Gene and application thereof
A gene and nucleotide sequence technology, applied in the biological field, can solve problems such as the lack of records of important genes
Inactive Publication Date: 2016-04-20
BEE RES INST CHINESE ACAD OF AGRI SCI
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Problems solved by technology
[0003] The discovery of native western honeybees in China has had a great impact at home and abroad, but the important genes that adapt to the local environment of black bees in the western regions have not yet been recorded
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Embodiment 1
[0040] (1) Collect bee samples and extract DNA. Samples with a DNAOD value of 1.8-2.0 and a content greater than 1.5 micrograms are considered qualified;
[0041](2) Qualified DNA sample library construction:
[0042] Qualified DNA samples were randomly fragmented into 350bp fragments by a Covaris shredder. TruSeqLibraryConstructionKit was used to build the library, and the reagents and consumables recommended in the instructions were strictly used. The DNA fragments undergo steps such as end repair, tailing, sequencing adapters, purification, and PCR amplification to complete the entire library preparation. The constructed library was sequenced by illuminaHiSeq.
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The invention relates to the biotechnology field, in particular to a gene and application thereof. Whole genome sequencing is adopted for carrying out whole genome re-sequencing on a large number of western black bee individuals, and the special gene of western black bees is obtained. The gene has the important role in differentiation of the western black bees and bees in other regions and the adaptive evolution of the western black bees for the local environment. The Foxo gene or the Ebony gene can be used for identifying the western black bees and other species, and can also be used for researching the species resource diversity of bees and researching cold-resist genes. The research gap of researchers in China in the western black bee field can be filled.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to genes and applications thereof. Background technique [0002] my country has a long history of bee breeding and rich bee germplasm resources, but for a long time, only Oriental honeybees have been found in my country, and there is no native Western honeybee. The collection ability of Eastern honeybees is slightly inferior to that of Western honeybees. With the introduction of Western honeybees, more and more Chinese beekeepers began to raise Western honeybees. The germplasm resources of native bees in my country are threatened. The imported resources pose a threat to the local groups in our country; at the same time, the imported resources also have a certain degree of inadaptability to the local environment. It is of great significance to the germplasm resources of honey bees in our country if we can find the native Western honeybee in our country. Mr. Shi Wei, director of the Chi...
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IPC IPC(8): C07K14/435C12N15/12C12N15/63C12N15/11C12Q1/68
CPCC07K14/43572C12Q1/6888C12Q2600/124C12Q2600/156A01K67/033
Inventor 陈超石巍刘之光陈晓王慧华郭海坤
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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