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Method for preparing agrocybe cylindracea hydrolysate by using compound enzyme method

A technology of mushroom hydrolyzate and compound enzyme method, which is applied in the field of preparation of tea tree mushroom hydrolyzate by compound enzyme method, can solve the problems of low extraction efficiency and low enzyme activity, and achieve the effects of improving extraction rate, reducing operation steps and reducing production cost

Inactive Publication Date: 2011-09-28
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] According to the search, the existing extraction methods include a single extraction method and several combination methods composed of a single extraction method to extract tea tree mushroom polysaccharides, but the extraction efficiency of the current extraction methods is low, and the enzyme activity after extraction is not high

Method used

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  • Method for preparing agrocybe cylindracea hydrolysate by using compound enzyme method

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Embodiment 1

[0028] A method for preparing tea tree mushroom hydrolyzate by compound enzyme method, the steps are as follows:

[0029] (1) Wash 1 kg of dried tea tree mushroom, crush it into 80 meshes, add water to make slurry according to the weight ratio of tea tree mushroom and water as 1:2, boil for 15 minutes to sterilize, and obtain slurry;

[0030] (2) The first enzymatic hydrolysis: first, add water to adjust the concentration of the slurry, so that the ratio of the weight of the dry tea tree mushroom raw material to water is 1:20 (kg:L), add 20L water to 1kg of tea tree mushroom, and then use food grade Adjust the pH value with citric acid or dilute hydrochloric acid to keep the pH value at 4.5. Then, add compound enzyme I and perform enzymatic hydrolysis for 3 hours at 45-55°C. The temperature can be adjusted with a water bath heater, and the enzymatic hydrolysis is stirred at a slow speed. Carry out under the state, obtain the enzymolysis solution for the first time;

[0031] A...

Embodiment 2

[0040] A method for preparing tea tree mushroom hydrolyzate by compound enzyme method, the steps are as follows:

[0041] (1) Wash 2 kg of dried tea tree mushroom, crush it, add water to adjust the slurry according to the weight ratio of tea tree mushroom and water as 1:5, boil for 15 minutes to sterilize, and obtain a slurry;

[0042] (2) The first enzymatic hydrolysis: First, add water to adjust the concentration of the slurry, so that the ratio of the weight of the dry tea tree mushroom raw material to water is 1:20 (kg:L), add 40L water to 1kg of tea tree mushroom, and then use food grade Adjust the pH value with citric acid or dilute hydrochloric acid to keep the pH value at 5.0. Then, add compound enzyme I and perform enzymolysis at 50°C for 3 hours. The temperature can be adjusted with a water bath heater, and the enzymolysis is under slow stirring. Carry out to obtain the first enzymolysis solution;

[0043]Composite enzyme I is made up of cellulase 3g, xylanase 3g, β...

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Abstract

The invention relates a method of for preparing agrocybe cylindracea hydrolysate by using a compound enzyme method, comprising the following steps of: (1) washing agrocybe cylindracea, crushing and pulping; (2) adding water for mixing pulp according to the proportion that the agrocybe cylindracea to water is 1:2 to 1:5; (3) first enzymolysis: adding compound enzyme I which comprises cellulase, xylanase, beta-dextranase, mannase and chitinase, wherein the addition amount of the compound enzyme I is 0.05% to 0.3% of that of the agrocybe cylindracea, and carrying out enzymolysis for 2-4 hours at 45 DEC C to 55 DEG C; (4) second enzymolysis: adding compound enzyme II and carrying out enzymolysis for 2-4 hours at 50 DEC C to 60 DEG C; (5) boiling enzymatic hydrolysate and killing enzyme; and (6) adsorbing, filtering and concentrating to obtain transparent hydrolysate. In the method, a compound enzyme preparation is used for preparing the agrocybe cylindracea so as to fully give play to the synergistic interaction; and a subsection processing technology is adopted for leading bioactive substances in the agrocybe cylindracea to be released to the greatest extent.

Description

technical field [0001] The invention belongs to the field of biological extraction, in particular to a method for preparing a tea tree mushroom hydrolyzate by a compound enzyme method. Background technique [0002] Tea Tree Mushroom (Agrocybe aegerita), also known as Agrocybe aegerita, is also called Agrocybe aegerita. "Mushroom", which belongs to Fungi Kingdom, Basidiomycota, Laminomycetes, Agaricaceae, Copromataceae, and Agaricaceae. It is commonly used in folks for the prevention and treatment of many common diseases and frequently-occurring diseases. After drying, it can treat dizziness, For headaches, vomiting, low-grade fever in children, asthma in the elderly, modern medical research has proved that it can nourish yin and strengthen yang, and has an ideal auxiliary treatment function for kidney deficiency, frequent urination, edema, cancer, high blood pressure, premature aging, low-grade fever in children, and bedwetting. These functions come from a variety of biolog...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 毛淑红路福平周瑶王娜华伯元王春霞
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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