Antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and microorganism organic fertilizer with antagonism actinomycetes
A technology for cucumber fusarium wilt and organic fertilizer, which is applied in the directions of organic fertilizer, preparation of organic fertilizer, treatment of biological organic parts, etc., can solve the problems of weakening the ability of sustainable agricultural development, loss of nutrient resources, waste of raw materials, etc. The ability to reproduce and function, the inhibitory effect is remarkable, the effect of high bioavailability
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[0032]1. Isolation and Identification of Strains
[0033] The plants and the surrounding soil that have suffered from continuous cropping obstacles and cucumber wilt symptoms were collected, and the pathogenic strain - Fusarium oxysporum cucumber-specific type was obtained by using Fusarium selective medium, and its pathogenicity was verified. Actinomycetes were isolated from the soil of Zijin Mountain in Nanjing, and antagonistic strains were screened by confrontation method using PDA medium. Then through the pot test, the high-efficiency antagonistic strains were re-screened, and the high-efficiency antagonistic strain CT205 was finally obtained and identified. The strains were stored in a refrigerator at 4°C.
[0034] Strain CT205 belongs to Streptomyces albospinus, and its main biological characteristics are: the best growth temperature on PDA medium is 28°C, cultured for 5-7 days, forming colonies with a diameter of 2-4mm, and the colonies are white in the early stage. T...
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