Plant salt-tolerance-related protein AtSDIP1, and coding gene and application thereof

A related protein and plant technology, applied in the plant salt tolerance related protein AtSDIP1 and its coding gene and application field, can solve the problems of plant root water absorption difficulty, membrane lipid nucleic acid damage, ion poisoning, etc.

Inactive Publication Date: 2013-04-24
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The impact of salt stress on plant growth is mainly manifested in the following aspects: first, excessive salinity in the soil reduces the osmotic potential of the soil, which will cause difficulty in absorbing water for plant roots, resulting in water shortage for plants; second, sodium ions, Ion poisoning caused by the accumulation of ...

Method used

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  • Plant salt-tolerance-related protein AtSDIP1, and coding gene and application thereof
  • Plant salt-tolerance-related protein AtSDIP1, and coding gene and application thereof
  • Plant salt-tolerance-related protein AtSDIP1, and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Embodiment 1, the acquisition of AtSDIP1 gene overexpression plant and AtSDIP1 gene suppression expression plant

[0102] 1. Construction of overexpression vector

[0103] Replace the small fragment between the SmaI and XbaI restriction sites of the vector pCAMBIA1300-221 with the double-stranded DNA molecule shown in the 85th-747th nucleotide of the sequence 2 from the 5' end to obtain the recombinant plasmid pCAMBIA1300-221 - AtSDIP1 (i.e. overexpression vector). The schematic diagram of the structure of the recombinant plasmid pCAMBIA1300-221-AtSDIP1 is shown in figure 1 , AtSDIP1 gene is regulated by 35S double promoter.

[0104] 2. Construction of Inhibition Expression Vector

[0105] The double-stranded DNA molecule shown in sequence 3 of the sequence listing (in sequence 3, nucleotides 1 to 663 from the 5' end is the reverse complementary fragment of the AtSDIP1 gene, and nucleotides 664 to 853 are spacer fragments , the 854th to 1516th nucleotides are the At...

Embodiment 2

[0125] Example 2, Salt tolerance identification of AtSDIP1 gene overexpression plants and AtSDIP1 gene suppression expression plants

[0126] The T of two AtSDIP1 gene overexpression lines (SDIP1-OX#1 strain and SDIP1-OX#2 strain) were randomly selected. 3 For generation seeds, T 3 Substitute seeds, take the seeds of Columbia ecotype Arabidopsis (WT) plants, and take the T of empty vector plants 3 Subsequent salt-tolerance identifications were carried out on the generation seeds:

[0127] 1. Surface sterilize the seeds with 10% bleach, and then wash 3 times with sterile water.

[0128] 2. Group processing

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Abstract

The invention discloses a plant salt-tolerance-related protein AtSDIP1, and a coding gene and an application thereof. The protein provided by the invention is the following (a), or (b), or (c): (a) a protein composed of an amino acid sequence represented by the sequence 1 in the sequence table; (b) a protein derived from the sequence 1 and is related to plant salt tolerance, wherein the amino acid sequence of the sequence is subjected to substitution and/or deletion and/or addition of one or more amino acid residues; (c) a protein derived from the sequence 1 and is relates to plant salt tolerance, wherein the protein has at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 90%, at least 95%, at least 98%, or at least 99% homology with the sequence 1. The invention provides a basis for the researches of plant response and tolerability molecular mechanisms against salt stress, and has important significance to crop improvement and salt-tolerant crop cultivation.

Description

technical field [0001] The invention relates to a plant salt tolerance related protein AtSDIP1 and its coding gene and application. Background technique [0002] According to incomplete statistics, at least 20% of the cultivated land and more than 50% of the irrigated land in the world are affected by salt damage to varying degrees. In some arid or semi-arid areas, due to large evaporation and low precipitation, the salt in the soil (mainly NaCl and NaCO 3 ) accumulate in large quantities. In some coastal areas, the groundwater level is high or the seawater intrudes, which will also lead to the accumulation of more salt on the soil surface (mainly NaCl and MgSO 4 ). Unreasonable irrigation methods and long-term freshwater salt washing have caused secondary salinization of a large number of farmlands. With the rapid increase of the world's population, the intensification of urbanization in developing countries, and the sharp decline in the area of ​​irrigable land caused ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/21C12N15/82C12N15/84A01H5/00A01H1/02
Inventor 谢旗张华伟崔凤
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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