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Vertical compact panicle gene and application thereof

An amino acid and nucleotide sequence technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of no report of erect panicle gene cloning

Active Publication Date: 2009-12-09
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the cloning of the erect panicle gene has not been reported

Method used

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  • Vertical compact panicle gene and application thereof
  • Vertical compact panicle gene and application thereof
  • Vertical compact panicle gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0370] Example 1: Comparative study of rice varieties 313 and 314

[0371] This study mainly used a pair of near-isogenic lines, 313 (erect dense panicle, figure 1 right) and 314 (curved and loose spikes, figure 1 left).

[0372] 314 is a curved loose-ear variety with Wuyun japonica background discovered in the field of Shaoxing Academy of Agricultural Sciences in Zhejiang. The predecessor of 313 was the erect dense panicle variety with Wuyun japonica background discovered in the field of Zhejiang Shaoxing Academy of Agricultural Sciences. Later, 314 was used as the recurrent parent, and 313 was bred through multiple generations of backcrossing. Currently, 8 generations have been backcrossed. 314 did not contain the Depl gene, because the corresponding segment of 314 was sequenced, and its sequence was consistent with that of Nipponbare (bent and loose ears) (see SEQ ID NO: 2 and 3). The Dep1 gene is unique to 313.

[0373] The seed samples of 313 and 314 have been preserv...

Embodiment 2

[0397] Example 2: Cloning and sequencing of rice Dep1 gene, acquisition of genome sequence, isolation of promoter and 3'UTR region

[0398] We first cloned the erect panicle gene Dep1 (dense and erect panicle) of the Balilla type by using the F2 segregation population and using map-based cloning. We isolated the promoter region of this gene. Specific method: In order to clone the Dep1 gene, multiple populations were constructed successively. First, Shennong 265, a high-yield erect and dense panicle variety in Northeast China, and Qianchonglang, respectively the same as the japonica rice varieties Nipponbare and Zhonghua 11, were used to locate a main effector responsible for erect and dense panicle. QTL (quantitative trait loci), located on the long arm of chromosome 9, between SSR markers RM3700 and RM7424. In order to fine-tune the Dep1 gene, a larger F2 population was constructed: a japonica rice variety W101 containing the Dep1 gene was crossed with NJ6, and another japon...

Embodiment 3

[0406] Example 3: Gene Transformation

[0407] The function of the gene Dep1 was proved by the study of genetic complementation and overexpression of this gene.

[0408] (1) Experimental method

[0409] ◆Construction of complementary carrier:

[0410] The promoter and 3'UTR region of the Dep1 gene were isolated, then the ORF of Dep1 was connected in the middle, and finally connected to the vector pCAMBI1300 to construct: pDep::Dep1. Transform into Agrobacterium GV3101, and then into 314 through the method mediated by Agrobacterium. The specific construction process is as follows:

[0411] Firstly, primers 5'- ctgcag tcgtaacccatgctgtctca-3' and 5'- aagctt tggcgagtaaatgagtccaa-3', using 313 (NIL-Dep1, a near-isogenic line containing Dep1 gene) genomic DNA as a template to amplify the 900bp 3'UTR region of the Dep1 gene, connected to the vector pBluescript (stratagene), and sequenced correctly Digested with Pst I and HindIII, the fragment was connected to the binary vecto...

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Abstract

The invention relates to a vertical compact panicle gene and the application thereof, in particular to separated polynucleotide which comprises any one shown nucleotide sequence in SEQ ID NO: 1 and 5 to 8 or a nucleotide sequence which is basically the same with any one shown nucleotide sequence in the SEQ ID NO: 1 and 5 to 8. The invention also relates to a carrier containing the gene, a host cell, a plant and a method for producing the plant. The gene can be used for enhancing the yield of crops and plants or improving the crops and the plants.

Description

technical field [0001] The present invention relates to erect panicle gene and its application. The invention also relates to vectors, host cells and plants comprising the gene and methods for producing plants. The gene can be used to increase crop plant yield or improve crop plant traits. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population uses rice as the main food. In the current situation of rapid population growth and decreasing arable land, how to effectively increase rice production has become a very important task in agricultural production. [0003] In the 1960s, the dwarf gene "sd1" was used for dwarf breeding. This gene encodes a key enzyme in the synthesis of gibberellin, which led to the first breakthrough in rice yield, known as the first "green revolution" in history. [1] . In fact, this revolution is a revolution in plant type, that is, dwarf breeding, which has profoundly a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H1/00A01H4/00A01H5/12C07K14/415C12N15/11
CPCC12N15/8269C07K14/415C12N15/8261Y02A40/146
Inventor 傅向东黄先忠钱前刘正斌
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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