Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacterial strain capable of producing chitinase and application of bacterial strain to production of chitinase by fermenting crab shell

A chitinase and strain technology, applied in the directions of enzymes, enzymes, bacteria, etc., can solve the problems of restricting the large-scale use of chitinase, high production cost of enzyme preparations, and insufficient activity to meet the needs of industrialization, and achieve significant economic benefits and Environmental benefits, low production costs, and efficient hydrolysis

Active Publication Date: 2015-03-25
广州增城潮徽生物技术有限公司
View PDF2 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because chitinase has high economic development value and broad application prospects, there are many reports about chitinase-producing microorganisms, but there are still enzyme preparations with high production costs and insufficient activity to meet the needs of industrialization, which limits the development of chitinase. The Large-scale Application of Sulfase in Industry

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain capable of producing chitinase and application of bacterial strain to production of chitinase by fermenting crab shell
  • Bacterial strain capable of producing chitinase and application of bacterial strain to production of chitinase by fermenting crab shell
  • Bacterial strain capable of producing chitinase and application of bacterial strain to production of chitinase by fermenting crab shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for producing chitinase by fermentation of crab shells includes the following steps:

[0035] (1) Activation of Paenibacillus pasadenensis CS0611 Take the slant culture and place it in a 37°C constant temperature incubator for 30 minutes to activate the strain.

[0036] (2) Seed liquid culture

[0037] An appropriate amount of the slant culture was inoculated into the seed medium, and the seed medium was LB medium, which consisted of: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH 7.0. The culture conditions were 37°C, the rotating speed of the shaker was 180 rpm, and the culture time was 20 h.

[0038] (3) Liquid fermentation culture

[0039] Transfer the seed liquid to the fermentation medium with an inoculum of 4%. The fermentation medium consists of: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.08 g / L, potassium dihydrogen phosphate 0.02 g / L, and magnesium sulfate 0.04 g / L, sodium chloride 0.1g / L. The culture conditions were 30°C, the r...

Embodiment 2

[0044] The difference between this embodiment and Embodiment 1 is:

[0045] Liquid fermentation culture

[0046] The composition of the enzyme production medium is: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.07 g / L, potassium dihydrogen phosphate 0.03 g / L, magnesium sulfate 0.05 g / L, and sodium chloride 0.1 g / L. The inoculation amount is 6%, the culture condition is 30°C, the rotation speed of the shaker is 200 rpm, and the culture time is 48 hours.

[0047] After the fermentation is completed under the above conditions, the supernatant of the fermentation broth is centrifuged to obtain the crude chitinase enzyme solution, and the enzyme activity can reach 205.4mU / mL.

Embodiment 3

[0049] The difference between this embodiment and Embodiment 1 is:

[0050] Liquid fermentation culture

[0051] The composition of the enzyme production medium is: crab shell powder 10g / L, dipotassium hydrogen phosphate 0.08 g / L, potassium dihydrogen phosphate 0.02 g / L, magnesium sulfate 0.04 g / L, and sodium chloride 0.1 g / L. The inoculum amount is 5%, the culture condition is 30°C, the rotation speed of the shaker is 180 rpm, and the culture time is 72 hours.

[0052] After the fermentation is completed under the above conditions, the supernatant of the fermentation broth is centrifuged to obtain the crude chitinase enzyme solution, and the enzyme activity can reach 200.2mU / mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a bacterial strain capable of producing chitinase and application of the bacterial strain to production of chitinase by fermenting crab shell. The bacterial strain is paenibacillus pasadenensis CS0611 and is preserved in China Center for Type Culture Collection; the preservation number is CCTCC M2014458; the preservation date is October 8, 2014. The bacterium is a start strain; the crab shell is used as a carbon source and a nitrogen source and is fermented for producing chitinase. The fermentation technology is simple; the production cost is low; the enzyme activity of the fermentative supernatant liquid can reach 211.3mU / mL; the wastes of shrimp and crab shells can be efficiently hydrolyzed; the pollution to the environment is reduced.

Description

Technical field [0001] The invention provides a chitinase producing strain and a method for producing chitinase by fermentation of crab shells, belonging to the field of biotechnology. Background technique [0002] Chitinase is a class of hydrolases that can efficiently degrade β-1,4 glycosidic bonds in chitin. It is mainly found in animals, plants, fungi, bacteria, and actinomycetes. It is used in biological control, health care and resources of chitin. The field of chemical utilization has broad application prospects. Chitooligosaccharides are oligosaccharides obtained by enzymatic hydrolysis of chitin, and their applications in medicine, food and health care and plant defense have been explored, and have become a hot spot in chitin research. Studies have found that chitooligosaccharides with a degree of polymerization of 2-8 play an important role in lowering blood lipids and blood sugar, activating the body's immune system, anti-tumor and anti-infection. It can also extend ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N9/42C12R1/01
CPCC12N9/2442C12Y302/01014C12N1/205C12R2001/01
Inventor 娄文勇徐培宗敏华程建华
Owner 广州增城潮徽生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com