A kind of pyruvate assay kit and its preparation method and application

Abstract

The present invention provides a pyruvic acid determination reagent box. The reagent box comprises a reagent R1 and a reagent R2. The reagent R1 comprises the following ingredients: reduced coenzyme I(NADH), a trihydroxymethyl aminomethane (Tris) buffer solution, a surface active agent, EDTA-Na2 and a preservative. The reagent R2 comprises the following ingredients: the trihydroxymethyl aminomethane (Tris) buffer solution, lactate dehydrogenase (LDH), the surface active agent, a stabilizer and the preservative. The present also provides a preparation method of the reagent box and applications. The reagent box is a high-stability, high-accuracy and good-repeatability liquid reagent box.

Description

technical field [0001] The present invention relates to the technical field of biochemical reagent assay, in particular to a pyruvate assay kit, and also relates to a preparation method and application of the pyruvate assay kit. Background technique [0002] Pyruvic acid (Pyruvic acid), also known as 2-oxopropionic acid, is one of the intermediate products involved in the basic metabolism of the whole organism. It can realize the transition between sugar, fat and tricarboxylic acid cycle in the body through acetyl-CoA and tricarboxylic acid cycle. The transformation of nutrients plays an important pivotal role in the connection of the three major nutrients. Under normal circumstances, the human body can be oxidized into CO2 and water through the tricarboxylic acid cycle, so that the ratio of lactic acid / pyruvate in the blood can be maintained at about 9. However, when the body is in a state of hypoxia, pyruvate is reduced to lactic acid, and the ratio increases, and the mor...

AI Technical Summary

Problems solved by technology

The dinitrophenylhydrazine colorimetric method is based on the reaction of pyruvic acid and 2,4-dinitrophenylhydrazine to generate colored phenylhydrazone, but this method is interfered by other α-keto acids, has poor specificity, and is cumbersome to operate

Enzyme-linked immunoassay has high sensitivity and specificity, but there are many interference factors, poor repeatability, and high price; fluorescence analysis method has high accuracy and sensitivity, but it is inconvenient to store reagents, and it is manual operation, which takes a long time. The price is high; the lactate dehydrogenase method has the advantages of high specificity, simple and fast operation, accuracy and safety, automatic analysis, and low cost. However, the existing Chinese-made kits have disadvantages such as poor stability and repeatability. The present invention improves the shortcomings of the existing pyruvate kits to meet the requirements of clinical testing and chemical analysis

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