Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A novel assay kit for serum alpha-fetoprotein heterogeneity 3 and its preparation method and application

A technology of alpha-fetoprotein and heterogenous body, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of high price, expensive equipment and inconvenient storage of reagents, etc.

Active Publication Date: 2021-04-20
中拓生物有限公司 +2
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current method for detecting alpha-fetoprotein isoform 3 is mainly double-antibody sandwich immunochemiluminescence assay (ILMA), which has high accuracy and sensitivity, but the equipment is expensive, the reagents are inconvenient to store, and the price is high
Latex immunoturbidimetric method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. However, there is no AFP-L3 biochemical kit in China, only AFP kit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A novel assay kit for serum alpha-fetoprotein heterogeneity 3 and its preparation method and application
  • A novel assay kit for serum alpha-fetoprotein heterogeneity 3 and its preparation method and application
  • A novel assay kit for serum alpha-fetoprotein heterogeneity 3 and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A conventional assay kit for alpha-fetoprotein heterosome 3, which includes reagent R1 and reagent R2.

[0052] Reagent R1 contains the following components:

[0053] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0054] NaCl 9g / L;

[0055] Bovine serum albumin (BSA) 10g / L;

[0056] Polyethylene glycol 6000 8g / L;

[0057] Trehalose 8g / L;

[0058] GLAURIN 1%;

[0059] proclin300 1 ml / L.

[0060] Reagent R1 has a pH value of 7.0.

[0061] Reagent R2 contains the following ingredients:

[0062] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0063] Goat anti-human AFP-L3 antibody coated latex particles 15mg / L;

[0064] Goat anti-human AFP-L3 antibody coated magnetic nanoparticles 15mg / L;

[0065] Bovine serum albumin (BSA) 5g / L;

[0066] Polyethylene glycol 6000 5g / L;

[0067] Trehalose 5g / L;

[0068] GLAURIN 1%;

[0069] proclin300 1ml / L.

[0070] Reagent R2 has a pH of 6.8.

[0071] Wherein said percentag...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The present invention provides a kit for measuring alpha-fetoprotein heterosome 3 (AFP‑L3), which contains reagent R1 and reagent R2; reagent R1 contains the following components: piperazine‑1,4‑diethanesulfonic acid (PIPES ) buffer, NaCl, surfactant, suspending agent, preservative. Reagent R2: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, goat anti-human AFP-L3 antibody coated latex particles, goat anti-human AFP-L3 antibody coated magnetic nanoparticles, suspending agent, stabilizer Agents, preservatives. The invention also provides the preparation method and application of the kit. The kit can effectively replace the kit of a chemiluminescence immunoassay analyzer, and is a novel liquid kit with strong stability, high sensitivity, good repeatability and low cost. It is convenient for the development and application of the project in clinic.

Description

technical field [0001] The present invention relates to the technical field of biochemical reagent determination, in particular to a kit for measuring alpha-fetoprotein heterogeneity 3, and also relates to a preparation method and application of the kit for determination of alpha-fetoprotein heterogeneity 3. Background technique [0002] Primary hepatocellular carcinoma (HCC) is one of the common malignant tumors in my country, with a high mortality rate, ranking third in the death order of malignant tumors after gastric cancer and esophageal cancer. my country is a big country with hepatitis B, and there are about 120 million cases of hepatitis B virus carriers. Every year, 130,000 patients die of liver cancer, accounting for 45% of the world's liver cancer deaths. Like other tumor screenings, the prevention and treatment of liver cancer mainly lies in the "three early", that is, early detection, early diagnosis and early treatment. Early liver cancer, if treated scientifi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/543G01N33/531
CPCG01N33/531G01N33/54326G01N33/54346G01N33/57438G01N33/57476
Inventor 刘安娜张刘云
Owner 中拓生物有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com