Industrialized seedling raising method for tissue culture seedlings of Eucalyptus robusta in autumn and winter in Chongqing region

Abstract

The invention discloses an industrialized seedling raising method for tissue culture seedlings of Eucalyptus robusta in autumn and winter in the Xiaoqing region, and relates to the technical field of plant seedling raising. The seedling culture method comprises the following steps: transplanting tissue culture seedlings of Eucalyptus robusta after seedling hardening into a completely infiltrated matrix section for culture, spraying a winter seedling culture compound foliar fertilizer during culture, and then putting container seedlings into a tray and putting the tray into a Eucalyptus robusta winter seedling culture shed for culture. Wherein the Eucalyptus robusta winter seedling raising shed sequentially comprises an elevated seedling raising system, a second-stage heat preservation shed and a third-stage heat preservation shed from inside to outside, a two-layer air cushion buffer structure can keep the temperature in the shed stable, the elevated seedling raising system comprises a seedbed, a water retention and root promotion layer and an inner heat preservation shed, and the water retention and root promotion layer is made of acrylic cotton. The invention also provides a formula of the compound foliar fertilizer for seedling raising in winter. The seedling raising method provided by the invention effectively solves the problems of excessive growth of stems of Eucalyptus robusta seedlings in winter, hibernation of tissue culture seedlings of Eucalyptus robusta and easy damping-off due to high humidity.

Description

Technical Field

[0001] The invention relates to the technical field of plant seedling cultivation, and in particular to a factory-based seedling cultivation method for Eucalyptus luxurianthus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area. Background Art

[0002] Eucalyptus cloeziana is a tall tree species of the Eucalyptus subgenus of the genus Eucalyptus. It is naturally distributed in Queensland, Australia (25-950m above sea level), and has been introduced and cultivated in southern provinces and autonomous regions such as Sichuan, Guangxi, Guangdong, Fujian, Hainan and Yunnan in my country. Eucalyptus cloeziana grows rapidly, with obvious apical dominance, a straight and round trunk, and can reach a height of 35-40m; the trunk is straight and round, with a high yield rate; the wood has a high basic density and excellent quality, making it a medium- and large-diameter solid wood species with great cultivation value.

[0003] Like other forest seedlings, Eucalyptus regia seeds under natural conditions are dormant in autumn and winter and will not germinate, so as to ensure that the seedlings will not be frozen to death. For the clones cultivated by tissue culture, the early proliferation and rooting stages are completed in the culture room with stable light and temperature, and only the later stages of hardening and transplanting are carried out outdoors. Therefore, the early stage of Eucalyptus regia tissue culture seedlings can theoretically be carried out throughout the year, while the later stage is in late autumn to early spring when the light and temperature are not suitable, and it is easy to have poor growth, which is specifically manifested in stagnant root growth, thin and slender stem segments, yellowing leaves, frequent damping-off diseases, etc., which have a great impact on the quality of seedlings.

[0004] Eucalyptus plants are naturally distributed along the coast of Australia, where the climate types are mainly tropical rainforest climate and subtropical humid climate, and the temperature is much higher than that of provinces in the Yangtze River Basin in my country. Eucalyptus plants can be grown in winter in areas with higher winter temperatures, such as Hainan, my country, Brazil, etc. If seedlings are grown in autumn and winter in areas with distinct four seasons, methods such as insulation, heating, and supplementary lighting can be used to meet the needs of seedling growth.

[0005] Sichuan is the northernmost province where artificial introduction and cultivation of eucalyptus plants are carried out. The Chengdu Plain is the northernmost region where eucalyptus is planted on a large scale. Nearly 2.7 million mu of giant eucalyptus plantations were once planted. In the era of large-scale planting of giant eucalyptus, nurseries were also in a state of repair in autumn and winter, and no longer produced tissue culture rooted seedlings, nor could they cultivate newly transplanted rooted seedlings. The main reasons are the cold weather and insufficient sunshine. The lower temperature can easily frostbite the newly planted and unlignified seedlings. In addition, the high humidity in the Chengdu Plain makes the seedlings prone to damping-off and mildew. Insufficient light can cause the seedlings to grow too tall and the internodes to lengthen. The height specification of qualified eucalyptus seedlings is 20-50cm. Seedlings exceeding 50cm are considered waste seedlings. Therefore, when the seedlings that grow too tall in winter enter spring, their height can easily exceed 50cm and become waste.

[0006] The method of heating and supplementary lighting can make up for the lack of temperature and light, but the cost is very high and it is not suitable for large-scale batch production. Therefore, giant eucalyptus nurseries in various places will not use the method of heating and supplementary lighting in winter production.

[0007] At present, the production of Eucalyptus grandis tissue culture seedlings is the same as that of Eucalyptus grandis, that is, it will enter the seed preservation stage after the Mid-Autumn Festival, and the rooting seedlings will no longer be produced. In mid-to-late February of the following year, the seed preservation and proliferation seedlings will enter the rooting seedling stage, and after the ground temperature rises, the seedlings will be hardened out of the bottle and transplanted in mid-to-late March.

[0008] Currently, there are no patents or research on the regulation and management of Eucalyptus large-flowered seedlings after transplanting under low temperatures and weak light in autumn and winter in the Chengdu-Chongqing area.

[0009] Plant seedling cultivation in winter requires overcoming problems such as insufficient light and low temperature. It is necessary to ensure that the seedlings do not grow too tall and prevent them from hibernating. The main methods used are physical insulation and chemical treatment. Physical insulation uses heating and plastic film greenhouse insulation. Existing heating and insulation devices consume a lot of energy, increase costs and operational complexity, and are prone to local overheating or uneven heating and cooling. Some simple devices still rely on manual monitoring and adjustment, lack automated temperature and humidity control, and may affect the efficiency and effect of seedling cultivation.

[0010] In the prior art, although some single-arch greenhouses are simple, easy to operate and low-cost, their thermal insulation and natural ventilation capabilities are limited in the Chengdu-Chongqing area where winters are cold and humid. The humidity in the shed is difficult to control, the seedlings are prone to disease, the wettable height of the substrate section is difficult to control, and the root system is difficult to develop uniformly. The purpose of the seedling placement method is only to cut the roots and prevent the roots from entering the soil after penetrating. There is no nutrient solution dedicated to seedlings, especially foliar fertilizers dedicated to winter. Although some double-arch greenhouses have improved the thermal insulation performance and space utilization of single-arch greenhouses to a certain extent, there are still some shortcomings, such as: increased costs, increased management complexity, and double-layer film covering may further reduce light transmittance and affect photosynthesis. Providing supplementary lighting equipment can solve the problem of insufficient light caused by the use of double-layer films, but supplementary lighting equipment increases costs and energy consumption, and its stability, accuracy and adaptability are also difficult to guarantee. The use of chemical agents, such as paclobutrazol and brassinolide, can effectively solve specific growth problems in agriculture, but long-term or excessive use of chemical regulators may accumulate in the soil, affecting the ecological environment and biodiversity, and also reducing the adaptability and resistance of plants themselves. In view of this, the development of new plant winter seedling raising strategies is particularly critical. Summary of the invention

[0011] In view of the above-mentioned deficiencies in the prior art, the present invention provides a factory-based seedling cultivation method for Eucalyptus grandisfolia tissue culture seedlings in the Chengdu-Chongqing area in autumn and winter, which effectively solves the problems of excessive elongation of stem segments of Eucalyptus grandisfolia seedlings in winter, hibernation of Eucalyptus grandisfolia tissue culture seedlings, and susceptibility to damping-off disease due to high humidity.

[0012] To achieve the above purpose, the technical solution adopted by the present invention to solve the technical problem is: to provide a factory-based seedling raising method for Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu and Chongqing, comprising the following steps:

[0013] S1. Transplant the hardened Eucalyptus regia tissue culture seedlings into a completely soaked substrate section for cultivation, and spray winter seedling compound foliar fertilizer during the period;

[0014] S2, placing the container seedlings in a tray, and after no water drips from the tray, placing the tray in a winter seedling raising shed for Eucalyptus regia for cultivation;

[0015] Furthermore, the winter seedling raising shed of the Eucalyptus grandiflora comprises an elevated seedling raising system, a secondary insulation shed and a tertiary insulation shed from the inside to the outside.

[0016] Furthermore, the above-mentioned elevated seedling system includes a basic seedbed, a water-retaining and root-promoting layer and an inner heat-insulating shed.

[0017] The beneficial effects of adopting the above further solution are: the two-layer air cushion buffer structure can keep the temperature in the inner insulation shed stable. On warm sunny days in winter, the temperature can be quickly reduced by retracting the secondary insulation film and opening the skylight of the third-level insulation shed. The multi-layer air cushion can ensure that the temperature of the seedling bed is 3-5℃ higher than the outside.

[0018] Furthermore, in step S1, the humidity of the substrate section is 20-40%.

[0019] Furthermore, in step S1, the compound foliar fertilizer for winter seedling cultivation is sprayed every 3-4 days. After culturing for 28-32 days, or when the seedlings are semi-lignified, the compound foliar fertilizer for winter seedling cultivation is sprayed every 10-11 days.

[0020] Furthermore, in step S1, the compound foliar fertilizer for winter seedling cultivation is sprayed every 3 days. After 30 days of cultivation, or when the seedlings are semi-lignified, the compound foliar fertilizer for winter seedling cultivation is sprayed every 10 days.

[0021] Furthermore, in step S1, the spraying is performed until the leaves are wet and water drips from the leaf tips.

[0022] The beneficial effect of adopting the above further scheme is: to promote lignification of seedling stem segments.

[0023] Further, in step S1, the compound foliar fertilizer for winter seedling cultivation includes the following components in parts by weight: 1-2 parts of revised basal culture medium for tissue culture of Eucalyptus grandiflora, 900-1100 parts of plant extract water-soluble fertilizer and 2800-3200 parts of EDDHA chelated iron.

[0024] Further, in step S1, the compound foliar fertilizer for winter seedling cultivation includes the following components in parts by weight: 1 part of revised basal medium for tissue culture of Eucalyptus grandiflora, 1000 parts of plant extract water-soluble fertilizer and 3000 parts of EDDHA chelated iron.

[0025] Furthermore, the revised basal culture medium for tissue culture of Eucalyptus large inflorescence is prepared by the following steps: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, copper sulfate, cobalt chloride, sodium molybdate, inositol, pyridoxine hydrochloride / pyridoxine hydrochloride, niacin, ascorbic acid, glycine and vitamin B1 are mixed evenly to obtain the revised basal culture medium for tissue culture of Eucalyptus large inflorescence.

[0026] Further, the concentration of potassium nitrate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 550-650mg / L, the concentration of ammonium nitrate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 500-600mg / L, the concentration of potassium dihydrogen phosphate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 250-350mg / L, the concentration of magnesium sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 350-400mg / L, the concentration of calcium chloride in the revised basal medium for tissue culture of Eucalyptus grandiflora is 400-500mg / L, the concentration of potassium iodide in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.5-1mg / L, the concentration of boric acid in the revised basal medium for tissue culture of Eucalyptus grandiflora is 12-13mg / L, the concentration of manganese sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 22-23mg / L, the concentration of zinc sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 8-9mg / L, and the concentration of copper sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 10-15mg / L. The concentration of cobalt chloride in the revised basal culture medium of Eucalyptus grandiflora is 0.02-0.03 mg / L, the concentration of sodium molybdate in the revised basal culture medium of Eucalyptus grandiflora is 0.2-0.3 mg / L, the concentration of inositol in the revised basal culture medium of Eucalyptus grandiflora is 90-110 mg / L, the concentration of pyridoxine / octane hydrochloride in the revised basal culture medium of Eucalyptus grandiflora is 0.2-0.3 mg / L, the concentration of nicotinic acid in the revised basal culture medium of Eucalyptus grandiflora is 0.2-0.3 mg / L, the concentration of ascorbic acid in the revised basal culture medium of Eucalyptus grandiflora is 0.4-0.6 mg / L, the concentration of glycine in the revised basal culture medium of Eucalyptus grandiflora is 1-3 mg / L, and the concentration of vitamin B1 in the revised basal culture medium of Eucalyptus grandiflora is 0.05-0.15 mg / L.

[0027] Furthermore, the concentration of potassium nitrate in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 600 mg / L, the concentration of ammonium nitrate in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 550 mg / L, the concentration of potassium dihydrogen phosphate in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 300 mg / L, the concentration of magnesium sulfate in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 370 mg / L, the concentration of calcium chloride in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 440 mg / L, the concentration of potassium iodide in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 0.83 mg / L, the concentration of boric acid in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 12.4 mg / L, the concentration of manganese sulfate in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 22.3 mg / L, the concentration of zinc sulfate in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 8.6 mg / L, and the concentration of sulfur The concentration of copper phosphate in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 0.025 mg / L, the concentration of cobalt chloride in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 0.025 mg / L, the concentration of sodium molybdate in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 0.25 mg / L, the concentration of inositol in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 110 mg / L, the concentration of pyridoxine / octane hydrochloride in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 0.25 mg / L, the concentration of nicotinic acid in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 0.25 mg / L, the concentration of ascorbic acid in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 0.5 mg / L, the concentration of glycine in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 2 mg / L, and the concentration of vitamin B1 in the revised basal culture medium of Eucalyptus grandiflora tissue culture is 0.1 mg / L.

[0028] Furthermore, the plant-extracted water-soluble fertilizer includes the following components in parts by weight: 20.8-21 parts of organic matter, 1-2 parts of organic nitrogen, 2-3 parts of urea nitrogen, 3-4 parts of potassium oxide, 10-11 parts of organic carbon, 0.2-0.3 parts of molybdenum, 0.5-0.6 parts of manganese and 0.5-0.6 parts of zinc.

[0029] Furthermore, the plant-extracted water-soluble fertilizer includes the following components in parts by weight: 20.8 parts of organic matter, 1 part of organic nitrogen, 2 parts of urea nitrogen, 3 parts of potassium oxide, 10 parts of organic carbon, 0.2 parts of molybdenum, 0.5 parts of manganese and 0.5 parts of zinc.

[0030] The beneficial effects of adopting the above further scheme are: the macroelements in the culture medium are used to promote the normal growth of young leaves, the organic aqueous solution promotes the lignification of stem segments, and the chelated iron increases the chlorophyll content and enhances photosynthesis under short-day conditions.

[0031] Furthermore, in step S2, the tray is a plastic tray with holes, and the hole diameter is 1-2 cm.

[0032] The beneficial effect of adopting the above further scheme is that the root system can penetrate freely.

[0033] Furthermore, the width of the basic seedbed is 1.4-1.6m and 50-80cm from the ground.

[0034] Furthermore, the width of the basic seedbed is 1.5m and it is 50-80cm from the ground.

[0035] Furthermore, a wire mesh is provided on the basic seedbed.

[0036] Further, the spacing of the wire mesh is 5-10cm.

[0037] Furthermore, the material of the water-retaining and root-promoting layer is acrylic cotton, with a thickness of 1.5-2.5 cm, and is arranged on the wire mesh.

[0038] The beneficial effects of adopting the above further scheme are: it can keep warm, moisturize and promote root development.

[0039] Furthermore, the thickness of the water-retaining and root-promoting layer is 2 cm.

[0040] Furthermore, the inner insulation shed includes a PVC frame and a drip-free high-transmittance film.

[0041] Furthermore, the diameter of the PVC skeleton is 20-25 mm.

[0042] Furthermore, PVC vertical columns with a height of 20-30 cm are set every 1.5-2.5 m on both sides of the inner insulation shed.

[0043] Furthermore, the PVC vertical columns are connected using arched PVC pipes.

[0044] Further, the distance between the highest point of the arched PVC pipe and the wire mesh is 40-100 cm.

[0045] Furthermore, the height of the secondary insulation shed is 2-4m.

[0046] Furthermore, the distance between the secondary insulation shed and the arched PVC pipe is 0.5-2.5m.

[0047] The beneficial effect of adopting the above further solution is that it is convenient to operate the inner insulation shed.

[0048] Furthermore, the secondary insulation shed includes a PEP film insulation layer and a herringbone frame.

[0049] Furthermore, manual film rollers are provided on both sides and the top of the secondary insulation shed.

[0050] The beneficial effect of adopting the above further scheme is: it is convenient to roll and ventilate. When the humidity in the inner insulation shed is too high and the temperature is low, after opening the inner insulation film, the space in the second insulation shed can dilute the humidity in the small arch shed and keep warm at the same time.

[0051] Furthermore, the thickness of the PEP film insulation layer is 8-12 filaments thick, and the light transmittance is 85-95%.

[0052] Furthermore, the thickness of the PEP film insulation layer is 10 filaments thick and the light transmittance is 91%.

[0053] Furthermore, the "human" shaped frame is a 4cm×6cm hot-dip galvanized square tube.

[0054] Furthermore, the third-level insulation shed is 2-3m higher than the second-level insulation shed.

[0055] Furthermore, the top of the three-level insulation shed is a thick PO long-life film.

[0056] Further, the thickness of the thick PO long-life film is 0.1-0.14 mm.

[0057] Furthermore, the thickness of the thick PO long-life film is 0.12 mm.

[0058] Furthermore, the third-level insulation shed is fixed and sealed with special clamps, or covered with PC boards with a thickness of 8-10mm or double-layer tempered glass.

[0059] The beneficial effect of adopting the above further solution is that the greenhouse has better air tightness.

[0060] Furthermore, in step S2, the temperature in the greenhouse is 10-20°C during the day and 5-15°C at night.

[0061] Furthermore, when the temperature is too high, the drip-free high-permeability film in the inner insulation shed, the PEP film in the secondary insulation shed, and the skylight in the tertiary insulation shed can be opened in turn to accelerate ventilation and heat dissipation.

[0062] Furthermore, the light intensity is 2000-20000 lux.

[0063] Furthermore, in the Chengdu Plain, there is no need for supplementary lighting in winter, and shade nets are needed to control light intensity on sunny days.

[0064] Furthermore, the greenhouse remains dry and there is no need to clean the ground.

[0065] Furthermore, a hygrometer is hung inside the inner insulation shed.

[0066] Further, the air humidity is 20-60%.

[0067] Furthermore, when the humidity in the greenhouse exceeds 60%, the drip-free high-permeability film in the inner greenhouse must be completely opened to accelerate the volatilization of excess water vapor, prevent excessive water vapor accumulation from causing damping-off disease, and prevent water vapor from condensing into droplets on the film and falling on the seedlings. Especially a few days after watering, when the temperature in the greenhouse is higher than 25°C from noon to 5 pm on a sunny day, the greenhouse skylight, side windows or doors need to be opened.

[0068] The beneficial effect of adopting the above further scheme is: achieving the purpose of rapidly reducing humidity and temperature.

[0069] Furthermore, in step S2, during the cultivation process, water is sprayed upward from the bottom of the seedbed every 10-15 days until the acrylic cotton is completely wetted.

[0070] The beneficial effect of adopting the above further solution is that acrylic cotton is soft and easy for plant roots to penetrate, but its water absorption performance is poor. Water can penetrate to the bottom of the matrix section through acrylic cotton and gradually penetrate upward, forming a humidity gradient of dry top and wet bottom, which is conducive to the development of the root system downward. This can effectively avoid the risk of excessive moisture of crop roots caused by the traditional top-down irrigation method, thereby causing damping-off disease.

[0071] The present invention has the following beneficial effects:

[0072] 1. The method provided by the present invention can effectively prevent the excessive growth of the stem segments of the Eucalyptus ovata seedlings caused by high temperature and low light in the shed in winter, and maintain the slow growth of the stem segments. Temperature control in the elevated seedling system (10-20°C during the day, 5-15°C at night) is more than 10°C away from the critical temperature of 5°C of the hibernation temperature of the Eucalyptus ovata seedlings, which is conducive to the lignification of the stem segments of the Eucalyptus ovata seedlings that are not lignified as soon as possible, and the stem segments after lignification are often in a semi-dormant state, and the 28-35°C required for the normal growth of the Eucalyptus ovata seedlings is far apart, therefore, in the case of a shorter light duration in winter, the stem segments will not grow excessively. Actual measurements found that the Eucalyptus ovata tissue culture rooted seedlings, after hardening and transplanting to the matrix segment, the high growth amount of 1-3 months using the method provided by the present invention is 6-10cm, and the stem segments have a high degree of lignification. After the beginning of spring, transfer to an ordinary shed or outdoor nursery for cultivation, and after 1 month, the seedling height can reach more than 20cm, reaching the afforestation seedling standard.

[0073] 2. The method provided by the present invention can effectively control the humidity of the space above the nursery bed and the direction of condensed water flow. The seedlings in the vertical pole arch shed grow uniformly, completely avoiding the impact of the film squeezing on the seedlings on both sides when the conventional ground film is covered. At the same time, the bottom of the nursery bed is transparent and the air permeability is much higher than that of the conventional ground nursery bed. When there is no watering, no condensed water will be generated on the film. On the day of watering, the generated condensed water will flow down in a linear shape along the two sides of the arched film, and will not become water drops and fall on the leaves of the seedlings. Keep the leaves dry in winter and it is not easy to be infected by bacteria and mold.

[0074] 3. The method provided by the present invention can make the root system abundant and grow vigorously. The acrylic cotton of the water-retaining and root-promoting layer is a key factor in ensuring water supply at the bottom of the matrix section, ensuring the moisture gradient within the matrix section, and root development. It can provide moisture and low-light protection for the penetrating roots to prevent air from cutting the roots. After 3 months of cultivation, the seedlings not only have a large number of fibrous roots, which is equivalent to the number of fibrous roots of seedlings grown in summer, but also the roots are entangled in the matrix section and tightly interwoven with the matrix. White root tips can be seen all around the matrix section. After afforestation, it has good potential for horizontal diffusion all around, which is particularly beneficial to the growth and extension of the root system in the soil, and can greatly improve the afforestation survival rate.

[0075] 4. The unique two-layer air cushion structure of the present invention is the key factor in maintaining the temperature stability in the inner insulation shed, while the inner insulation film and the secondary insulation shed and the tertiary insulation shed can be opened or the skylight structure is the key factor in dissipating the humidity in the inner insulation shed.

[0076] 5. After spraying the winter seedling compound foliar fertilizer provided by the present invention, the leaves of the seedlings are greener and the stem segments are lignified faster. The chlorophyll a and total chlorophyll content of the leaves are significantly higher than those of the untreated control, so that the ability of the plant to capture light energy is increased. With the assistance of organic water-soluble fertilizers, the speed of stem segment lignification is significantly improved. The winter seedling compound foliar fertilizer reduces the nitrogen content to prevent the plant leaves from developing too fast after absorbing too much nitrogen, resulting in excessively large and fat leaves. In addition, the macroelements in the revised basal culture medium of Eucalyptus ovata tissue culture are used to promote the normal growth of young leaves, the organic aqueous solution promotes the lignification of stem segments, and the chelated iron increases the chlorophyll content, enhancing photosynthesis under short-day conditions.

[0077] 6. The method provided by the present invention (1) can prevent and treat damping-off disease caused by high humidity after seedlings are transplanted and before the stem segments become lignified; (2) promote the growth and development of the root system of Eucalyptus regia tissue culture seedlings and the root system of Eucalyptus regia tissue culture seedlings is promoted so that the root system of Eucalyptus regia tissue culture seedlings can be entangled with the substrate and formed into a cluster in the substrate segment; (3) prevent Eucalyptus regia tissue culture seedlings from hibernating in winter, thereby achieving uninterrupted production of Eucalyptus regia tissue culture seedlings in winter; (4) shorten the lignification time of the stem segments, increase the chlorophyll content, increase the leaf thickness, and maintain the normal shape of the leaves. BRIEF DESCRIPTION OF THE DRAWINGS

[0078] Figure 1 This is a real picture of the winter seedling greenhouse for Eucalyptus grandiflora;

[0079] Figure 2 This is a picture of the cultivation of Eucalyptus macrocarpa tissue culture seedlings in the greenhouse;

[0080] Figure 3 This is a real picture of the matrix seedlings of Eucalyptus grandis cultivated on the elevated seedling system. DETAILED DESCRIPTION

[0081] The principles and features of the present invention are described below, and the examples are only used to explain the present invention and are not used to limit the scope of the present invention. If no specific conditions are specified in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be purchased commercially.

[0082] Example 1

[0083] A method for factory-based seedling cultivation of Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area comprises the following steps:

[0084] S1. Transplant the Eucalyptus regia tissue culture seedlings that have been hardened into a completely soaked matrix section for cultivation, spray winter seedling compound foliar fertilizer during the period, spray winter seedling compound foliar fertilizer every 3 days, and after 30 days of cultivation, spray winter seedling compound foliar fertilizer every 10 days. The above-mentioned seasonal seedling compound foliar fertilizer includes the following components in parts by weight: 1 part of the revised basal medium for tissue culture of Eucalyptus regia, 1000 parts of organic water-soluble fertilizer and 3000 parts of EDDHA chelated iron. The preparation method of the above-mentioned revised basal medium for tissue culture of Eucalyptus regia includes the following steps: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, copper sulfate, cobalt chloride, sodium molybdate , inositol, pyridoxine hydrochloride / spicrine, niacin, ascorbic acid, glycine and vitamin B1 are evenly mixed to obtain a revised basal medium for tissue culture of Eucalyptus grandiflora, wherein the concentration of potassium nitrate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 600 mg / L, the concentration of ammonium nitrate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 550 mg / L, the concentration of potassium dihydrogen phosphate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 300 mg / L, the concentration of magnesium sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 370 mg / L, the concentration of calcium chloride in the revised basal medium for tissue culture of Eucalyptus grandiflora is 440 mg / L, and the concentration of potassium iodide in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.83mg / L, the concentration of boric acid in the revised basal medium of Eucalyptus grandiflora tissue culture is 12.4mg / L, the concentration of manganese sulfate in the revised basal medium of Eucalyptus grandiflora tissue culture is 22.3mg / L, the concentration of zinc sulfate in the revised basal medium of Eucalyptus grandiflora tissue culture is 8.6mg / L, the concentration of copper sulfate in the revised basal medium of Eucalyptus grandiflora tissue culture is 0.025mg / L, the concentration of cobalt chloride in the revised basal medium of Eucalyptus grandiflora tissue culture is 0.025mg / L, the concentration of sodium molybdate in the revised basal medium of Eucalyptus grandiflora tissue culture is 0.25mg / L, the concentration of inositol in the revised basal medium of Eucalyptus grandiflora tissue culture is 110mg / L, hydrochloric acid The concentration of pyridoxine / cin in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.25 mg / L, the concentration of nicotinic acid in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.25 mg / L, the concentration of ascorbic acid in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.5 mg / L, the concentration of glycine in the revised basal medium for tissue culture of Eucalyptus grandiflora is 2 mg / L, and the concentration of vitamin B1 in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.1 mg / L. The plant-extracted water-soluble fertilizer comprises the following components in parts by weight: 21.8 parts of organic matter, 1 part of organic nitrogen, 2 parts of urea nitrogen, 3 parts of potassium oxide, 10 parts of organic carbon, 0.2 parts of molybdenum, 0.5 parts of manganese and 0.5 parts of zinc;

[0085] S2. Place the container seedlings in a perforated plastic tray with a hole diameter of 1 cm. When there is no water dripping from the tray, place the tray in the winter seedling shed of Eucalyptus ovata for cultivation. The winter seedling shed includes an elevated seedling system, a secondary insulation shed and a tertiary insulation shed from the inside to the outside. Among them, the elevated seedling system includes a seedbed, a water-retaining and root-promoting layer and an inner insulation shed. The width of the seedbed is 1.5m and the height is 75cm from the ground. A wire mesh is arranged on the seedbed with a spacing of 8cm. The water-retaining and root-promoting layer is acrylic cotton with a thickness of 2cm, which is arranged on the wire mesh. The inner insulation shed includes a frame composed of a PVC pipe with a diameter of 22mm and a drip-free high-transmittance film. There are two sides of the inner insulation shed every 2m. 25cm high vertical PVC pipes, vertical PVC pipes are connected by arched PVC pipes, the top of the arched PVC pipe is 40cm away from the wire mesh, the height of the secondary insulation shed is 3m, 1m away from the arched PVC pipe, including 10-wire thick, 91% transmittance PEP lead film as the insulation layer and a "human" shaped frame with a specification of 4cm×6cm hot-dip galvanized square and rectangular pipes. Manual film rolls are installed on both sides and the top of the secondary insulation shed. The tertiary insulation shed is 2m higher than the secondary insulation shed, with skylights on the top and sides. The top is a 0.12mm thick PO longevity film, which is fixed and sealed with special clamps. The real picture of the winter seedling shed is as follows Figure 1 As shown, in the secondary insulation shed, the daytime temperature is 10-20℃, and the nighttime temperature is 5-15℃. When the temperature is too high, the drip-free high-permeability film in the inner insulation shed, the PEP Lid film in the secondary insulation shed and the skylight in the tertiary insulation shed can be opened in turn to speed up ventilation and heat dissipation. The light intensity is 2000-20000lux. On sunny days, the light intensity needs to be controlled with a sunshade net. The greenhouse is kept dry and there is no need to clean the ground. The air humidity is controlled at 20-60%, and the humidity of the substrate section is controlled at 20-40%. When the humidity in the insulation shed exceeds 60%, the drip-free high-permeability film in the inner insulation shed must be completely opened to speed up the evaporation of excess water vapor. From noon to 5 pm on sunny days, when the temperature in the shed is higher than 25℃, the greenhouse skylight, side windows or doors need to be opened. During the cultivation process, spray water upward from the bottom of the seedbed every 10-15 days until the acrylic cotton is completely moistened.

[0086] Example 2

[0087] A method for factory-based seedling cultivation of Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area comprises the following steps:

[0088] S1. Transplant the Eucalyptus regia tissue culture seedlings that have been hardened into a completely soaked matrix section for cultivation, spray winter seedling compound foliar fertilizer during the period, spray winter seedling compound foliar fertilizer every 3 days, and after culturing for 28 days, spray winter seedling compound foliar fertilizer every 10 days. The above-mentioned seasonal seedling compound foliar fertilizer includes the following components in parts by weight: 1 part of the revised basal medium for tissue culture of Eucalyptus regia, 800 parts of organic water-soluble fertilizers and 2800 parts of EDDHA chelated iron. The preparation method of the above-mentioned revised basal medium for tissue culture of Eucalyptus regia comprises the following steps: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, copper sulfate, cobalt chloride, molybdenum Sodium sulfate, inositol, pyridoxine hydrochloride / spicrine, niacin, ascorbic acid, glycine and vitamin B1 are evenly mixed to obtain a revised basal medium for tissue culture of Eucalyptus grandiflora, wherein the concentration of potassium nitrate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 550 mg / L, the concentration of ammonium nitrate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 500 mg / L, the concentration of potassium dihydrogen phosphate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 250 mg / L, the concentration of magnesium sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 350 mg / L, the concentration of calcium chloride in the revised basal medium for tissue culture of Eucalyptus grandiflora is 400 mg / L, and the concentration of potassium iodide in the revised basal medium for tissue culture of Eucalyptus grandiflora is 100 mg / L. The concentration of 0.5mg / L in the basal medium for tissue culture revision of Eucalyptus grandiflora is 12mg / L, the concentration of manganese sulfate in the basal medium for tissue culture revision of Eucalyptus grandiflora is 22mg / L, the concentration of zinc sulfate in the basal medium for tissue culture revision of Eucalyptus grandiflora is 8mg / L, the concentration of copper sulfate in the basal medium for tissue culture revision of Eucalyptus grandiflora is 0.02mg / L, the concentration of cobalt chloride in the basal medium for tissue culture revision of Eucalyptus grandiflora is 0.02mg / L, the concentration of sodium molybdate in the basal medium for tissue culture revision of Eucalyptus grandiflora is 0.2mg / L, the concentration of inositol in the basal medium for tissue culture revision of Eucalyptus grandiflora is 90mg / L, pyridoxine hydrochloride / cin in the basal medium for tissue culture of Eucalyptus grandiflora is 0.2mg / L, nicotinic acid in the basal medium for tissue culture of Eucalyptus grandiflora is 0.2mg / L, ascorbic acid in the basal medium for tissue culture of Eucalyptus grandiflora is 0.4mg / L, glycine in the basal medium for tissue culture of Eucalyptus grandiflora is 1mg / L, vitamin B1 in the basal medium for tissue culture of Eucalyptus grandiflora is 0.05mg / L, the plant extract water-soluble fertilizer comprises the following components in parts by weight: 20.8 parts of organic matter, 1 part of organic nitrogen, 2 parts of urea nitrogen, 3 parts of potassium oxide, 101 parts of organic carbon, 0.2 parts of molybdenum, 0.5 parts of manganese and 0.5 parts of zinc;

[0089] S2. Place the container seedlings in a perforated plastic tray with a hole diameter of 1 cm. When there is no water dripping from the tray, place the tray in the winter seedling shed of Eucalyptus ovata for cultivation. The winter seedling shed includes an elevated seedling system, a secondary insulation shed and a tertiary insulation shed from the inside to the outside. Among them, the elevated seedling system includes a seedbed, a water-retaining and root-promoting layer and an inner insulation shed. The width of the seedbed is 1.4m and the height is 50cm from the ground. A wire mesh is set on the seedbed with a spacing of 5cm. The water-retaining and root-promoting layer is acrylic cotton with a thickness of 1.5cm, which is set on the wire mesh. The inner insulation The shed includes a frame composed of a 20mm diameter PVC pipe and a drip-free high-transmittance film. A 20cm high vertical PVC pipe is set up every 2m on both sides of the inner insulation shed. The vertical PVC pipes are connected by an arched PVC pipe. The top of the arched PVC pipe is 50cm away from the wire mesh. The height of the secondary insulation shed is 2m, and the distance from the arched PVC pipe is 0.5m. It includes a PEP film with a thickness of 8 wires and a light transmittance of 96% as an insulation layer and a "human" shaped frame with a specification of 4cm×6cm hot-dip galvanized square and rectangular pipes. The side and top films are equipped with manual film rolls. The third-level insulation shed is 3m higher than the second-level insulation shed. There are skylights on the top and sides. The top is a 0.12mm thick PO long-life film, covered with a 9mm thick PC board or double-layer tempered glass. In the second-level insulation shed, the daytime temperature is controlled at 10-20℃, and the night temperature is controlled at 5-15℃. When the temperature is too high, the drip-free high-transmittance film in the inner insulation shed, the PEP Lide film in the second-level insulation shed and the skylight in the third-level insulation shed can be opened in turn to speed up ventilation and heat dissipation. The light intensity is 200 0-20000lux. On sunny days, a sunshade net should be used to control the light intensity. The greenhouse should be kept dry and there is no need to clean the ground. The air humidity should be controlled at 20-60%, and the humidity of the substrate should be controlled at 20-40%. When the humidity in the insulation greenhouse exceeds 60%, the drip-free high-permeability film in the inner insulation greenhouse must be completely opened to accelerate the evaporation of excess water vapor. From noon to 5 pm on sunny days, when the temperature in the greenhouse is higher than 25°C, the greenhouse skylight, side windows or doors need to be opened. During the cultivation process, spray water upward from the bottom of the seedbed every 10-15 days until the acrylic cotton is completely moistened.

[0090] Example 3

[0091] A method for factory-based seedling cultivation of Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area comprises the following steps:

[0092] S1. Transplant the Eucalyptus regia tissue culture seedlings that have been hardened into a completely soaked matrix section for cultivation, spray winter seedling compound foliar fertilizer during the period, spray winter seedling compound foliar fertilizer every 4 days, and after culturing for 32 days, spray winter seedling compound foliar fertilizer every 11 days. The above-mentioned seasonal seedling compound foliar fertilizer includes the following components in parts by weight: 2 parts of Eucalyptus regia tissue culture revised basal culture medium, 1100 parts of organic water-soluble fertilizer and 3200 parts of EDDHA chelated iron. The preparation method of the above-mentioned Eucalyptus regia tissue culture revised basal culture medium includes the following steps: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, copper sulfate, cobalt chloride, Sodium molybdate, inositol, pyridoxine hydrochloride / pyridoxine, niacin, ascorbic acid, glycine and vitamin B1 are evenly mixed to obtain a revised basal medium for tissue culture of Eucalyptus grandiflora, wherein the concentration of potassium nitrate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 650 mg / L, the concentration of ammonium nitrate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 600 mg / L, the concentration of potassium dihydrogen phosphate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 350 mg / L, the concentration of magnesium sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 400 mg / L, the concentration of calcium chloride in the revised basal medium for tissue culture of Eucalyptus grandiflora is 500 mg / L, and the concentration of potassium iodide in the revised basal medium for tissue culture of Eucalyptus grandiflora is 150 mg / L. The concentration in the culture medium is 1mg / L, the concentration of boric acid in the revised basal medium for tissue culture of Eucalyptus grandiflora is 13mg / L, the concentration of manganese sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 23mg / L, the concentration of zinc sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 9mg / L, the concentration of copper sulfate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.03mg / L, the concentration of cobalt chloride in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.03mg / L, the concentration of sodium molybdate in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.3mg / L, the concentration of inositol in the revised basal medium for tissue culture of Eucalyptus grandiflora is 110mg / L, and the concentration of pyridoxine hydrochloride in the revised basal medium for tissue culture of Eucalyptus grandiflora is 100mg / L. The concentration of pyridoxine / oxadiazole in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.3 mg / L, the concentration of nicotinic acid in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.3 mg / L, the concentration of ascorbic acid in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.6 mg / L, the concentration of glycine in the revised basal medium for tissue culture of Eucalyptus grandiflora is 3 mg / L, and the concentration of vitamin B1 in the revised basal medium for tissue culture of Eucalyptus grandiflora is 0.15 mg / L. The plant-extracted water-soluble fertilizer comprises the following components in parts by weight: 21 parts of organic matter, 2 parts of organic nitrogen, 3 parts of urea nitrogen, 4 parts of potassium oxide, 11 parts of organic carbon, 0.3 parts of molybdenum, 0.6 parts of manganese and 0.6 parts of zinc;

[0093] S2. Place the container seedlings in a perforated plastic tray with a hole diameter of 1 cm. After no water drips from the tray, place the tray in the winter seedling shed of Eucalyptus ovata for cultivation. The winter seedling shed includes an elevated seedling system, a secondary insulation shed and a tertiary insulation shed from the inside to the outside. The elevated seedling system includes a seedbed, a water-retaining and root-promoting layer and an inner insulation shed. The width of the seedbed is 1.6 m and the height is 80 cm from the ground. A wire mesh is set on the seedbed with a spacing of 10 cm. The water-retaining and root-promoting layer is acrylic cotton with a thickness of 2.5 cm and is set on the wire mesh. The inner insulation shed includes a frame composed of a 25mm diameter PVC pipe and a drip-free high-transmittance film. A 30cm high vertical PVC pipe is set up every 2m on both sides of the inner insulation shed. The vertical PVC pipes are connected by an arched PVC pipe. The top of the arched PVC pipe is 60cm away from the wire mesh. The height of the secondary insulation shed is 4m, 2.5m away from the arched PVC pipe, including a 12-wire thick, 95% transmittance PEP Lid film as an insulation layer and a "human" shaped frame constructed of 5cm×7cm hot-dip galvanized square and rectangular pipes. The two sides and top film of the secondary insulation shed are equipped with manual film rolls. The third insulation shed is 4m higher than the second insulation shed. There are skylights on the top and sides. The top is a 0.14mm thick PO long-life film, which is fixed and sealed with special clamps. In the secondary insulation shed, the daytime temperature is controlled at 10-20℃, and the night temperature is controlled at 5-15℃. When the temperature is too high, the drip-free high-transmittance film in the inner insulation shed, the PEP Lide film in the secondary insulation shed and the skylight in the tertiary insulation shed can be opened in turn to speed up ventilation and heat dissipation. The light intensity is 2000-2 0000lux, on sunny days, a sunshade net should be used to control the light intensity, the greenhouse should be kept dry, there is no need to clean the ground, the air humidity should be controlled at 20-60%, and the humidity of the substrate should be controlled at 20-40%. When the humidity in the insulation greenhouse exceeds 60%, the drip-free high-permeability film in the inner insulation greenhouse must be completely opened to accelerate the evaporation of excess water vapor. From noon to 5 pm on sunny days, when the temperature in the greenhouse is higher than 25°C, the greenhouse skylight, side windows or doors need to be opened. During the cultivation process, spray water upward from the bottom of the seedbed every 10-15 days until the acrylic cotton is completely moistened.

[0094] Test Example 1

[0095] (1) Screening of basic nutrient solution

[0096] The basic nutrient solution was the tissue culture medium of Eucalyptus ovata, MS medium, WPM medium and the revised basic medium of Eucalyptus ovata, and pure water was used as the control (CK). The composition formula of each basic culture solution is shown in Table 1. After the tissue culture seedlings of Eucalyptus ovata were transplanted to the substrate section, the seedlings were treated with the above-mentioned different foliar fertilizers, 50 seedlings per treatment, and repeated 3 times. After 2 months, the average length and average thickness of the third and fourth leaves of each seedling were counted, and the degree of lignification of the stem segments of the seedlings was counted every 5 days. The results are shown in Table 2.

[0097] Table 1 Basic nutrient solution composition formula

[0098]

[0099] Table 2 Experimental effects of different foliar fertilizers

[0100]

[0101]

[0102] As shown in Table 1, the growth performance of Eucalyptus glomerata seedlings treated with different foliar fertilizers was quite different. From the perspective of seedling morphology, the overall performance of CK seedlings was relatively weak. The seedlings in treatments 3 and 4 were the most symmetrical, while the leaves of seedlings in treatments 1 and 2 were too large. The leaves of seedlings in treatments with high nutrient content were large and thick. The leaf lengths of the four foliar fertilizer treatments were significantly higher than those of CK, the leaf thickness of three treatments was significantly higher than that of CK, and the leaf chlorophyll content of two treatments was significantly higher than that of CK.

[0103] The specific performances of each treatment are as follows: Treatment 1 was derived from the basic culture medium components of Eucalyptus grandiflora tissue culture seedlings, which had a significant effect on the growth of seedling leaves. It was second only to the MS culture medium nutrient solution of Treatment 2, but the chlorophyll content was not significantly different from that of CK, indicating that this treatment had little effect on the increase of chlorophyll content.

[0104] Treatment 2 was derived from MS medium. Since MS medium is the most element-rich formula among all basic mediums, its nitrogen content is more than twice that of other treatments. The results showed that the leaves in this treatment were the largest and thickest, some leaves showed unnatural proliferation, and the middle part was curled or wavy; the leaf color was equivalent to CK, and the chlorophyll content was significantly higher than the control, but the chlorophyll content of 2 was not significantly different from that of other treatments. It is speculated that the reason is that the high nitrogen and ammonia content of MS medium leads to vigorous leaf growth, while its relatively rich organic matter and divalent iron ensure the normal chlorophyll content.

[0105] Treatment 3 was derived from WPM medium, with leaf length of 3.5 cm and average thickness of 0.28 mm, significantly higher than the control, but significantly lower than treatments 2 and 4; chlorophyll content was 0.31 mg / g. At this time, the seedling morphology was better than that of CK.

[0106] Treatment 4 was based on the performance of the seedlings in the above treatments, and the substance content in treatment 1 was adjusted, mainly by reducing the content of nitrogen and other macroelements. Its effect on leaf length growth was equivalent to that of treatment 3, but the leaf thickness was thicker and the frost resistance was better. The chlorophyll content was slightly higher than CK, which was equivalent to treatments 1 and 3.

[0107] Judging from the lignification rate of seedling stem segments, the treatments of spraying basal fertilizer on leaf area are not conducive to seedling lignification and are not beneficial to the winter growth of seedlings.

[0108] In summary, treatment 4 is the preferred basic nutrient solution for foliar fertilizer. However, if it is necessary to increase the lignification rate of seedlings and the content of chlorophyll, the next step of foliar fertilizer experiment is designed accordingly.

[0109] (2) Screening of compound foliar fertilizer for winter seedling cultivation

[0110] Different foliar fertilizers were prepared by adding different water-soluble fertilizers and iron salts to the revised basal medium of Eucalyptus ovata tissue culture in treatment 4. The formulas of each water-soluble fertilizer and iron salt are shown in Table 3 to screen the combination types that can accelerate the lignification and chlorophyll content of the stem segments of Eucalyptus ovata seedlings. After the Eucalyptus ovata tissue culture seedlings were transplanted to the substrate segment, the seedlings were treated with different foliar fertilizers, with 50 seedlings per treatment and repeated 3 times. After 2 months, the average length and average thickness of the third and fourth leaves of each seedling were counted, and the degree of lignification of the stem segments of the seedlings was counted every 2 days. The concentration of organic water-soluble fertilizers and the experimental results are shown in Table 4. The experimental data were analyzed by one-way analysis of variance and Tukey's multiple comparisons.

[0111] Table 3 Composition formula of each water-soluble fertilizer and iron salt

[0112]

[0113]

[0114] Table 4 Effects of different combinations of foliage and fertilizer application on tissue culture seedlings of Eucalyptus ovata

[0115]

[0116]

[0117] As shown in Table 4, 4 kinds of water-soluble fertilizers mainly affect blade length, blade width and lignification time, among which organic water-soluble fertilizer B has the most obvious promoting effect on blade length, compared with the blades of other combinations, the blades processed by organic water-soluble fertilizer B are longer, although its chlorophyll content is also higher (processing 3 is compared with processing 1, and the difference in chlorophyll content is not significant), but it also means that the seedling leaf expansion area is larger, and more area is needed to place seedlings, such as the nursery land of the same area, processing 3 is less than processing 1. Place 13.5%. Organic water-soluble fertilizer C is slightly lower than organic water-soluble fertilizer B in the effect of blade growth, but is still better than plant extraction water-soluble fertilizer. The blade effect of organic water-soluble fertilizer A is not obvious, which is equivalent to processing 4 in Table 2, but after applying organic water-soluble fertilizer A, the slower the stem lignification, the slower the stem growth. It is not the effect required by the present invention.

[0118] The combination of different water-soluble fertilizers and iron salts had significant effects on leaf traits and stem lignification time. Treatment 5 was most conducive to leaf length growth, treatments 2 and 3 were most conducive to leaf thickening, and the leaf green content in treatment 2 was the highest and significantly higher than the other combinations. Treatment 2 had the shortest time required for stem lignification.

[0119] On the whole, treatment 2 had the best effect, which promoted leaf length and thickness to a certain extent, significantly increased chlorophyll content, and significantly shortened lignification time.

[0120] Test Example 2

[0121] During the cultivation process of Example 1, the growth of the Eucalyptus ovata seedlings was observed and recorded. The cultivation of the Eucalyptus ovata tissue culture seedlings in the shed was as follows: Figure 2 The growth of Eucalyptus macrocarpa seedlings grown on the elevated seedling system is shown in Figure 3 As shown, Figure 3 In the middle, from left to right are the substrate segments of Eucalyptus grandis seedlings at 3 months, 2 months and 1 month of cultivation.

[0122] Depend on Figure 2 and Figure 3 It can be known that under the environmental control such as temperature, light and humidity provided by the present invention and the water and fertilizer regulation mode, the root system of the Eucalyptus glomerata seedlings in the greenhouse grows well, and the stem segment can also maintain a lower growth rate. The root system develops to the middle section of the matrix segment at 30d, and the stem segment completes lignification, and the seedling height is 4-6cm. During 60d, the root system completes the wrapping of the entire matrix segment, and white fibrous roots are visible at the bottom of the matrix segment, and the stem segment grows 1-2cm tall, and there are 1-2 pairs of new leaves. During 90d, a large amount of white fibrous roots are visible on the sides and bottom of the matrix segment, and many pairs of new leaves are grown again, and the seedling height is 10-15cm. This type of light matrix seedling is transferred to the open air nursery in spring and cultivated for 10-15d, and can be out of the nursery.

[0123] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for factory-based seedling cultivation of Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area, characterized in that: The following steps are involved: S1. Transplant the hardened Eucalyptus regia tissue culture seedlings into a completely soaked substrate section for cultivation, and spray winter seedling compound foliar fertilizer during the period; S2, placing the container seedlings in a tray, and after no water drips from the tray, placing the tray in a winter seedling raising shed for Eucalyptus regia for cultivation; The winter seedling raising shed of Eucalyptus grandiflora includes an elevated seedling raising system, a secondary insulation shed and a tertiary insulation shed from the inside to the outside; The elevated seedling raising system comprises a basic seedling bed, a water-retaining and root-promoting layer and an inner heat-insulating shed.

2. The method for raising seedlings of Eucalyptus globulus in autumn and winter in Chengdu-Chongqing area according to claim 1, characterized in that: In step S1, the compound foliar fertilizer for winter seedling raising includes the following components in parts by weight: 1-2 parts of revised basal medium for tissue culture of Eucalyptus grandiflora, 900-1100 parts of plant extract water-soluble fertilizer and 2800-3200 parts of EDDHA chelated iron.

3. The method for raising seedlings of Eucalyptus globulus in autumn and winter in Chengdu-Chongqing area in factory scale according to claim 2, characterized in that: The revised basal culture medium for tissue culture of Eucalyptus grandiflora is prepared by the following steps: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, copper sulfate, cobalt chloride, sodium molybdate, inositol, pyridoxine hydrochloride / pyridoxine hydrochloride, nicotinic acid, ascorbic acid, glycine and vitamin B1 are uniformly mixed to obtain the revised basal culture medium for tissue culture of Eucalyptus grandiflora.

4. The method for raising seedlings of Eucalyptus ovata in autumn and winter in Chengdu-Chongqing area according to claim 2, characterized in that: The plant-extracted water-soluble fertilizer comprises the following components in parts by weight: 20.8-21 parts of organic matter, 1-2 parts of organic nitrogen, 2-3 parts of urea nitrogen, 3-4 parts of potassium oxide, 10-11 parts of organic carbon, 0.2-0.3 parts of molybdenum, 0.5-0.6 parts of manganese and 0.5-0.6 parts of zinc.

5. The method for factory-based seedling cultivation of Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area as claimed in claim 1, characterized in that: The width of the basic seedbed is 1.4-1.6m, and the distance from the ground is 50-80cm. A wire mesh is arranged on the basic seedbed, and the spacing between the wire meshes is 5-10cm. The material of the water-retaining and root-promoting layer is acrylic cotton, with a thickness of 1.5-2.5cm, and is arranged on the wire mesh.

6. The method for factory-based seedling cultivation of Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area as claimed in claim 1, characterized in that: The inner insulation shed includes a PVC frame and a drip-free high-transmittance film, and the diameter of the PVC frame is 20-25 mm; PVC vertical columns with a height of 20-30 cm are arranged at intervals of 1.5-2.5 m on both sides of the inner insulation shed. The PVC vertical columns are connected by arched PVC pipes, and the distance between the highest point of the top of the arched PVC pipe and the wire mesh is 40-100 cm.

7. The method for raising seedlings of Eucalyptus ovata in autumn and winter in Chengdu-Chongqing area according to claim 1, characterized in that: The secondary insulation shed includes a PEP film insulation layer and a "human" shaped frame; the height of the secondary insulation shed is 2-4m, and the distance from the arched PVC pipe is 0.5-2.5m; manual film rolls are provided on both sides and the top of the secondary insulation shed; The thickness of the PEP film insulation layer is 8-12 threads thick, and the light transmittance is 85-95%; The "human" shaped frame is a hot-dip galvanized square tube of 4-5cm×6-7cm.

8. The method for raising seedlings of Eucalyptus globulus in autumn and winter in Chengdu-Chongqing area in factory scale according to claim 1, characterized in that: The third-level insulation shed is 2-3m higher than the second-level insulation shed, and is provided with skylights on the top and sides. The top of the third-level insulation shed is a thick PO long-life film, and the thickness of the thick PO long-life film is 0.1-0.14mm.

9. The method for factory-based seedling cultivation of Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area as claimed in claim 1 or 8, characterized in that: The three-level insulation shed is fixed and sealed with special clamps, or covered with a PC board with a thickness of 8-10mm or double-layer tempered glass.

10. The method for factory-based seedling cultivation of Eucalyptus globulus tissue culture seedlings in autumn and winter in Chengdu-Chongqing area according to claim 1, characterized in that: In step S2, the temperature in the greenhouse is 10-20°C during the day, 5-15°C at night, the light intensity is 2000-20000 lux, and the air humidity is 20-60%.

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