Application of tomato TFT1 gene to improving salt tolerance of crops

A technology of salt tolerance and genetics, applied in the field of genetic engineering, can solve problems affecting tomato yield and quality, and achieve the effect of improving salt tolerance

Inactive Publication Date: 2011-10-19
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although tomato is highly adaptable to various climatic conditions, the stress of adverse environmental factors still has a great impact on yield. Salt stress is one of these adverse environments and seriously affects the yield and quality of tomato.

Method used

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  • Application of tomato TFT1 gene to improving salt tolerance of crops
  • Application of tomato TFT1 gene to improving salt tolerance of crops
  • Application of tomato TFT1 gene to improving salt tolerance of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Materials and Methods

[0013] Plant material, growth state, salt stress treatment

[0014] The way we cultivate tomatoes is hydroponics, and the nutrient solution is 1 / 5 Hoagland. We used MS plates (0.8% agar and 3% sucrose) to grow Arabidopsis. The treatments were 0, 80, 100, 115, 130 and 150 mM NaCl. We do germination test with about 100 Arabidopsis seeds, count them 5 days after sowing on MS plates, and the germination is defined as the radicle piercing the seed coat. For the growth test, we took the Arabidopsis thaliana with consistent growth after 5 days of normal germination as the material, moved it to the MS plate and grew it for 10 days, took the Arabidopsis plants (15 days old) and fixed them in liquid nitrogen, and stored them in - Refrigerate at 80°C for analysis. Each experiment was repeated three times, and 10 Arabidopsis plants were taken each time. In addition, each experiment was replicated at least twice (biological replicates).

[0015] Constru...

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PUM

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Abstract

The invention relates to the application of tomato TFT1 gene to improving the salt tolerance of crops, comprising the following steps of: obtaining the DNA sequence of TFT1, cloning the encoding area of TFT1 gene in a tomato body through the PCR method, connecting the encoding area to a modified vector pBI121 after sequencing identification (see figure 1), and transforming targeted crops through the floral dip method. The crops in which TFT1 is transplanted are all along endowed with higher APX activity no matter under a normal growth condition or salt stress condition, thereby indicating that the TFT1 has the function of improving the salt tolerance of crops.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to the application of tomato TFT1 gene in improving the salt tolerance of crops. Background technique [0002] Soil salinization is one of the important reasons for the reduction of grain production in my country. There are many ways and methods to improve saline land, and recent studies have shown that improving the genetic potential of plants to tolerate soil salinization is also one of the effective methods. In recent years, many studies have shown that plant 14-3-3 proteins may play a major role in regulating plant resistance to salt stress. People have done a lot of work on the mechanism of plant salt tolerance by using transgenic plants and mutants as materials, but there are few studies on the role of 14-3-3 protein in this aspect. Although 14-3-3 proteins may play a major role in regulating plant resistance to salt stress, direct evidence for this is scarce. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
Inventor 许卫锋施卫明
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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