A method for screening and identifying salt tolerance of seedling corn

Abstract

The invention discloses a screening and identification method of salt tolerance of corns at a seedling stage. According to the screening and identification method disclosed by the invention, the situation that the salt tolerance of the corns is consistent with phosphorus sensibility and zinc sensibility of the corns is discovered and verified; whether a corresponding corn variety is salt-tolerant can be indirectly judged by judging whether the corns at the seedling stage have a phosphorus deficiency symptom under phosphorus deficiency stress treatment; then, zinc stress treatment is carried out, corn seedlings growing from a good state to a bad state before and after the zinc stress treatment are not a salt-tolerant corn variety, and corn seedlings growing from a bad state to a good state before and after the zinc stress treatment are a salt-tolerant corn variety. According to the screening and identification method disclosed by the invention, the successive two steps are combined, thereby improving the accuracy of screening and identification; the method is practicable and effective, is simple and convenient to operate and does not need chemical medicines for detection, therefore, the cost is low, and the method has no toxicity to human bodies and no pollution to the environment and lays the certain theoretical foundation for further screening corn salt-tolerant inbred lines in a large volume and cultivating salt-tolerant crops.

Description

technical field [0001] The invention relates to the technical field of crop planting, in particular to a method for screening and identifying the salt tolerance of seedling corn. Background technique [0002] Soil salinization is one of the main factors causing crop yield reduction. According to statistics, about 20% of the agricultural land in the world is getting more and more salinized. Our country has 2.6×10 7 hm 2 of saline land, which has 6.6 x 10 6 hm 2 It is the area of ​​cultivated land, which is mainly concentrated in the main grain-producing areas such as North China, Northeast China, the inland areas of Northwest China and the north of the Yangtze River. In recent years, due to adverse factors such as environmental impact and human activities, more and more cultivated land is facing the danger of secondary salinization. It is estimated that by 2050, more than 50% of the cultivated land in the world will be salinized. Therefore, the comprehensive utilizatio...

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Problems solved by technology

[0016] The above-mentioned determination of the content of each substance is costly, the dosage is large, and the operation steps are cumbersome, and there is a certain risk (if the operation is not done properly during the required heating process, the liquid is easy to spray out and burn the skin)

Some chemicals required for the determination of malondialdehyde, hydrogen peroxide, soluble sugar, proline, etc. include thiobarbituric acid (TBA), ninhydrin (Ninhydrin), toluene, anthrone (Anthrone) The cost is high, and it is highly toxic, it is harmful to the human body, it is not easy to decompose, and it pollutes the environment

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