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Geobacillus kaustophilus NJRC-14 and microbial organic fertilizer thereof

A technology of NJRC-14 and Geobacillus, applied in the field of intensive agricultural production, can solve the problems of unstable control effect of microbial organic fertilizers in the field, improve the ability of colonization and function, simple production process, and fixed asset investment little effect

Active Publication Date: 2014-04-23
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The field control effect of this antagonistic bacteria and microbial organic fertilizer produced by rapeseed meal is unstable

Method used

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  • Geobacillus kaustophilus NJRC-14 and microbial organic fertilizer thereof
  • Geobacillus kaustophilus NJRC-14 and microbial organic fertilizer thereof
  • Geobacillus kaustophilus NJRC-14 and microbial organic fertilizer thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] (1) Isolation and identification of strains

[0029] 1. Screening of antagonistic bacteria that can use rapeseed meal to produce antagonistic substances

[0030] Collect the rhizosphere soil of healthy plants in bacterial wilt disease plots, uproot the healthy plants, and shake gently. After most of the soil shakes, the remaining soil close to the roots and some fibrous roots are rhizosphere soil. Take 1g of rhizosphere soil and place Place in a 50mL Erlenmeyer flask (containing glass beads) filled with 9mL sterile water, shake at 170rpm for 30min, serially dilute the soil suspension 10 times, take 100μL of the suspension and spread it on a rapeseed PDA medium plate, and incubate at 30°C for 2-4d . A single colony was picked and streaked on an RC medium plate (80g rapeseed meal, 20g agar, 1L distilled water, pH7.2, sterilized at 115°C for 30min) for purification.

[0031] The spot spraying method was used to detect the inhibitory ability of rhizosphere bacteria on the...

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Abstract

The invention belongs to the technique of agricultural intensive production, and discloses Geobacillus kaustophilus NJRC-14 and a microbial organic fertilizer thereof. The invention provides the Geobacillus kaustophilus strain NJRC-14, the Geobacillus kaustophilus strain NJRC-14 is capable of rapid proliferation at 30-50DEG C and produces a lot of soil-borne bacterial wilt pathogen growth inhibition substances by solid fermentation with rapeseed meal. The invention also provides a preparation method of the microbial organic fertilizer by use of the strain NJRC-14 and application, the microbial organic fertilizer is simple in production method, stable in quality and free of need of secondary fermentation, and has better economic and social benefits. Experiments show that, after the microbial organic fertilizer is applied into the soil, antagonistic bacteria can multiply rapidly to form a dominant group in the soil, the antagonistic bacteria uses the rapeseed meal to produce antagonistic substances to inhibit the growth of pathogens, and the biocontrol efficiency reaches to more than 75% in tomato bacterial wilt soil with serious morbidity.

Description

technical field [0001] The invention belongs to the agricultural intensive production technology, relates to a thermophilic Geobacillus NJRC-14 and its microbial organic fertilizer, which is specially used for overcoming and eliminating the problem of continuous cropping soil-borne bacterial wilt. Background technique [0002] Tomato bacterial wilt is a bacterial vascular disease caused by Ralstonia solanacearum (hereinafter referred to as Ralstonia solanacearum). The disease is a devastating soil-borne disease with a large variation of the pathogen and a wide range of hosts, including tomato, potato, eggplant, tobacco, etc., and it occurs all over the world. The disease is mainly distributed in the south of the Yangtze River in my country, and it is more common in Guangdong, Guangxi, Fujian, Zhejiang, Hunan, Jiangsu and other provinces. %, resulting in a failure of harvest and causing major economic losses to my country's agriculture. [0003] Commonly used strategies to c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01P1/00C12R1/01
Inventor 徐阳春韦中胡洁亚历山大·如赛
Owner NANJING AGRICULTURAL UNIVERSITY
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