Salt tolerance screening and identification method for corn in seedling stage

Abstract

The invention provides a salt tolerance screening and identification method for corn in the seedling stage. It is found and verified that salt tolerance and zinc sensitivity of a corn selfing line are consistent. Whether a corn variety is tolerant to salt is judged primarily according to whether corn seedlings subjected to zinc deficiency stress treatment have zinc deficiency symptoms or not, further, zinc stress treatment is conducted on added zinc subjected to zinc deficiency stress treatment to further judge whether the corresponding corn variety is tolerant to salt, the two steps are combined, and screening and identification precision is improved. Besides, the method is practical and effective, operation is easy to implement, chemicals are not needed for detection, and accordingly cost is low. The method is free of toxin and harm to human bodies and free of environment pollution, and lays a certain theoretical foundation for large-scale screening of corn salt-tolerant selfing lines and breeding of salt-tolerant crops.

Description

technical field [0001] The invention relates to the technical field of crop planting, in particular to a method for screening and identifying the salt tolerance of seedling corn. Background technique [0002] Soil salinization is one of the main factors causing crop yield reduction. According to statistics, about 20% of the agricultural land in the world is getting more and more salinized. Our country has 2.6×10 7 hm 2 of saline land, which has 6.6 x 10 6 hm 2 It is the area of ​​cultivated land, which is mainly concentrated in the main grain-producing areas such as North China, Northeast China, the inland areas of Northwest China and the north of the Yangtze River. In recent years, due to adverse factors such as environmental impact and human activities, more and more cultivated land is facing the danger of secondary salinization. It is estimated that by 2050, more than 50% of the cultivated land in the world will be salinized. Therefore, the comprehensive utilizatio...

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Problems solved by technology

[0016] The above-mentioned determination of the content of each substance is costly, the dosage is large, and the operation steps are cumbersome, and there is a certain risk (if the operation is not done properly during the required heating process, the liquid is easy to spray out and burn the skin)

Some chemicals required for the determination of malondialdehyde, hydrogen peroxide, soluble sugar, proline, etc. include thiobarbituric acid (TBA), ninhydrin (Ninhydrin), toluene, anthrone (Anthrone) The cost is high, and it is highly toxic, it is harmful to the human body, it is not easy to decompose, and it pollutes the environment

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