Extraction method of buckwheat protein from buckwheat bran
A technology of buckwheat bran and buckwheat, applied in plant protein processing, etc., can solve the problems of poor product quality, low extraction rate, low purity, etc., and achieve the effects of ensuring quality, increasing content and yield, and improving mass transfer characteristics
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Embodiment 1
[0013] Take 2.5kg of tartary buckwheat bran and add 0.075L of clear water to make the water content of the bran 13%. Extrude and expand with a twin-screw extruder. °C, 160 °C, screw speed 200r / min. Add 25L of NaOH solution with pH=9 into the puffed tartary buckwheat bran, stir and extract at 40°C for 25min, centrifuge at 4500r / min for 5min, and collect supernatant and precipitate respectively. The precipitate was extracted again by repeating the above steps. Combine the supernatants from the two centrifuges, add 10% hydrochloric acid to adjust the pH value to 4.3-4.5, let it stand overnight, and centrifuge (4500r / min, 5min). The precipitate was washed with distilled water until neutral, dried in vacuum at 60°C, and pulverized. 450g of tartary buckwheat protein powder was obtained, and the crude protein content of the product measured by Kjeldahl method was 74.2%, and the protein yield was 74.1%.
Embodiment 2
[0015] Get sweet buckwheat bran 2.5kg, experimental method and condition are the same as embodiment 1, the result obtains sweet buckwheat protein powder 445g, and Kjeldahl method measures product crude protein content 72.3%, protein yield 71.5%.
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