiment 1

[0075] The present embodiment provides a kind of high-efficiency breeding method of extra-high-concentration brewer's yeast strain, and the specific method is:

[0076] ·Cultivation medium formula:

[0077] YPD solid medium (adding ethanol and sorbitol): yeast extract 1%, peptone 2%, maltose 1%, absolute ethanol 10%, sorbitol 30%, agar 2%;

[0078] YPD liquid medium: yeast extract 1%, peptone 2%, maltose 1%;

[0079] Primary screening medium: yeast extract 1%, peptone 2%, maltose 1%, absolute ethanol 10%, sorbitol 30%, 2-deoxy-D-glucose 0.05%, agar 2%;

[0080] Wort medium: the concentration of extra-high concentration wort is 32°P, and the addition of absolute ethanol is gradually increased from 6% to 16%.

[0081] ·Experimental equipment: constant temperature incubator, automatic high-throughput microbial droplet culture instrument, etc.

[0082] ·experimental method:

[0083] (1) The candidate bacterial strain is coated on the YPD solid medium plate that adds ethanol an...

Embodiment 2

[0090] The present embodiment utilizes the selection method that embodiment 1 provides to screen and obtain 10 strains of rescreening bacterial strains and carry out EBC fermentation experiment, and its specific method is as follows:

[0091] Utilize the screening method provided in the embodiment of the present invention 1 to screen and obtain 10 rescreened strains, and the strain numbers are respectively 8#, 10#, 11#, 21#, 38#, 39#, 46#, 55#, 61#, 4 -3#, the above yeast strains were subjected to EBC tube fermentation test in 32°P ultra-high concentration wort, and the yeast strains suitable for ultra-high concentration brewing were screened out by evaluating the fermentation index and maturity index of the above yeast strains. Among them, the experimental operation and experimental results of EBC tube fermentation are as follows:

[0092] (1) Experimental materials and methods:

[0093] ·Experimental group:

[0094] Experimental group: 8#, 10#, 11#, 21#, 38#, 39#, 46#, 55#, ...

Embodiment 3

[0110] The present embodiment utilizes the related results obtained in Example 2 to carry out the genetic stability experiment on the re-screened bacterial strain, and its specific method is as follows:

[0111] ·experimental method:

[0112] According to a series of results obtained in Example 2, according to indicators such as degree of fermentation, beer maturity (diacetyl and acetaldehyde content in beer), the numbering 21# and 61# yeast strains were selected to be subcultured and domesticated to verify their genetic stability. The specific method is: 21#, 61# yeast strains are subcultured in 32 ° P extra-high concentration wort, and after passing on for 10 generations, the yeast after subculture and the original yeast are carried out to the EBC tube fermentation test (the experimental method is the same as that of Example 2) , and compare the fermentation performance indicators of the two yeast strains before and after passage.

[0113] ·Experimental results:

[0114] C...

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Abstract

The invention provides an efficient breeding method of an ultrahigh-concentration beer yeast strain, and belongs to the field of strain breeding. The breeding method comprises the steps of screening original strains, screening primarily screened strains, culturing the adaptability of the primarily screened strains, evaluating and testing the strains and the like. The ultrahigh-concentration beer yeast strain TG-01 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.19839 is screened by utilizing the efficient breeding method provided by the invention, and is preserved in China General Microbiological Culture Collection Center on May 26, 2020. According to the method disclosed by the invention, by establishing a method of combining specific stress condition efficient screening and adaptive evolution screening, 2-deoxy-D-glucose, sorbitol and alcohol are combined together to simulate a stress condition higher than an ultrahigh-concentrationfermentation environment of mass production, and the target strain which is insensitive to glucose, resistant to hypertonic and resistant to alcohol can be further screened out directionally and efficiently. The method has a very wide application prospect in the field of strain breeding.

Description

technical field [0001] The invention belongs to the field of strain breeding, and in particular relates to a high-efficiency breeding method for ultra-high-concentration beer yeast strains. Background technique [0002] Ultra-high-concentration brewing technology is a new trend in the development of the beer industry at home and abroad, which can greatly reduce energy consumption and improve equipment utilization, and the higher the wort concentration, the more prominent the advantages. Compared with high-concentration brewing, ultra-high-concentration brewing (≥22°P) puts forward higher technical requirements and difficulties for yeast strains, such as ultra-high-concentration wort, high osmotic pressure and other environments will affect the normal physiological and biochemical characteristics of yeast , reduce yeast activity and fermentation performance, and ultimately affect the flavor and coordination of beer. Therefore, the premise of realizing ultra-concentrated brew...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/18C12C11/02C12R1/85
CPCC12N1/36C12N1/18C12C11/02C12R2001/85C12N1/185
Inventor 尹花董建军余俊红陈璐万秀娟赵玉祥陈嵘侯晓平贺扬
Owner TSINGTAO BREWERY
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