Plant electric signal based crop salt tolerance evaluation method

An evaluation method and salt tolerance technology, applied in the field of agricultural biology, can solve the problems of unsuitable evaluation of rare varieties, failure of early diagnosis, destructive measurement, etc., and achieve reliable evaluation effect, less seed consumption, and rapid evaluation

Inactive Publication Date: 2016-06-15
XIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a crop salt tolerance evaluation method based on plant electrical signals, which solves the problem that the existing crop salt tol

Method used

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  • Plant electric signal based crop salt tolerance evaluation method
  • Plant electric signal based crop salt tolerance evaluation method
  • Plant electric signal based crop salt tolerance evaluation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The maize variety Wanrui 168 with strong salt tolerance and Zhengdan 958 with weak salt tolerance were selected.

[0075] The plump seeds with consistent appearance were washed with distilled water, and the mass fraction was added with 0.2% HgCl 2 Disinfect and clean. Put the filter paper into the bottom of the petri dish, and put 10 seeds of the two varieties into the petri dish evenly. After adding an appropriate amount of distilled water to the culture dish, put it into an incubator for cultivation. After the seeds germinated, they were transplanted into hydroponic dishes for growth. Pour appropriate amount of distilled water every day to ensure its normal growth needs. When the crops germinate 3 to 4 leaves, the plant height is 10cm to 20cm.

[0076] A single crop seedling was placed in a Faraday cage, and test electrodes were placed on the leaves and roots respectively, and the surface potential signal of the leaves was collected with a biofunctional experiment...

Embodiment 2

[0080] The maize variety Wanrui 168 with strong salt tolerance and Zhengdan 958 with weak salt tolerance were selected.

[0081] The plump seeds with consistent appearance were washed with distilled water, and the mass fraction was added with 0.2% HgCl 2 Disinfect and clean. Put the filter paper into the bottom of the petri dish, and put 10 seeds of the two varieties into the petri dish evenly. After adding an appropriate amount of distilled water to the culture dish, put it into an incubator for cultivation. After the seeds germinated, they were transplanted into hydroponic dishes for growth. Pour appropriate amount of distilled water every day to ensure its normal growth needs. When the crops germinate 3 to 4 leaves, the plant height is 10cm to 20cm.

[0082] A single crop seedling was placed in a Faraday cage, and test electrodes were placed on the leaves and roots respectively, and the surface potential signal of the leaves was collected with a biofunctional experiment...

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Abstract

The invention discloses a plant electric signal based crop salt tolerance evaluation method. The method specifically comprises the steps of by acquiring surface potential signals of a germinated seed of a crop before and after salt stress, calculating characteristic values of the potential signals before and after the salt stress; and according to the changes of the characteristic values, defining a salt tolerance evaluation coefficient of the crop for evaluating the salt tolerance of the crop. According to the method, during crop salt tolerance evaluation, only a small amount of crop seedlings need to be subjected to the salt stress without a field experiment, the surface potential fluctuation signals of the seedlings are tracked and measured, and the characteristic values of the electric signals are calculated to evaluate the salt tolerance of the crop nondestructively and quickly. According to the method, the seed consumption is low and seeds do not need to be destroyed; and the method has the characteristic of nondestructive measurement. In addition, the method is used for tracking and measuring the electric signals of the seeds in the same batch, and the evaluation method is used for performing comparison according to the change degrees of the electric signal parameters of the crop before and after the salt stress, so that the individual difference of the seeds is avoided, culture and measurement methods are simple, and the evaluation effect is reliable.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology and relates to a method for evaluating crop salt tolerance based on plant electrical signals. Background technique [0002] With the rapid development of social economy, various natural and human factors make soil salinization more and more serious. 20% of the world's arable land has varying degrees of salinization, which is widely distributed in more than 100 countries and regions. The salinization of a large amount of soil poses a great threat to global food security and has become one of the most important ecological problems that seriously restrict agricultural production and human survival. In this situation, using various means to control and improve salinized soil, develop and cultivate salt-tolerant crops, improve the utilization rate of salinized soil, and maintain food production have become major issues for human beings today. [0003] In order to develop, utilize and cultiva...

Claims

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Application Information

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IPC IPC(8): G06F19/24G06Q50/02
CPCG06Q50/02G16B40/00
Inventor 刘锴习岗樊琳琳梁成贺瑞瑞
Owner XIAN UNIV OF TECH
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