Method for evaluating crop salt tolerance by utilizing external light to induce seed to illuminate

A technology of external light and salt tolerance, applied in the field of agricultural biology, can solve the problems of unsuitable evaluation of rare varieties, failure of early diagnosis, destructive measurement, etc., and achieve the effect of reliable evaluation, less seed consumption, and rapid evaluation

Inactive Publication Date: 2015-06-03
XIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for evaluating the salt tolerance of crops by using external light to induce seed luminescence, which solves the problem that the existing crop salt tolerance evaluation method has a large amount of samples, a long cycle, cannot be diagnosed early, and is not suitable for destructive measurement. Problems with Rare Species Evaluation

Method used

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  • Method for evaluating crop salt tolerance by utilizing external light to induce seed to illuminate
  • Method for evaluating crop salt tolerance by utilizing external light to induce seed to illuminate
  • Method for evaluating crop salt tolerance by utilizing external light to induce seed to illuminate

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Experimental program
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Effect test

Embodiment

[0054] Wanrui 168 with strong salt tolerance and Zhengdan 958 with weak salt tolerance were selected.

[0055] The plump seeds with consistent appearance were washed with distilled water, and the mass fraction was added with 0.2% HgCl 2 After disinfection and cleaning, dry the surface moisture with filter paper, place 7 seeds of each of the two varieties in the sample cup of the sample room of the BPCL weak luminescence measuring instrument (developed by the Institute of Biophysics, Chinese Academy of Sciences), and measure after dark adaptation for 10 minutes Spontaneous luminescence intensity of seeds I SL ,, the measurement time is 60s, the time interval of collecting data is set to 1s, and the working voltage is -1000V. Before each measurement, the background was determined and subtracted. I SL The unit is counts / s.

[0056] After measuring the spontaneous luminous intensity I SL Finally, the same batch of seeds was irradiated with blue LED for 30s, and the luminescen...

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Abstract

The invention discloses a method for evaluating crop salt tolerance by utilizing external light to induce a seed to illuminate. The method specifically comprises the following steps of respectively measuring spontaneous luminescence intensities of an ungerminated seed and a salt-stressed germinated seed, respectively measuring luminescence intensity attenuation curves of the ungerminated seed and the salt-stressed germinated seed under the irradiation of the external light, and obtaining delay luminescence kinetic parameters of the ungerminated seed and the germinated seed; evaluating the salt tolerance of the seeds according to the variation of the kinetic parameters of the luminescence intensity attenuation curves. According to the method for evaluating the crop salt tolerance by utilizing the external light to induce the seed to illuminate, when a crop salt tolerance evaluation is performed, field trials are unnecessary, only a few germinated seeds are salt-stressed necessarily, the luminescence attenuation curve of the germinated seed, which is induced by the external light, is measured in a track manner, the salt tolerance of the seed is losslessly and quickly evaluated by means of extracting and analyzing the kinetic parameters of the luminescence attenuation curves, and the features of less seed usage and nondestructive measurement are obtained. In addition, the method for evaluating the crop salt tolerance by utilizing the external light to induce the seed to illuminate is used for tracking and measuring the same batch of seeds, and evaluating the seeds according to the variation degree of the seed spontaneous luminescence parameters, so that the evaluation result is more reliable.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology and relates to a method for evaluating the salt tolerance of crops by using external light to induce seed luminescence. Background technique [0002] With the rapid development of social economy, various natural and human factors make soil salinization more and more serious. 20% of the world's arable land has varying degrees of salinization, which is widely distributed in more than 100 countries and regions. The salinization of a large amount of soil poses a great threat to global food security and has become one of the most important ecological problems that seriously restrict agricultural production and human survival. In this situation, using various means to control and improve salinized soil, develop and cultivate salt-tolerant crops, improve the utilization rate of salinized soil, and maintain food production have become major issues for human beings today. [0003] In order t...

Claims

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Application Information

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IPC IPC(8): A01G7/00
CPCA01C1/00A01G7/00G01N17/00
Inventor 习岗刘锴赵燕燕贺瑞瑞
Owner XIAN UNIV OF TECH
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