Antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and microorganism organic fertilizer with antagonism actinomycetes

A technology for cucumber fusarium wilt and organic fertilizer, which is applied in the directions of organic fertilizer, preparation of organic fertilizer, treatment of biological organic parts, etc., can solve the problems of weakening the ability of sustainable agricultural development, loss of nutrient resources, waste of raw materials, etc. The ability to reproduce and function, the inhibitory effect is remarkable, the effect of high bioavailability

Active Publication Date: 2013-09-04
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] On the other hand, the incineration of rice, wheat, corn, rapeseed and other crop straws, and the disposal of livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste the organic fertilizer and bio-organic fertilizer products. Raw materials; a large number of nutrient resources (C, N, P, K, S and trace elements) are lost outside the soil-plant system, which obviously weakens the ability of my country's sustainable agricultural development

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  • Antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and microorganism organic fertilizer with antagonism actinomycetes
  • Antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and microorganism organic fertilizer with antagonism actinomycetes
  • Antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and microorganism organic fertilizer with antagonism actinomycetes

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Embodiment Construction

[0032]1. Isolation and Identification of Strains

[0033] The plants and the surrounding soil that have suffered from continuous cropping obstacles and cucumber wilt symptoms were collected, and the pathogenic strain - Fusarium oxysporum cucumber-specific type was obtained by using Fusarium selective medium, and its pathogenicity was verified. Actinomycetes were isolated from the soil of Zijin Mountain in Nanjing, and antagonistic strains were screened by confrontation method using PDA medium. Then through the pot test, the high-efficiency antagonistic strains were re-screened, and the high-efficiency antagonistic strain CT205 was finally obtained and identified. The strains were stored in a refrigerator at 4°C.

[0034] Strain CT205 belongs to Streptomyces albospinus, and its main biological characteristics are: the best growth temperature on PDA medium is 28°C, cultured for 5-7 days, forming colonies with a diameter of 2-4mm, and the colonies are white in the early stage. T...

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Abstract

The invention discloses antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and a microorganism organic fertilizer with the antagonism actinomycetes, belonging to intensive agriculture production technology. The antagonism actinomycetes CT205, which has an obvious antagonism effect on pathogenic fungus of cucumber fusarium wilt and is obtained by separation, belongs to streptomyces albospinus, and the culture preservation number of the antagonism actinomycetes is CGMCC (China General Microbiological Culture Collection Center) NO.7434. Antagonistic bacteria liquid is fermented and then is subjected to solid fermentation together with organic materials to prepare the microorganism organic fertilizer. Experiments show that after the microorganism organic fertilizer is applied into soil, the antagonistic bacteria can breed rapidly and form a dominant bacterial community in the soil, and the preventive rate of the antagonism actinomucetes in soil with serious cucumber fusarium wilt can reach more than 80%.

Description

technical field [0001] The invention relates to an antagonistic actinomycete for preventing and eliminating continuous-cropping cucumber wilt and microbial organic fertilizer thereof, which belongs to the technical field of agricultural intensive production and is specially used for overcoming and eliminating the problem of continuous-cropping cucumber wilt. Background technique [0002] Cucumber is an important economic crop in my country. Cucumber wilt is one of the main diseases of cucumber, which occurs all over the country, especially in protected planting. Cucumber wilt is a soil-borne disease caused by Fusarium oxysporum. It is widely distributed in tropical, subtropical and some warm regions all over the world. It was first discovered and reported in the Netherlands in 1943. At present, in my country's highly intensive cucumber planting areas, when Fusarium wilt occurs, the yield loss is more than 80%. Spread and have a strong ability to adapt to the environment. Fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C05F11/08C05F17/00C12R1/465
CPCY02W30/40
Inventor 沈其荣王世梅梁银张瑞福
Owner NANJING AGRICULTURAL UNIVERSITY
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