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Rhizosphere growth-promoting bacterium for enhancing salt tolerance of crops and microbial fertilizer and application of bacterium

A technology of microbial fertilizer and ability, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of difficult land use and achieve the effect of improving soil, low cost, and low raw material cost

Active Publication Date: 2019-08-13
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to aim at the practical problem that the crop yield in saline-alkali land is low and the land is difficult to use in agricultural production, develop and develop microbial organic fertilizers that can improve the salt stress tolerance of saline-alkali crops, increase the yield of crops on saline-alkali land and improve the soil of saline-alkali land to a certain extent Physical and chemical properties

Method used

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  • Rhizosphere growth-promoting bacterium for enhancing salt tolerance of crops and microbial fertilizer and application of bacterium
  • Rhizosphere growth-promoting bacterium for enhancing salt tolerance of crops and microbial fertilizer and application of bacterium
  • Rhizosphere growth-promoting bacterium for enhancing salt tolerance of crops and microbial fertilizer and application of bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, sample collection and strain screening

[0063] 1. Sample collection and strain isolation

[0064] The saline-alkali soil from all over the country was collected and potted experiments were carried out. The corn variety Jingtian Zihuanuo was planted on these soils, watered normally, and the corn plants were harvested one month later. The soil suspension of the rhizosphere soil was extracted separately, and the soil suspension was diluted and spread on LB solid medium after bathing at 85°C for 15 minutes, and cultured at 37°C overnight. The strains on the plate were separated and purified, and a total of Bacillus 124 was screened. strain.

[0065] 2. Primary screening of strains

[0066] Strains to be tested: 124 bacillus strains screened in step one.

[0067] 1. Disinfect the surface of the seeds of the corn variety Jingtian Zihuanuo, put them in a sterilized petri dish (place a sterilized filter paper moistened with sterile water at the bottom of the p...

Embodiment 2

[0106] Example 2, Determination of Contents of Substances Related to Adversity Stress in Maize Plants Inoculated with Bacillus T1-8

[0107] 1. Disinfect the surface of the seeds of the corn variety Jingtian Zihuanuo, put them in a sterilized petri dish (place a sterilized filter paper moistened with sterile water at the bottom of the petri dish), and vernalize at 4°C for 3 days in the dark.

[0108] 2. After completing step 1, put the germinated seeds in a round plastic basket, place 30 seeds on each small basket, put the plastic basket on the 1.6L small bucket, add about 1.4L of water to contact with the seeds, and use black to avoid The light film is shaded, cultured at 25°C for 4 days, and the water is changed once a day.

[0109] 3. After completing step 2, remove the black light-shielding film, place at 25°C for one week, and change the water once a day in the morning and evening.

[0110] 4. After completing step 3, replace the water in the small bucket with 1 / 4MS nutr...

Embodiment 3

[0133] Embodiment 3, determination of other growth-promoting ability of Bacillus sp. T1-8

[0134] 1. NH 3 production capacity assay

[0135] Insert Bacillus T1-8 into a test tube filled with peptone water culture solution, 28°C, 170rmin -1 Shake culture for 72h, add 1mL Nessler's reagent (0.09mol / L potassium mercuric iodide and 2.5molL -1 Potassium hydroxide mixture), the strains with yellow-brown precipitates are positive for ammonia production.

[0136] Peptone water culture medium: peptone powder 10g, NaCl 5g, distilled water to 1000mL, pH7.8.

[0137] 2. Determination of siderophilic production capacity

[0138] Spot Bacillus T1-8 on the LB solid medium plate, culture at 28°C for 24 hours, then pour the CAS solid medium and spread it on the LB plate. After 15-30 minutes, observe whether there is light yellow or light red around the bacteria The halos were used to preliminarily determine its siderophilic ability.

[0139] Inoculate Bacillus T1-8 in LB liquid medium, ...

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Abstract

The invention discloses a rhizosphere growth-promoting bacterium for enhancing the salt tolerance of crops and microbial fertilizer and application of the bacterium. The preservation number of provided bacillus paralicheniformis T1-8 is CGMCC No. 17335. The bacillus paralicheniformis T1-8 is inoculated to the rhizospheres of potted corn and wheat, and the growth indexes of the corn and wheat can be increased under salt stress. The microbial organic fertilizer is prepared by means of secondary fermentation after a bacterial liquid fermented by the bacterium is uniformly mixed with common cow manure, common pig manure and straw for composting, and can increase the yield of corn on saline-alkali land and improve the physical and chemical properties of soil to a certain extent. The bacterium has important significance for the development of functional microbial organic fertilizer capable of enhancing the salt tolerance stress of crops and increasing the yield of the crops on saline-alkaliland.

Description

technical field [0001] The invention relates to the technical field of production of agricultural biological products, in particular to a rhizosphere growth-promoting bacterium that enhances the salt-tolerant ability of crops, as well as its microbial fertilizer and its application. Background technique [0002] At present, a large amount of arable land in the world is affected by salt damage. Excessive salinity in the soil will inhibit the growth of crops and reduce crop yields. Soil salinization is an important problem facing global agricultural development. Irrigation water and seawater containing trace amounts of sodium chloride It is the most important reason for increasing the salt content in farmland soil. The increase of NaCl content in soil will reduce the ability of plants to absorb water, and salt stress will affect seed germination through osmotic stress, ion poisoning, oxidative damage, and inhibition of enzyme activity. It is known that there are many ways to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/07
CPCC12P1/04C12N1/205C12R2001/07
Inventor 张瑞福陈小娟李盼刘云鹏徐志辉邵佳慧
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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