Adaptive evolution method for gluconobacter oxydans efficiently utilizing non-glucose carbon sources and application of adaptive evolution method

A technology of Gluconobacter oxydans and adaptive evolution, applied in the fields of biotechnology and microorganisms, can solve the problems of affecting the production efficiency of sugar acid, low utilization efficiency, etc.
CN111139211AActive Publication Date: 2020-05-12SHANXI SYNTHETIC BIOLOGY INST CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANXI SYNTHETIC BIOLOGY INST CO LTD
Publication Date
2020-05-12

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Abstract

The invention discloses an adaptive evolution method for gluconobacter oxydans efficiently utilizing non-glucose carbon sources and application of the adaptive evolution method, and belongs to the biotechnology and microbial technology field. The method comprises the concrete steps that firstly, the gluconobacter oxydans is cultured in a hydrolysate containing inhibitor lignocellulose, then the cultured gluconobacter oxydans is transferred into a hydrolysate without the inhibitor for further culture, and the gluconobacter oxydans is alternately transferred in such a way until the fermentationperformance of a strain reaches stable. The new strain obtained by acclimatization has obviously improved utilization efficiency of the non-glucose carbon sources, and the strain is named as gluconobacter oxydans RM7, and the collection number of the strain is CGMCC No.14801; and the method is simple and efficient, and has important referential significance in breeding of strains containing an inhibitor lignocellulose system.
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Description

【Technical field】

[0001] The invention relates to the fields of biotechnology and microorganism technology, and relates to a method for the adaptive evolution of Gluconobacter oxydans that efficiently utilizes non-glucose carbon sources and its application. 【Background technique】

[0002] Lignocellulosic raw materials are composed of cellulose, hemicellulose and lignin. Among them, cellulose can be hydrolyzed into glucose by cellulase, and hemicellulose can be degraded into xylose, mannose and galactose by acid pretreatment or under the action of hemicellulase. The hemicellulose-derived sugars account for about 40% of the total sugars. Common strains can only use glucose and cause a large loss of other fermentable sugars. These unused sugars also increase the COD content in the process wastewater, resulting in a more severe sewage treatment load.

[0003] Gluconobacter oxydans has a unique redox ability to convert various sugars into corresponding sugar acids. Its membran...

Claims

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