Bacterial strain capable of producing chitinase and application of bacterial strain to production of chitinase by fermenting crab shell

A chitinase and strain technology, applied in the directions of enzymes, enzymes, bacteria, etc., can solve the problems of restricting the large-scale use of chitinase, high production cost of enzyme preparations, and insufficient activity to meet the needs of industrialization, and achieve significant economic benefits and Environmental benefits, low production costs, and efficient hydrolysis

Active Publication Date: 2015-03-25
广州增城潮徽生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because chitinase has high economic development value and broad application prospects, there are many reports about chitinase-producing microorganisms, but there are still enzyme prep...

Method used

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  • Bacterial strain capable of producing chitinase and application of bacterial strain to production of chitinase by fermenting crab shell
  • Bacterial strain capable of producing chitinase and application of bacterial strain to production of chitinase by fermenting crab shell
  • Bacterial strain capable of producing chitinase and application of bacterial strain to production of chitinase by fermenting crab shell

Examples

Experimental program
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Embodiment 1

[0034] A method for producing chitinase by fermentation of crab shells includes the following steps:

[0035] (1) Activation of Paenibacillus pasadenensis CS0611 Take the slant culture and place it in a 37°C constant temperature incubator for 30 minutes to activate the strain.

[0036] (2) Seed liquid culture

[0037] An appropriate amount of the slant culture was inoculated into the seed medium, and the seed medium was LB medium, which consisted of: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH 7.0. The culture conditions were 37°C, the rotating speed of the shaker was 180 rpm, and the culture time was 20 h.

[0038] (3) Liquid fermentation culture

[0039] Transfer the seed liquid to the fermentation medium with an inoculum of 4%. The fermentation medium consists of: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.08 g / L, potassium dihydrogen phosphate 0.02 g / L, and magnesium sulfate 0.04 g / L, sodium chloride 0.1g / L. The culture conditions were 30°C, the r...

Embodiment 2

[0044] The difference between this embodiment and Embodiment 1 is:

[0045] Liquid fermentation culture

[0046] The composition of the enzyme production medium is: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.07 g / L, potassium dihydrogen phosphate 0.03 g / L, magnesium sulfate 0.05 g / L, and sodium chloride 0.1 g / L. The inoculation amount is 6%, the culture condition is 30°C, the rotation speed of the shaker is 200 rpm, and the culture time is 48 hours.

[0047] After the fermentation is completed under the above conditions, the supernatant of the fermentation broth is centrifuged to obtain the crude chitinase enzyme solution, and the enzyme activity can reach 205.4mU / mL.

Embodiment 3

[0049] The difference between this embodiment and Embodiment 1 is:

[0050] Liquid fermentation culture

[0051] The composition of the enzyme production medium is: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.08 g / L, potassium dihydrogen phosphate 0.02 g / L, magnesium sulfate 0.04 g / L, and sodium chloride 0.1 g / L. The inoculum amount is 5%, the culture condition is 30°C, the rotation speed of the shaker is 180 rpm, and the culture time is 72 hours.

[0052] After the fermentation is completed under the above conditions, the supernatant of the fermentation broth is centrifuged to obtain the crude chitinase enzyme solution, and the enzyme activity can reach 200.2mU / mL.

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Abstract

The invention provides a bacterial strain capable of producing chitinase and application of the bacterial strain to production of chitinase by fermenting crab shell. The bacterial strain is paenibacillus pasadenensis CS0611 and is preserved in China Center for Type Culture Collection; the preservation number is CCTCC M2014458; the preservation date is October 8, 2014. The bacterium is a start strain; the crab shell is used as a carbon source and a nitrogen source and is fermented for producing chitinase. The fermentation technology is simple; the production cost is low; the enzyme activity of the fermentative supernatant liquid can reach 211.3mU/mL; the wastes of shrimp and crab shells can be efficiently hydrolyzed; the pollution to the environment is reduced.

Description

Technical field [0001] The invention provides a chitinase producing strain and a method for producing chitinase by fermentation of crab shells, belonging to the field of biotechnology. Background technique [0002] Chitinase is a class of hydrolases that can efficiently degrade β-1,4 glycosidic bonds in chitin. It is mainly found in animals, plants, fungi, bacteria, and actinomycetes. It is used in biological control, health care and resources of chitin. The field of chemical utilization has broad application prospects. Chitooligosaccharides are oligosaccharides obtained by enzymatic hydrolysis of chitin, and their applications in medicine, food and health care and plant defense have been explored, and have become a hot spot in chitin research. Studies have found that chitooligosaccharides with a degree of polymerization of 2-8 play an important role in lowering blood lipids and blood sugar, activating the body's immune system, anti-tumor and anti-infection. It can also extend ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12R1/01
CPCC12N9/2442C12Y302/01014C12N1/205C12R2001/01
Inventor 娄文勇徐培宗敏华程建华
Owner 广州增城潮徽生物技术有限公司
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