Promoters for regulating gene expression in plants

A promoter and plant technology, which can be applied in the fields of plant genetic improvement, plant products, genetic engineering, etc., and can solve problems such as the adverse effects of non-pest insects and the adverse effects of transgenic plants on male fertility

Pending Publication Date: 2022-04-08
SYNGENTA PARTICIATIONS AG
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Because plant pollen may be a food source for some non-pest insect species, or it is hypothesized that plant pollen may be carried by wind and deposited on non-pest insect host plants, regulators regulating the commercialization of GM crops are concerned about certain insecticidal properties of pollen. High levels of expression of proteins (e.g., certain Cry proteins) may adversely affect localized populations of non-pest insects
In addition, expression of certain insecticidal proteins in pollen has been observed to adversely affect male fertility in transgenic plants

Method used

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  • Promoters for regulating gene expression in plants
  • Promoters for regulating gene expression in plants
  • Promoters for regulating gene expression in plants

Examples

Experimental program
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example

[0174] The present invention may be further described by reference to the following specific examples. These examples are provided for illustrative purposes only and are not intended to be limiting unless otherwise stated. Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and described in: Ausubel (editor), Current Protocols in Molecular Biology, John Wiley and Sons, Inc. [John Wiley & Sons] (1994); J. Sambrook et al., Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press [冷泉港, New York: Cold Spring Harbor Laboratory Press] (2001); and T.J. Silhavy, M.L. Berman and L.W. Enquist, Experiments with Gene Fusions, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY [Cold Spring Harbor Laboratory Press] , Cold Spring Harbor, New York (1984).

example 1

[0175] Example 1. Identification of maize regulatory elements.

[0176] Regulatory elements from maize genes were identified on Syngenta's proprietary (Zm80K) Affymetrix array, which were highly expressed in multiple tissue types but not expressed in pollen, i.e. very low or no expression in pollen detected. Regulatory elements from 8 different maize genes were used to generate constructs to drive the expression of insect control genes in transgenic maize tissues and plants. A description of the regulatory elements used is shown in Table 1.

[0177] Table 1. Description of the regulatory elements used to prepare the expression constructs.

[0178]

[0179]

[0180] A terminator element was also identified from the same gene from which the promoters identified above were cloned. See Table 2 for terminator descriptions.

[0181] Table 2. Description of terminator elements of the invention.

[0182]

[0183]

example 2

[0184] Example 2: Construction of non-pollen expression vector

[0185] To test the above-mentioned regulatory elements (promoter and terminator) in transgenic maize plants, 8 binary vectors were prepared. Each binary vector contains two expression cassettes.

[0186] The first expression cassette comprises an eFMV / e35S transcriptional enhancer operably linked to the promoter of the invention to be tested, which is linked to a heterologous coding sequence (cry1Ab) encoding the Cry1Ab insecticidal protein (cry1Ab) (US Pat. No. 5,625,136), the heterologous coding sequence is operably linked to the matching terminator sequence of the promoter. For example, an expression cassette prepared to test the prZm061393-01 promoter (SEQ ID NO: 2) contained the tZm061393-01 terminator (SEQ ID NO: 13).

[0187] The second expression cassette, which is the same for each binary vector, contains the maize ubiquitin promoter (prUbi1-10) (Christensen et al., 1992 PMB 18:675), which is associate...

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Abstract

The present invention relates to promoters having particular utility in driving root-specific expression of heterologous genes conferring enhanced agronomic, horticultural and / or pesticidal properties to a given transgenic plant. The invention also relates to a DNA molecule comprising the promoter of the invention and a transformed plant tissue and a seed thereof comprising a DNA molecule comprising the promoter of the invention operably linked to one or more heterologous genes.

Description

[0001] sequence listing [0002] The Sequence Listing in ASCII text format, filed pursuant to 37 C.F.R. §1.821, entitled "81937-REG-ORG-NAT-1_ST25.txt", 239 kilobytes in size, is provided as an alternative to a paper copy, at Generated and submitted via EFS-Web on September 4, 2019. This Sequence Listing is hereby incorporated by reference into this specification for its disclosure. technical field [0003] The present invention relates generally to the field of plant molecular biology and regulation of gene expression in plants. Transgenic eukaryotes (including transgenic plant cells, plants and seeds) whose genomes include molecular constructs for controlling expression of heterologous genes are also disclosed. Background technique [0004] GM crops consist of increasingly complex genetic modifications, including multiple transgenes that confer different traits, also known as "gene stacking" or "trait stacking." For example, many transgenic corn products currently on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C12N15/32C12N15/74C12N15/82
CPCC12N15/8225C12N15/8231C12N15/8286Y02A40/146C12N15/8205C12N15/8223
Inventor K·阿扎卡纳达姆周爱灵J·康维尔J·D·克拉克五世
Owner SYNGENTA PARTICIATIONS AG
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