Cucumber seed coating agent, preparation method and application

Abstract

The invention provides a cucumber seed coating agent, a preparation method and application, and belongs to the technical field of crop seed coating agents. The cucumber seed coating agent comprises the following active ingredients in percentage by weight: 0.3%-0.5% of sodium hypochlorite, 0.006%-0.01% of chlormequat chloride, 0.012%-0.018% of boric acid and inactive ingredients. Cucumber seeds are coated through the combination of sodium hypochlorite, chlormequat chloride and boric acid, germination of the cucumber seeds can be promoted, the germination rate and germination potential of the cucumber seeds can be improved, the leaf area and chlorophyll content of cucumber seedlings can be increased, excessive growth of the seedlings can be inhibited, and strong seedlings can be cultivated.

Description

Technical Field

[0001] The invention belongs to the technical field of crop seed coating agents, and in particular relates to a cucumber seed coating agent, a preparation method and application thereof. Background Art

[0002] Cucumber is one of the main edible vegetables in my country and plays an important role in the daily life of the people. Its cultivation area accounts for more than 15% of the vegetable cultivation area. However, in the cucumber production process, there are still prominent problems such as low seed vitality, long-legged seedlings, and serious bacterial diseases. These problems not only affect the cucumber yield, but also affect the efficiency and accuracy of the experiment, and need to be solved urgently.

[0003] The promotion of composite seed coating agents provides an effective solution to the above problems. Seed coating agents are compound seed coating products made mainly from plant growth regulators, trace elements, film-forming agents and slow-release agents. It can make the agent evenly and firmly adhere to the seeds and release it gradually, prolonging the effective period of the agent, saving fertilizer, cultivating strong seedlings and increasing yields. At present, there are few seed coating agents specifically suitable for cucumber seeds, and most of them are formulated for field crops. Due to the different habits of crops, the concentration of applicable seed coating agents is also different, so the phenomenon of inhibiting the vitality of cucumbers has always existed. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a cucumber seed coating agent, a preparation method and an application thereof, which can promote cucumber seed germination and have the function of strengthening seedlings.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] A cucumber seed coating agent comprises, by mass concentration, active ingredients of 0.3%-0.5% sodium hypochlorite, 0.006%-0.01% chlormequat chloride, 0.012%-0.018% boric acid and inactive ingredients.

[0007] Preferably, the active ingredients include, by mass concentration, 0.3% sodium hypochlorite, 0.01% chlormequat chloride and 0.018% boric acid; or 0.5% sodium hypochlorite, 0.01% chlormequat chloride and 0.012% boric acid; or one of 0.5% sodium hypochlorite, 0.01% chlormequat chloride and 0.018% boric acid.

[0008] Preferably, the inactive ingredients include dispersants, antifreeze agents, anticaking agents, film formers, preservatives, warning colors, fillers and water.

[0009] More preferably, among the inactive ingredients, the mass ratio of dispersant, antifreeze agent, anticaking agent, film former, preservative, warning color and filler is 40-60:0.8-1.2:0.8-1.2:40-60:1.6-2.4:80-120:8-12.

[0010] More preferably, the dispersant is selected from one or more of sodium lignin sulfonate, pull open powder BX, and sodium N-methyl-fatty acyl-taurate;

[0011] The antifreeze agent is selected from one or more of ethylene glycol, propylene glycol and glycerol;

[0012] The anticaking agent is selected from one or more of silicon dioxide, talc, and magnesium stearate;

[0013] The film-forming agent is selected from one or more of polyvinyl alcohol, sodium carboxymethyl cellulose, and sodium polyacrylate;

[0014] The preservative is selected from one or more of sodium benzoate, potassium sorbate, and sodium dehydroacetate;

[0015] The warning color is selected from one or more of acid scarlet, amaranth, and carmine;

[0016] The filler is selected from one or more of light calcium carbonate, white carbon black and kaolin.

[0017] The present invention also provides a method for preparing a cucumber seed coating agent, comprising the following steps:

[0018] (1) Weigh the dispersant, film-forming agent and preservative in proportion, add water and stir evenly;

[0019] (2) Weigh the warning color and dissolve it in water;

[0020] (3) Weighing an anticaking agent, an antifreeze agent and a filler, together with the warning color solution of step (2), add them to the mixed solution of step (1), and stir evenly to obtain an inactive ingredient slurry;

[0021] (4) According to the concentration, active ingredients sodium hypochlorite, chlormequat chloride and boric acid are added to the slurry of the inactive ingredients to obtain a cucumber seed coating agent.

[0022] The invention also provides application of the cucumber seed coating agent to cucumber seed treatment.

[0023] Preferably, the cucumber seed coating agent is mixed with cucumber seeds at a mass ratio of 1:30-50, and the seeds are soaked and coated; more preferably, after the seeds are soaked and coated, the seeds are fished out and dried to obtain coated cucumber seeds.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The invention provides a cucumber seed coating agent. Sodium hypochlorite, chlormequat chloride and boric acid are used as active ingredients, which are combined to prepare the seed coating agent for coating cucumber seeds, thereby promoting cucumber seed germination, improving germination potential, germination rate and germination index; in the growth period of cucumber seedlings, the agent has a good promoting effect on indicators such as leaf area and chlorophyll content of the cucumber seedlings, and can inhibit excessive growth of the seedlings and cultivate strong seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 :Effects of sodium hypochlorite seed coating on the height of cucumber seedlings;

[0027] Figure 2 :Effects of sodium hypochlorite seed coating on stem diameter of cucumber seedlings;

[0028] Figure 3 :Effects of sodium hypochlorite seed coating on chlorophyll content in cucumber seedlings;

[0029] Figure 4 :Effects of chlormequat seed coating agent on plant height of cucumber seedlings;

[0030] Figure 5 :Effects of chlormequat seed coating agent on stem diameter of cucumber seedlings;

[0031] Figure 6 :Effects of chlormequat seed coating agent on chlorophyll content in cucumber seedlings;

[0032] Figure 7 :Effects of boric acid seed coating on the height of cucumber seedlings;

[0033] Figure 8 :Effects of boric acid seed coating on stem diameter of cucumber seedlings;

[0034] Fig. 9 : Effects of boric acid seed coating on chlorophyll content in cucumber seedlings. DETAILED DESCRIPTION

[0035] The invention provides a cucumber seed coating agent, which comprises, by mass concentration, 0.3%-0.5% of sodium hypochlorite, 0.006%-0.01% of chlormequat chloride, 0.012%-0.018% of boric acid and inactive ingredients.

[0036] Sodium hypochlorite can effectively inhibit the growth of plant pathogenic bacteria. In the present invention, cucumber seeds are treated with sodium hypochlorite seed coating agent, and it is found that sodium hypochlorite has the effect of promoting the growth of cucumber seedling leaf area and chlorophyll content, which meets the requirements of agricultural production.

[0037] Chlormequat can effectively prevent and control the phenomenon of excessive growth of cucumber seedlings. In the present invention, cucumber seeds are treated with chlormequat seed coating agent, and it is found that chlormequat can promote the late growth of cucumber seedlings and increase the chlorophyll content of plants.

[0038] Boric acid can effectively improve the germination potential and chlorophyll content of cucumber. In the present invention, by treating cucumber seeds with a boric acid seed coating agent, it is found that boric acid can effectively improve the germination rate and germination potential of cucumber.

[0039] The present invention combines sodium hypochlorite, chlormequat and boric acid to prepare a seed coating agent, which is used to coat cucumber seeds, and can promote cucumber seed germination, improve germination potential, germination rate and germination index; during the growth period of cucumber seedlings, it has a good promoting effect on indicators such as leaf area and chlorophyll content of cucumber seedlings, and can inhibit seedling leggy growth and cultivate strong seedlings. The three active ingredients have different concentrations, and the effects on cucumber seed germination and seedling growth are also different. The preferred active ingredient concentration of the present invention is 0.3% sodium hypochlorite, 0.01% chlormequat and 0.018% boric acid; or 0.5% sodium hypochlorite, 0.01% chlormequat and 0.012% boric acid; or one of 0.5% sodium hypochlorite, 0.01% chlormequat and 0.018% boric acid.

[0040] The preferred inactive ingredients of the present invention include dispersants, antifreeze agents, anticaking agents, film formers, preservatives, warning colors, fillers and water. The mass ratio of dispersants, antifreeze agents, anticaking agents, film formers, preservatives, warning colors and fillers is further preferably 40-60:0.8-1.2:0.8-1.2:40-60:1.6-2.4:80-120:8-12; the more preferred ratio is 50:1:1:50:2:100:10.

[0041] The preferred dispersant of the present invention is selected from one or more of sodium lignin sulfonate, pull-open powder BX, and sodium N-methyl-fatty acyl-taurate; the antifreeze agent is selected from one or more of ethylene glycol, propylene glycol, and glycerol; the anticaking agent is selected from one or more of silicon dioxide, talc, and magnesium stearate; the film-forming agent is selected from one or more of polyvinyl alcohol, sodium carboxymethyl cellulose, and sodium polyacrylate; the preservative is selected from one or more of sodium benzoate, potassium sorbate, and sodium dehydroacetate; the warning color is selected from one or more of acid scarlet, amaranth, and carmine; the filler is selected from one or more of light calcium carbonate, white carbon black, and kaolin. The present invention has no special restrictions on the source of each inactive ingredient, and it can be directly obtained through commercial channels.

[0042] The present invention also provides a method for preparing a cucumber seed coating agent, comprising the following steps: (1) weighing a dispersant, a film-forming agent and a preservative in proportion, adding water and stirring evenly; (2) weighing a warning color, adding water and dissolving it; (3) weighing an anticaking agent, an antifreeze agent and a filler, and adding them together with the warning color solution of step (2) to the mixed solution of step (1), stirring evenly, to obtain an inactive ingredient slurry; (4) adding active ingredients of sodium hypochlorite, chlormequat chloride and boric acid to the inactive ingredient slurry according to the concentration, to obtain a cucumber seed coating agent.

[0043] The present invention also provides the use of a cucumber seed coating agent for treating cucumber seeds. Preferably, the cucumber seed coating agent is mixed with the cucumber seeds in a mass ratio of 1:30-50, more preferably 1:40, and the seeds are soaked and coated; more preferably, after the coating is completed, the seeds are fished out and dried to obtain coated cucumber seeds. As an implementation method, after the seed coating is completed, the coated seeds are placed in a blast dryer and dried at 30°C-40°C for 2-4h, and when the seeds are completely dried, they are placed in a cool and dry place for storage.

[0044] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0045] Unless otherwise specified, the methods used in the embodiments of the present invention are conventional methods, and the materials and reagents used can be obtained through commercial channels.

[0046] Example 1

[0047] A cucumber seed coating agent is prepared by the following ingredients and steps:

[0048] Raw materials: sodium hypochlorite, chlormequat chloride, boric acid, sodium lignin sulfonate, ethylene glycol, silicon dioxide, polyvinyl alcohol, sodium benzoate, acid scarlet, light calcium carbonate;

[0049] (1) Weigh 5 g of sodium lignin sulfonate, 0.1 g of polyvinyl alcohol, and 0.1 g of sodium benzoate in proportion, pour into a 200 ml beaker, add appropriate amount of distilled water, and stir evenly;

[0050] (2) Weigh 5g of acid scarlet, add appropriate amount of distilled water and stir

[0051] (3) Weigh 0.2 g of silicon dioxide, 10 g of light calcium carbonate, and 1 g of ethylene glycol, and add them together with the acidic red solution of step (2) to the mixed solution of step (1), and stir evenly to obtain an inactive ingredient slurry;

[0052] (4) According to the formula of 0.3% sodium hypochlorite, 0.010% chlormequat chloride and 0.018% boric acid, active ingredients sodium hypochlorite, chlormequat chloride and boric acid are added to the slurry of inactive ingredients to obtain a cucumber seed coating agent.

[0053] Example 2

[0054] A cucumber seed coating agent is prepared by the following ingredients and steps:

[0055] Raw materials: sodium hypochlorite, chlormequat chloride, boric acid, BX powder, propylene glycol, talc, sodium carboxymethyl cellulose, potassium sorbate, amaranth, and white carbon black.

[0056] (1) Weigh 5 g of BX powder, 0.1 g of sodium carboxymethyl cellulose, and 0.1 g of potassium sorbate in proportion, pour into a 200 ml beaker, add appropriate amount of distilled water, and stir evenly;

[0057] (2) Weigh 5 g of amaranth, add appropriate amount of distilled water and stir;

[0058] (3) Weigh 0.2 g of talc, 10 g of white carbon black, and 1 g of propylene glycol, and add them together with the amaranth solution of step (2) to the mixed solution of step (1), and stir evenly to obtain an inactive ingredient slurry;

[0059] (4) According to the formula of 0.5% sodium hypochlorite, 0.010% chlormequat chloride and 0.012% boric acid, active ingredients sodium hypochlorite, chlormequat chloride and boric acid were added to the inactive ingredient slurry to obtain a cucumber seed coating agent.

[0060] Example 3

[0061] A cucumber seed coating agent is prepared by the following ingredients and steps:

[0062] Ingredients: sodium hypochlorite, chlormequat chloride, boric acid, sodium N-methyl-fatty acyl-taurate, glycerin, magnesium stearate, sodium polyacrylate, sodium dehydroacetate, carmine, kaolin.

[0063] (1) Weigh 5 g of sodium N-methyl-fatty acyl-taurate, 0.1 g of sodium polyacrylate, and 0.1 g of sodium dehydroacetate in proportion, pour into a 200 ml beaker, add appropriate amount of distilled water, and stir evenly;

[0064] (2) Weigh 5 g of carmine, add appropriate amount of distilled water and stir;

[0065] (3) Weighing 0.2 g of magnesium stearate, 10 g of kaolin, and 1 g of glycerin, together with the carmine solution prepared in step (2), add the mixture to the mixture in step (1), and stir evenly to obtain an inactive ingredient slurry;

[0066] (4) According to the formula of 0.5% sodium hypochlorite, 0.010% chlormequat chloride and 0.018% boric acid, active ingredients sodium hypochlorite, chlormequat chloride and boric acid were added to the inactive ingredient slurry to obtain a cucumber seed coating agent.

[0067] Example 4

[0068] A method for coating cucumber seeds comprises the following steps:

[0069] The cucumber seeds were mixed with the coating agent of Example 1 at a mass ratio of 1:40, and the seeds were soaked and coated. After the coating was completed, the seeds were dried in a blast drying oven. When the seeds were completely dried, coated cucumber seeds were obtained.

[0070] Example 5

[0071] The difference from Example 4 is that the mass ratio of cucumber seeds to coating agent is 1:30.

[0072] Example 6

[0073] The difference from Example 4 is that the mass ratio of cucumber seeds to coating agent is 1:50.

[0074] Example 7

[0075] Effects of different active ingredients and contents of cucumber seed coating agents on cucumber seed germination and seedling growth

[0076] 1. Test materials:

[0077] 1.1.Cucumber seeds;

[0078] 1.2. Active substances: sodium hypochlorite NaClO, chlormequat chloride C5H 13 Cl2N, boric acid H3BO3;

[0079] 1.3. Inactive substances: sodium lignin sulfonate, ethylene glycol, silicon dioxide, polyvinyl alcohol, sodium benzoate, acid scarlet, light calcium carbonate.

[0080] 2. Preparation of inactive substances for cucumber seed coating

[0081] 2.1. Weigh 5g sodium lignin sulfonate, 0.1g polyvinyl alcohol, and 0.1g sodium benzoate, pour into a 200ml beaker, add appropriate amount of distilled water, and stir evenly.

[0082] 2.2. Weigh 5g of acid scarlet, add appropriate amount of distilled water and stir

[0083] 2.3. Pour acid scarlet into a 200ml beaker, and weigh 0.2g of silicon dioxide, 10g of light calcium carbonate, and 1g of ethylene glycol and stir evenly.

[0084] 3. Prepare cucumber seed coating agent

[0085] 3.1. Sodium hypochlorite seed coating agent: Add sodium hypochlorite solution to the prepared inactive substance slurry, stir evenly, and prepare 5 kinds of cucumber seed coating agents with concentrations of 0.2%, 0.3%, 0.4%, 0.5%, and 0.6% respectively;

[0086] 3.2. Chlormequat seed coating agent: Add the chlormequat solution to the prepared inactive substance slurry, stir evenly, and prepare 5 kinds of cucumber seed coating agents with concentrations of 0.004%, 0.006%, 0.008%, 0.010%, and 0.012% respectively;

[0087] 3.3. Boric acid seed coating agent: Add the boric acid solution to the prepared inactive substance slurry, stir evenly, and prepare 5 kinds of cucumber seed coating agents with concentrations of 0.006%, 0.012%, 0.018%, 0.024%, and 0.030% respectively;

[0088] 3.4. Sodium hypochlorite / chlormequat / boric acid composite seed coating agent: Add sodium hypochlorite solution, chlormequat solution and boric acid solution to the prepared inactive substance slurry, stir evenly, and prepare 27 kinds of (sodium hypochlorite 0.3%, 0.4%, 0.5%; chlormequat 0.006%, 0.008%, 0.010%; boric acid 0.006%, 0.012%, 0.018%) composite cucumber seed coating agents.

[0089] 4. Cucumber seed coating treatment

[0090] 4.1. Coat the cucumber seeds with the prepared seed coating agent at a ratio of 1:40. After coating, dry the seeds in a forced air drying oven. After the seeds are completely dry, store them in a cool and dry place.

[0091] 4.2. Prepare uncoated seeds as blank control (CK).

[0092] 5. Germination and seedling raising of cucumber seeds

[0093] 5.1. Experiment on cucumber seeds at germination stage with different coating treatments

[0094] First, use a cleaned and dried petri dish as a germination bed, pad the dish with two layers of filter paper, then place the cucumber seeds evenly on the germination bed, and put the dish into a constant temperature germination box at 25℃±10℃ for germination test. Each group of seed coating agent was treated with 20 seeds, and repeated 3 times.

[0095] Cucumber seed germination test:

[0096] In the germination experiment, the germination of cucumber seeds was observed and counted every 24 hours. The germination potential of the seeds was calculated on the 3rd day, and the germination rate of the seeds was calculated on the 7th day. The calculation formula is as follows:

[0097] Germination potential (%) = number of seeds germinated in 3 days / total number of experimental seeds × 100

[0098] Germination rate (%) = number of seeds germinated in 7 days / total number of experimental seeds × 100

[0099]

[0100] 5.2. Experiment on cucumber seeds at seedling stage with different coating treatments

[0101] 48 to 72 hours after the seeds begin to germinate, they are transplanted into plug trays and placed in a constant temperature incubator at 25℃±10℃. The cucumber growth indicators are measured every 7 days, and the chlorophyll content of the cucumber is measured after 35 days.

[0102] Cucumber growth index determination:

[0103] In the seedling experiment, cucumber growth indicators, including plant height, stem diameter and leaf area, were measured every 7 days. Plant height was measured with a ruler, stem diameter was measured with a vernier caliper, and leaf area was measured by leaf length and leaf width. Leaf area was calculated using the formula: leaf area = leaf length × leaf width × leaf area index. After 35 days, the chlorophyll content of cucumber was measured using a chlorophyll meter.

[0104] 6. Test results

[0105] 6.1. Effects of sodium hypochlorite seed coating on cucumber seed germination and seedling growth

[0106] 6.1.1. Effects of Sodium Hypochlorite Seed Coating on Cucumber Seed Germination

[0107] Table 1 Effect of sodium hypochlorite seed coating on cucumber seed germination

[0108] Group No. Processing Germination potential / % Germination rate / % Germination Index GI 1 0.2％ 46.00％ 90.00％ 36.94 2 0.3％ 61.29％ 91.67％ 38.05 3 0.4％ 64.58％ 92.00％ 41.56 4 0.5％ 69.39％ 93.88％ 49.51 5 0.6％ 55.10％ 79.59％ 37.67 CK none 33.33％ 83.33％ 26.75

[0109] As shown in Table 1, from the perspective of germination potential, the germination potential of cucumber seeds treated with sodium hypochlorite in the concentration range of 0.2%-0.6% is greater than that of the blank treatment group. As the sodium hypochlorite content increases, the germination potential of cucumber seeds generally increases first and then decreases. Among them, the germination potential of cucumber seeds treated with 0.6% sodium hypochlorite concentration is lower, which is inconsistent with the previous upward trend. Under the treatment of 0.5% sodium hypochlorite concentration, the germination potential of cucumber seeds is the highest, reaching 69.39%.

[0110] From the perspective of germination rate, the germination rates of 0.2%, 0.3%, 0.4% and 0.5% sodium hypochlorite treatments were higher than those of the blank control group, while the germination rate of 0.6% sodium hypochlorite treatment was different from that of the blank control group. The highest germination rate was 0.5% concentration treatment, reaching 93.88%.

[0111] From the perspective of germination index, the germination index of seeds treated with different concentrations of sodium hypochlorite was higher than that of the blank control group. The treatment with the highest germination index was 0.5% sodium hypochlorite concentration, with a germination index of 49.51. Overall, 0.5% is a more suitable concentration for the treatment of cucumber seeds with sodium hypochlorite seed coating.

[0112] 6.1.2. Effect of Sodium Hypochlorite Seed Coating on Cucumber Seedling Height

[0113] Depend on Figure 1 It can be seen that, taking April 13 as an example, when the sodium hypochlorite concentration was in the range of 0.4%-0.6%, the cucumber plant height was higher than that of the blank treatment group. In general, when the sodium hypochlorite concentration was in the range of 0.2%-0.5%, the plant height increased with the increase of sodium hypochlorite concentration, but it decreased at 0.3% sodium hypochlorite concentration. When the sodium hypochlorite concentration was 0.5%-0.6%, the plant height began to decrease with the increase of sodium hypochlorite concentration. When the sodium hypochlorite concentration was 0.5%, the plant height reached the maximum, and the average plant height of cucumbers treated with 0.5% sodium hypochlorite on April 13 reached 9.10cm. When the sodium hypochlorite concentration was 0.3%, the plant height was the minimum, and the average plant height of cucumbers treated with 0.3% sodium hypochlorite on April 13 reached 6.8cm.

[0114] 6.1.3. Effect of Sodium Hypochlorite Seed Coating on Stem Diameter of Cucumber Seedlings

[0115] Depend on Figure 2 It can be seen that there is no significant regularity in the effect of sodium hypochlorite concentration on the stem thickness of cucumber seedlings, and the effect is not obvious.

[0116] 6.1.4. Effect of Sodium Hypochlorite Seed Coating on Leaf Area of ​​Cucumber Seedlings

[0117] Table 2 Effect of sodium hypochlorite seed coating on leaf area of ​​cucumber seedlings (cm 2 )

[0118]

[0119] As shown in Table 2, the leaf area of ​​cucumbers treated with 0.2%-0.6% sodium hypochlorite is higher than that of the blank control group, indicating that sodium hypochlorite can promote the growth of cucumber leaf organs to a certain extent. Taking April 13 as an example, when the sodium hypochlorite concentration was within the range of 0.2%-0.6%, the cucumber leaf area also increased with the increase of sodium hypochlorite concentration. When the sodium hypochlorite concentration was 0.6%, the leaf area was the largest, which was 28.19 cm 2 , so the optimum concentration is 0.6%.

[0120] 6.1.5. Effect of Sodium Hypochlorite Seed Coating on Chlorophyll Content in Cucumber Seedlings

[0121] Depend on Figure 3It can be seen that the chlorophyll content of cucumbers treated with 0.2%-0.6% sodium hypochlorite concentration is higher than that of the blank control group, so sodium hypochlorite can effectively increase the chlorophyll content of cucumbers. Among them, the chlorophyll content in the 0.2%-0.5% sodium hypochlorite concentration treatment range increases with the increase of sodium hypochlorite concentration, reaching the highest at a concentration of 0.5%, with a chlorophyll content of 32.8SPAD. The chlorophyll content in the 0.5%-0.6% sodium hypochlorite concentration treatment range decreases with the increase of sodium hypochlorite concentration, so the optimal concentration is 0.5%.

[0122] 6.2. Effects of chlormequat seed coating on cucumber seed germination and seedling growth

[0123] 6.2.1. Effects of chlormequat seed coating on cucumber seed germination

[0124] Table 3 Effect of chlormequat seed coating agent on cucumber seed germination

[0125] Group No. Processing Germination potential / % Germination rate / % Germination Index GI 1 0.004％ 50.00％ 90.00％ 31.64 2 0.006％ 61.29％ 96.77％ 36.05 3 0.008％ 66.67％ 83.33％ 30.40 4 0.010％ 43.33％ 83.33％ 25.87 5 0.012％ 93.33％ 100.00％ 44.37 6 CK 33.33％ 83.33％ 26.75

[0126] As shown in Table 3, from the perspective of germination potential, the germination potential of cucumber seeds treated with 0.04%-0.012% chlormequat is greater than that of the blank treatment group. As the chlormequat content increases, the germination potential of cucumber seeds increases as a whole. Among them, the germination potential of cucumber seeds treated with 0.010% chlormequat concentration is lower, which is inconsistent with the trend. Under the treatment of 0.012% chlormequat concentration, the germination potential of cucumber seeds is the highest, reaching 93.33%, which is the optimal concentration.

[0127] From the germination rate, the germination rate of 0.004%, 0.006%, 0.012% chlormequat treatment was higher than that of the blank control group, while the germination rate of 0.008%, 0.012% chlormequat treatment was the same as that of the blank control group. The highest germination rate was 0.012% concentration treatment, which reached 100%.

[0128] From the germination index, except for the seeds treated with 0.010% chlormequat, the germination index of other seeds was higher than that of the blank control group. The treatment with the highest germination index was also 0.012% chlormequat, with a germination index of 44.37, so 0.012% was the optimal concentration.

[0129] 6.2.2. Effect of chlormequat seed coating on the height of cucumber seedlings

[0130] Depend on Figure 4It can be seen that within the concentration range of 0.004%-0.012% of chlormequat, the height of cucumber plants was lower than that of the blank control group, indicating that chlormequat can effectively reduce the height of cucumber seedlings and alleviate the phenomenon of cucumber leggy growth. Within the concentration range of 0.04%-0.012%, as the concentration of chlormequat increases, the height of cucumber seedlings decreases. Taking April 13 as an example, when the concentration of chlormequat is 0.012%, the height of cucumber plants is the lowest, which is 7.23cm.

[0131] 6.2.3. Effect of chlormequat seed coating on stem diameter of cucumber seedlings

[0132] Depend on Figure 5 It can be seen that in the early growth period of cucumber seeds (i.e. the first 7-day period after germination), chlormequat has a certain degree of inhibitory effect on the stem thickness of cucumbers. The stem thickness of cucumbers treated with chlormequat concentrations ranging from 0.004% to 0.012% is lower than that of the blank control group, and with the increase of chlormequat concentration, the stem thickness of cucumber seeds shows a downward trend. However, as the cucumbers grow further, high concentrations of chlormequat promote the thickening of the stems. From the second 7-day period to the fifth 7-day period, with the increase of chlormequat, the stem thickness of cucumbers shows an upward trend. Taking April 13 as an example, the stem thickness of cucumbers treated with chlormequat concentrations ranging from 0.006% to 0.012% is lower than that of the blank control group, and with the increase of chlormequat concentration, the stem thickness shows an upward trend.

[0133] 6.2.4. Effect of chlormequat seed coating on leaf area of ​​cucumber seedlings

[0134] Table 4 Effect of chlormequat seed coating on leaf area of ​​cucumber seedlings (cm 2 )

[0135]

[0136] As shown in Table 4, in the early and middle stages of cucumber growth (week 1 to week 3), chlormequat has a certain inhibitory effect on the leaf area of ​​cucumber. During this period, the leaf area of ​​cucumbers treated with chlormequat concentrations in the range of 0.004%-0.012% was lower than that of the blank control group. However, in the late stage of cucumber growth (week 4 to week 5), the leaf area of ​​cucumbers treated with chlormequat concentrations in the range of 0.006%-0.010% was higher than that of the blank control group; the leaf area of ​​cucumbers treated with chlormequat concentrations in the range of 0.004%-0.010% showed an upward trend with the increase of chlormequat concentration, and the leaf area reached the maximum at a concentration of 0.010%, which was 24.75 cm 2 The leaf area of ​​cucumbers treated with chlormequat concentrations ranging from 0.010% to 0.012% decreased with the increase of chlormequat concentration. In terms of leaf area, the optimum concentration of chlormequat is 0.010%.

[0137] 6.2.5. Effects of chlormequat seed coating on chlorophyll content in cucumber seedlings

[0138] Depend on Figure 6 It can be seen that when the concentration of chlormequat is in the range of 0.004%-0.012%, the chlorophyll content of cucumber is higher than that of the blank control group, and as the concentration of chlormequat increases, the chlorophyll concentration also shows an upward trend. Therefore, it can be seen that chlormequat can effectively increase the chlorophyll content of cucumber.

[0139] 6.3. Effects of boric acid seed coating on cucumber seed germination and seedling growth

[0140] 6.3.1. Effects of boric acid seed coating on cucumber seed germination

[0141] Table 5 Effect of boric acid seed coating on cucumber seed germination

[0142] Group No. Processing Germination potential / % Germination rate / % Germination Index GI 1 0.006％ 98.33％ 100.00％ 48.08 2 0.012％ 93.33％ 95.00％ 48.15 3 0.018％ 98.33％ 96.67％ 49.24 4 0.024％ 96.67％ 98.33％ 48.05 5 0.030％ 95.00％ 96.67％ 47.85 6 CK 81.67％ 86.67％ 31.28

[0143] As shown in Table 5, boric acid seed coating has a significant promoting effect on seed germination potential. The germination potential of boric acid in the range of 0.006%-0.030% is higher than that of the blank treatment group, and the germination potential is the highest at 98.33% when the boric acid concentration is 0.006% and 0.018%.

[0144] In terms of germination rate, the germination rate of the boric acid treatment group within the boric acid concentration range of 0.006%-0.030% is higher than that of the blank treatment group, so boric acid can effectively improve the germination rate of cucumbers. Among them, the germination rate is the highest in the 0.006% boric acid treatment group, which is as high as 100%. In addition, except for the 0.006% boric acid treatment group, the germination rate of cucumbers treated in the range of 0.012%-0.024% boric acid increases with the increase of boric acid concentration, among which the germination rate of cucumbers treated with 0.024% boric acid is the highest, reaching 98.33%; the germination rate of cucumbers treated in the range of 0.024%-0.030% boric acid decreases with the increase of boric acid concentration.

[0145] In terms of germination index, the germination index of the boric acid concentration range of 0.006%-0.030% was higher than that of the blank treatment group, so boric acid can effectively improve the germination ability of cucumbers. For cucumbers treated with boric acid concentrations of 0.006%-0.018%, the germination index showed an upward trend as the boric acid concentration increased, among which the germination index of cucumbers treated with 0.018% boric acid was the largest, reaching 49.24; for cucumbers treated with boric acid concentrations of 0.018%-0.030%, the germination index showed a downward trend as the boric acid concentration increased. Therefore, in terms of germination index, 0.018% boric acid concentration treatment was the most suitable.

[0146] 6.3.2. Effect of boric acid seed coating on cucumber seedling height

[0147] Depend on Figure 7It can be seen that in the early growth period (week 1 and week 2), boric acid can effectively increase plant height. In the range of 0.006%-0.018% boric acid concentration, as the boric acid concentration increases, the cucumber plant height also shows an upward trend. In the range of 0.018%-0.030% boric acid concentration, as the boric acid concentration increases, the cucumber plant height shows a downward trend. Among them, in the first cycle, the cucumber plant height in the range of 0.006%-0.024% boric acid concentration is higher than that of the blank control group, and the cucumber treated with 0.030% boric acid concentration is lower than that of the control group; in the second cycle, the cucumber plant height in the range of 0.012%-0.030% boric acid concentration is higher than that of the blank control group, and the cucumber plant height treated with 0.006% boric acid concentration is lower than that of the control group.

[0148] In the middle and late stages of growth, the effect of boric acid on plant height gradually decreased. Taking April 13 as an example, within the range of 0.006%-0.018% boric acid concentration, as the boric acid concentration increased, the height of cucumber plants also showed an upward trend; at boric acid concentrations of 0.018% and 0.024%, the height of cucumber plants remained the same; within the range of 0.024%-0.030% boric acid concentration, as the boric acid concentration increased, the height of cucumber plants showed a downward trend. Among them, the height of cucumber plants in the range of 0.012%-0.024% boric acid concentration was higher than that of the blank control group, and the height of cucumbers treated with 0.006% and 0.030% boric acid concentrations was lower than that of the control group.

[0149] 6.3.3. Effect of boric acid seed coating on stem diameter of cucumber seedlings

[0150] Depend on Figure 8 It can be seen that boric acid treatment has no significant promoting effect on cucumber stem thickness, and there is no obvious pattern in the effects of each treatment on stem thickness. Taking April 6 as an example, the cucumber stem thickness within the boric acid concentration range of 0.006%-0.030% is smaller than that of the blank control group, and the cucumber stem thickness value is the largest when the boric acid seed coating concentration is 0.018%.

[0151] 6.3.4. Effect of boric acid seed coating on leaf area of ​​cucumber seedlings

[0152] Table 6 Effect of boric acid seed coating on leaf area of ​​cucumber seedlings (cm 2 )

[0153]

[0154] As shown in Table 6, boric acid has different effects on cucumber leaf area in the early and middle growth stages (week 1 to week 3). During this period, the cucumber leaf area treated with boric acid concentrations in the range of 0.006%-0.030% had different changes compared with the blank control group.

[0155] In the late growth period of cucumber (week 4 to week 5), the leaf area of ​​cucumbers treated with boric acid concentrations ranging from 0.006% to 0.018% was higher than that of the blank control group. The leaf area of ​​cucumbers treated with boric acid concentrations ranging from 0.006% to 0.018% showed an increasing trend with the increase of boric acid concentration, and reached the maximum leaf area of ​​29.42 cm at a concentration of 0.018%. 2 ; The leaf area of ​​cucumbers treated with boric acid concentrations in the range of 0.018%-0.030% decreased with the increase of boric acid concentration. In terms of leaf area, the optimum concentration of boric acid is 0.018%.

[0156] 6.3.5. Effect of boric acid seed coating on chlorophyll content in cucumber seedlings

[0157] Depend on Fig. 9 It can be seen that the chlorophyll content of cucumbers in the range of 0.006%-0.030% boric acid concentration is higher than that of the blank control group, so boric acid has a promoting effect on chlorophyll content. In the range of 0.006%-0.012% boric acid concentration, as the boric acid concentration increases, the chlorophyll content of cucumbers also shows an upward trend; in the range of 0.012%-0.030% boric acid concentration, as the boric acid concentration increases, the chlorophyll content of cucumbers shows a downward trend. Among them, when the boric acid concentration is 0.012%, the chlorophyll content of cucumbers is the highest, which is 34.90SPAD.

[0158] 6.4. Effects of compound seed coating agents on cucumber seed germination and seedling growth

[0159] 6.4.1. Effects of compound seed coating agents on cucumber seed germination

[0160] Table 7 Effect of compound seed coating agent on cucumber seed germination

[0161]

[0162]

[0163] It can be seen from Table 7 that the compound seed coating agent has a promoting effect on the germination rate, germination potential and germination index of cucumber seeds.

[0164] From the perspective of germination potential, except for the four treatment groups No. 5, No. 6, No. 10, No. 12, and No. 14, which were significantly lower than the blank control group, the germination potential of the other treatment groups was significantly higher than the blank control group. Among them, the treatment groups with the best germination potential of cucumber were No. 13, No. 22, and No. 26, which were 36%-37.5% higher than the blank control group, and there was no significant difference among the three treatments.

[0165] From the germination rate point of view, treatment groups No. 1, 2, 4, 9, 11, 13, 19, 20, 25, 26 and 27 were significantly higher than the blank control group, 3%-11% higher than the blank control group; treatment groups No. 7, 12, 15, 16, 17, 21 and 22 had no significant difference compared with the blank control group; treatment groups No. 3, 5, 6, 8, 10, 14, 18, 23 and 24 were significantly lower than the blank control group.

[0166] From the germination index point of view, the germination index of all treatment groups was significantly greater than that of the blank treatment group, and the germination index of treatment group 2 was the highest, which was 69.87, significantly higher than that of other treatment groups.

[0167] Combining the three indexes, it can be seen that treatment groups 1, 4, 9, 13, 19 and 26 performed best in cucumber germination.

[0168] 6.4.2. Effects of compound seed coating agents on the height of cucumber seedlings

[0169] Table 8 Effect of compound seed coating agent on height of cucumber seedlings (cm)

[0170]

[0171]

[0172] As shown in Table 8, the effect of the composite seed coating agent on cucumber plant height was mainly concentrated in the early and middle stages (week 1, week 2, and week 3). On March 16, the plant heights of treatments No. 1, 2, 8, 9, 11, 15, 16, 17, 19, and 26 were significantly lower than those of the blank control group, 0.3-1.2 cm lower than the blank treatment group, and the plant heights of treatments No. 17 and 19 were the lowest, and there was no significant difference among the treatments; on March 23, the plant heights of treatments No. 1, 2, 6, 7, 8, 9, 11, 15, 16, 18, 19, 21, 26, and 27 were significantly lower than those of the blank control group, 0.4-1.8 cm lower than the blank treatment group, and the plant height of treatment No. 19 was the lowest; on March 30, the plant heights of treatments No. 1, 2, 6, 7, 8, 9, 11, 15, 16, 18, 19, 21, 26, and 27 were significantly lower than those of the blank control group, 0.4-1.8 cm lower than the blank treatment group, and the plant height of treatment No. 19 was the lowest; The plant heights of treatments No. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 18, 19, 20, 21, 26 and 27 were significantly lower than those of the blank control group, 0.8-3.1 cm lower than the blank treatment group, and the plant height of treatment No. 20 was the lowest, which was 4.38 cm; on April 6, except for treatment groups No. 1, 2, 3, 4 and 25, the plant heights of all other treatment groups were not significantly lower than those of the blank control group, which were 1.1-1.7 cm lower than the blank control group, and the plant height of treatment group No. 3 was the lowest, which was 5.95 cm; on April 13, the plant heights of treatments No. 1, 2 and 27 were significantly lower than those of the blank control group, 0.8-1.8 cm lower than the blank treatment group.

[0173] In summary, it can be clearly seen that the effect of the composite seed coating agent on the height of cucumber plants is mainly concentrated in the early and middle stages (week 1, week 2, and week 3). In the first three cycles, in the first cycle, 10 treatment groups had significantly lower plant heights than the blank control group, in the second cycle, 14 treatment groups had significantly lower plant heights than the blank control group, and in the third cycle, 21 treatment groups had significantly lower plant heights than the blank control group. In the last two cycles, only 5 and 3 treatment groups had significantly lower plant heights than the blank control group, and the number of treatment groups with plant heights lower than the blank control group decreased significantly, indicating that the effect of dwarfing on plant height was weakened. Among them, the treatment groups that significantly inhibited the phenomenon of tall seedlings in the early growth stage were treatment groups 1, 2, 6, 7, 8, 9, 11, 15, 16, 18, 19, 21, 26, and 27, which performed well in inhibiting leggy growth.

[0174] 6.4.3. Effect of compound seed coating agent on stem diameter of cucumber seedlings

[0175] The compound seed coating agent had no significant effect on the stem thickness of cucumber seedlings.

[0176] 6.4.4. Effect of compound seed coating agent on leaf area of ​​cucumber seedlings

[0177] Table 9 Effect of compound seed coating agent on leaf area of ​​cucumber seedlings (cm 2 )

[0178]

[0179]

[0180] As shown in Table 9, the compound seed coating agent has an inhibitory effect on the leaf area of ​​cucumber in the early and middle growth stages, and begins to promote the growth of the leaf area of ​​cucumber in the late growth stages. In the first cycle, only the leaf areas of cucumbers in treatments No. 16 and No. 18 were significantly higher than those in the blank control group; in the second cycle, only the leaf areas of cucumbers in treatments No. 21 and No. 27 were significantly higher than those in the blank control group; in the third cycle, only the leaf areas of cucumbers in treatments No. 15 and No. 18 were significantly higher than those in the blank control group. This shows that the leaf areas of cucumbers treated with most mixed seed coating agents were inhibited, with no significant difference from the blank control group or even significantly lower than those in the blank control group. In the fourth week, treatments No. 2, No. 3, No. 4, No. 6, No. 7, No. 8, No. 12, No. 15, No. 16, No. 17, No. 18, No. 20, No. 22, No. 23, No. 24, No. 25, and No. 27 were significantly higher than the blank control group, 3-15.6 cm higher than the blank control group. 2 , among which the treatment group No. 15 had the highest value of 29.53cm 2 At week 5, the treatment groups No. 2, No. 3, No. 6, No. 8, No. 12, No. 15, No. 16, No. 17, No. 18, No. 19, No. 20, No. 21, No. 22, No. 23, No. 24, No. 25, No. 26, and No. 27 were significantly higher than the blank control group, 3-13.6 cm higher than the blank control group 2 Among them, the leaf areas of treatment groups No. 15, No. 18, No. 25, and No. 26 were the largest, and there was no significant difference among the treatments.

[0181] In summary, the treatment groups that promoted significant growth of cucumber leaf area in the later growth period were No. 2, No. 3, No. 6, No. 8, No. 12, No. 15, No. 16, No. 17, No. 18, No. 20, No. 22, No. 23, No. 24, No. 25, No. 26, and No. 27, and among them, the leaf area growth of treatment groups No. 15, No. 18, No. 25, and No. 26 was the largest.

[0182] 6.4.5. Effect of compound seed coating agent on chlorophyll content of cucumber seedlings

[0183] Table 10 Effect of compound seed coating agent on chlorophyll content of cucumber seedlings

[0184]

[0185]

[0186] As shown in Table 10, the chlorophyll content of all treatment groups was higher than that of the blank control group, so the compound seed coating agent promoted the chlorophyll content of cucumber. Among them, the chlorophyll content of cucumbers in treatments No. 1, No. 4, No. 5, No. 7, No. 9, No. 13, No. 14, No. 15, No. 16, No. 18, No. 19, No. 21, No. 23, No. 26, and No. 27 was significantly higher than that in the blank control group, 3.4-14.1 higher than that in the blank control group. Among them, the chlorophyll content of treatments No. 14, No. 21, and No. 23 was the most significant, and there was no significant difference between the treatments, which was 11.3-14.1 higher than that in the blank control group.

[0187] In summary, No. 9 (0.018% H3BO3 + 0.3% NaClO + 0.010% C5H 13 Cl2N), No. 26 (0.012%H3BO3+0.5%NaClO+0.010%C5H 13 Cl2N), No. 27 (0.018%H3BO3+0.5%NaClO+0.010%C5H 13 The Cl2N) treatment group performed significantly better than the blank control group in the four indicators of germination, plant height, leaf area and chlorophyll content. Therefore, the three compound seed dressing ratios of No. 9, No. 26 and No. 27 were the most suitable ratios.

[0188] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A cucumber seed coating agent, characterized in that: By mass concentration, it includes active ingredients: sodium hypochlorite 0.3%-0.5%, chlormequat chloride 0.006%-0.01%, boric acid 0.012%-0.018% and inactive ingredients.

2. The cucumber seed coating agent according to claim 1, characterized in that In terms of mass concentration, the active ingredients include 0.3% sodium hypochlorite, 0.01% chlormequat chloride and 0.018% boric acid; or 0.5% sodium hypochlorite, 0.01% chlormequat chloride and 0.012% boric acid; or one of 0.5% sodium hypochlorite, 0.01% chlormequat chloride and 0.018% boric acid.

3. The cucumber seed coating agent according to claim 1, characterized in that The inactive ingredients include dispersants, antifreeze agents, anticaking agents, film formers, preservatives, warning colors, fillers and water.

4. The cucumber seed coating agent according to claim 3, characterized in that Among the inactive ingredients, the mass ratio of dispersant, antifreeze agent, anticaking agent, film former, preservative, warning color and filler is 40-60:0.8-1.2:0.8-1.2:40-60:1.6-2.4:80-120:8-12.

5. The cucumber seed coating agent according to claim 3, characterized in that The dispersant is selected from one or more of sodium lignin sulfonate, pull open powder BX, and sodium N-methyl-fatty acyl-taurate; The antifreeze agent is selected from one or more of ethylene glycol, propylene glycol and glycerol; The anticaking agent is selected from one or more of silicon dioxide, talc, and magnesium stearate; The film-forming agent is selected from one or more of polyvinyl alcohol, sodium carboxymethyl cellulose, and sodium polyacrylate; The preservative is selected from one or more of sodium benzoate, potassium sorbate, and sodium dehydroacetate; The warning color is selected from one or more of acid scarlet, amaranth, and carmine; The filler is selected from one or more of light calcium carbonate, white carbon black and kaolin.

6. The method for preparing the cucumber seed coating agent according to any one of claims 1 to 5, characterized in that: The following steps are involved: Weigh the dispersant, film-forming agent and preservative in proportion, add water and stir evenly; Weigh the warning color and dissolve it in water; Weighing an anticaking agent, an antifreeze agent and a filler, together with the warning color solution of step (2), add them to the mixed solution of step (1), and stir evenly to obtain an inactive ingredient slurry; According to the concentration, active ingredients sodium hypochlorite, chlormequat chloride and boric acid are added to the slurry of the inactive ingredients to obtain the cucumber seed coating agent.

7. Use of the cucumber seed coating agent according to any one of claims 1 to 5 for treating cucumber seeds.

8. The use of the cucumber seed coating agent according to claim 7 for cucumber seed treatment, characterized in that: The cucumber seed coating agent is mixed with cucumber seeds at a mass ratio of 1:30-50, and the seeds are soaked and coated.

9. The use of the cucumber seed coating agent according to claim 7 for cucumber seed treatment, characterized in that: The method also includes taking out the seeds and drying them after soaking and coating the seeds to obtain coated cucumber seeds.

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