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Screening method of growth-promoting bacterial strain for improving salt tolerance of crops

A screening method and strain technology, applied in the direction of bacteria, isolated microorganisms, fertilizer mixtures, etc., can solve the problems of poor effect, unstable effect, general strain function, etc., achieve outstanding application effect, improve colonization and functional expression ability Effect

Active Publication Date: 2017-05-31
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, according to the inventor's experience and related reports, most of the strains have general functions, poor or unstable effects, and the high-efficiency selection method of excellent strains / bacteria is very important.

Method used

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  • Screening method of growth-promoting bacterial strain for improving salt tolerance of crops
  • Screening method of growth-promoting bacterial strain for improving salt tolerance of crops
  • Screening method of growth-promoting bacterial strain for improving salt tolerance of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation of Plant Root Bacteria in Severe Saline-alkali Land and Detection of Its Growth-promoting Function

[0020] The plants growing on the heavily saline-alkali soil were collected in situ, put into a sterile sample bag, and the latitude and longitude, temperature and humidity of the samples were recorded, as well as the sampling habitat. Gently shake the plant sample to remove the loose soil attached to the roots, i.e. the rhizosphere soil. Weigh 1 g of rhizosphere soil, add 5 mL of normal saline, and vibrate with a vortex shaker for 1 min to obtain a soil suspension, which is rhizosphere soil sample 1. Cut off the root system, with a little stem attached to keep the roots all together. Put the root system in a PA bottle, add 5 mL of physiological saline, and vibrate at the maximum frequency on a vortex instrument for 1 min. The soil suspension is the rhizosphere soil sample 2. The roots were subsequently transferred to new PA bottles, surface sterili...

Embodiment 2

[0021] Example 2 Improving the excellent strain / line and strain combination screening method of saline-alkali land crop production performance

[0022] After activating the bacterial strains obtained by dilution and isolation, the bacterial cells were collected by centrifugation, washed twice with normal saline, and resuspended in 1 mL of normal saline for use. ①Prepare 1 / 3KMB plates containing 0% and 5% sodium chloride, pH=9.0, CAS, organic phosphorus, inorganic phosphorus, asbestos, and ADF 8 types of plates, and spot each bacterial suspension to 8 types in turn On the plate, cultured upside down at 30°C for 7 days. ② Inoculate the resuspension liquid into 1 / 3 KMB liquid medium containing tryptophan with a final concentration of 100mg / L according to the inoculum amount of 1%, and incubate at 30°C for 48-72h. IAA detection (500 μg / ml tryptophan) was then performed. Record the growth-promoting results of each strain of bacteria and organize them into an excel statistical tab...

Embodiment 3

[0024] Embodiment 3 full-featured strain combination and corn potted plant screening test

[0025] Based on the principle of functional complementarity, with the goal of covering as many biological functions as possible, the dominant strains with different functions were selected respectively, such as Bacillus amyloliquefaciens ZCM18 with strong functions of IAA production, phosphorus solubilization and nitrogen fixation [this strain was released in 2016 On September 12, it was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures (CGMCC for short, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code 100101), and the deposit number is CGMCCNo. 12959, classified as Bacillus amyloliquefaciens (Bacillus amyloliquefaciens)], a dominant strain of Bacillus amyloliquefaciens with strong siderophilic production, antagonism of pathogenic fungi and ethylen...

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Abstract

The invention provides a screening method of a growth-promoting bacterial strain for improving salt tolerance of crops. The screening method belongs to the technical field of microbe separation and application, and comprises the two steps of screening dominant strains and combining the dominant strains. The screening of the dominant strains is characterized by screening a series of salt-tolerance and growth-promoting functions so as to finally obtain the bacterial strains with multiple functions or superior advantages; the combining of the dominant strains is characterized by following the principle that the bacterial strains are in mutualism and functional complementation so as to provide the colonization and the function expression ability of bacterial strain combination in crop rhizospheres and realize an effect of 'one plus one is more than two'. The composite microbial system obtained through screening by the method provided by the invention has an outstanding field application effect, solves the problems that in similar research, the bacterial strain is single or adopts a simple combination method, so that the field application effect is instable and poor in effect, and is beneficial for greatly promoting the production of saline soil crops.

Description

technical field [0001] The invention relates to the technical field of microorganism isolation and application, in particular to a screening method for improving growth-promoting strains of crops with salt tolerance. Background technique [0002] At present, with the development of society and the increase of population, the demand for cultivated land resources is increasing. my country has 99.13 million hectares of saline-alkali land, including 700,000 hectares around the Bohai Sea, which is an important potential arable land resource. The improvement of saline-alkali land is an international problem. All countries in the world are trying to find scientific methods for improving saline-alkali land. At present, the available methods include physical improvement techniques, such as hydraulic salt method, seepage pipe salt drainage and some farming measures; chemical methods Improvement technology, such as the use of soil amendments; biological improvement technology, the use...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C05F15/00C05G3/04C05G3/80
CPCC05F9/04C05F11/08C12N1/02C12N1/20C05G3/80
Inventor 彭杰丽王占武胡栋张翠绵贾楠
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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